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Product Info Summary
| SKU: | A30651 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RPC1 POLR3A Antibody
SKU/Catalog Number
A30651
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RPC1 POLR3A Antibody (Catalog# A30651). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPC1 POLR3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30651)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPC1. Immunogen sequence location: 31-80
Reactive Species
A30651 is reactive to POLR3A in Human, Monkey, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
155.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30651 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using POLR3A Polyclonal Antibody.
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Western blot analysis of the lysates from 293 cells using RPC1 antibody.
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Western blot analysis of lysates from COS and HUVEC cells, using RPC1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-RPC1 POLR3A Antibody (A30651)
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1 Customer Q&As for Anti-RPC1 POLR3A Antibody
Question
We are currently using anti-RPC1 antibody A30651 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The anti-RPC1 antibody (A30651) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-07
