Anti-IGFBP3 Antibody Picoband™

Product Name

Anti-IGFBP3 Antibody Picoband™

View all IGFBP-3 Antibodies

SKU/Catalog Number

A00435-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IGFBP3 Antibody Picoband™ catalog # A00435-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IGFBP3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00435-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IGFBP3 recombinant protein (Position: A29-K267).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00435-2 is reactive to IGFBP3 in Human

Applications

A00435-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

31.674kDa

Background of IGFBP-3

IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, human
ELISA (Cap), 1-5μg/ml, human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human IGFBP3 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # A00435-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 37KD. The expected band size for IGFBP3 is at 29KD.

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Figure 2. Sandwich ELISA - Recombinant human IGFBP3 protein standard curve.
Use in combination with reagents from Human IGFBP3 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0386).

Gene/Protein Information For IGFBP3 (Source: Uniprot.org, NCBI)

Alternative Names

acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3 IGFBP3 BP-53, IBP3 insulin like growth factor binding protein 3 insulin-like growth factor-binding protein 3|IBP-3|IGF-binding protein 3|IGFBP-3|acid stable subunit of the 140 K IGF complex|binding protein 29|binding protein 53|growth hormone-dependent binding protein

For more info on IGFBP3, check out the IGFBP3 Infographic

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In this infographic, you will see the following information for IGFBP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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