Anti-mTOR/Tor Rabbit Monoclonal Antibody

Boster Bio Anti-mTOR/Tor Rabbit Monoclonal Antibody catalog # M00003. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: M00003
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-mTOR/Tor Rabbit Monoclonal Antibody

See all TOR/mTOR products

SKU/Catalog Number

M00003

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-mTOR/Tor Rabbit Monoclonal Antibody catalog # M00003. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-mTOR/Tor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00003)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CBD-13

Immunogen

A synthesized peptide derived from human mTOR

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00003 is reactive to MTOR in Human, Mouse, Rat

Applications

M00003 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:30
Flow Cytometry 1:20

Validation Images & Assay Conditions

Gene/Protein Information For MTOR (Source: Uniprot.Org, NCBI)

Uniprot ID

P42345

Gene ID

2475

Gene Name

MTOR

Full Name

Serine/threonine-protein kinase mTOR

Weight

288.892kDa

Superfamily

PI3/PI4-kinase family

Alternative Names

EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/thr MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MTOR, check out the MTOR Infographic

MTOR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MTOR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00003 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Nie L,Liu M,Chen J,Wu Q,Li Y,Yi J,Zheng X,Zhang J,Chu C,Yang J.Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.Mol Med Rep.2021 Apr;23(4):299.doi:10.3892/mmr.2021.11938.Epub 2021 Mar 2.PMID:336498
Species: Rat
M00003 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:1:500

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
M00003 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

Correlation between autophagy of osteoblasts and oxidative stress of osteoporosis rats

Rapamycin provides anti%u2011epileptogenic effect in a rat model of post%u2011traumatic epilepsy via deactivation of mTOR signaling pathway

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17 Customer Q&As for Anti-mTOR/Tor Rabbit Monoclonal Antibody

Question

Would anti-mTOR/Tor Rabbit Monoclonal antibody M00003 work on pig IHC with testis?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

Our lab technicians have not tested anti-mTOR/Tor Rabbit Monoclonal antibody M00003 on pig. You can run a BLAST between pig and the immunogen sequence of anti-mTOR/Tor Rabbit Monoclonal antibody M00003 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig testis in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-27

Question

I see that the anti-mTOR/Tor Rabbit Monoclonal antibody M00003 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-22

Question

Is a blocking peptide available for product anti-mTOR/Tor Rabbit Monoclonal antibody (M00003)?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

We do provide the blocking peptide for product anti-mTOR/Tor Rabbit Monoclonal antibody (M00003). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-23

Question

Do you have a BSA free version of anti-mTOR/Tor Rabbit Monoclonal antibody M00003 available?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-mTOR/Tor Rabbit Monoclonal antibody M00003 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-13

Question

I was wanting to use your anti-mTOR/Tor Rabbit Monoclonal antibody for WB for mouse cerebellum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cerebellum identification?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

It shows on the product datasheet, M00003 anti-mTOR/Tor Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cerebellum in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-25

Question

We have seen staining in rat cervix carcinoma. What should we do? Is anti-mTOR/Tor Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

From what I have seen in literature cervix carcinoma does express MTOR. From what I have seen in Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-09-16

Question

Would anti-mTOR/Tor Rabbit Monoclonal antibody M00003 work for WB with cerebellum?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

According to the expression profile of cerebellum, MTOR is highly expressed in cerebellum. So, it is likely that anti-mTOR/Tor Rabbit Monoclonal antibody M00003 will work for WB with cerebellum.

Boster Scientific Support

Answered: 2019-07-26

Question

you antibody using your anti-mTOR/Tor Rabbit Monoclonal antibody for pip3 activates akt signaling studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

We appreciate your inquiry. This M00003 anti-mTOR/Tor Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-18

Question

Does M00003 anti-mTOR/Tor Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

As indicated on the product datasheet, M00003 anti-mTOR/Tor Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-11

Question

Our team were satisfied with the WB result of your anti-mTOR/Tor Rabbit Monoclonal antibody. However we have observed positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

Based on literature, liver does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-06-04

Question

I am looking for to test anti-mTOR/Tor Rabbit Monoclonal antibody M00003 on mouse cerebellum for research purposes, then I may be interested in using anti-mTOR/Tor Rabbit Monoclonal antibody M00003 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Brown

Verified customer

Asked: 2019-05-24

Answer

The products we sell, including anti-mTOR/Tor Rabbit Monoclonal antibody M00003, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-24

Question

Is this M00003 anti-mTOR/Tor Rabbit Monoclonal antibody reactive to the isotypes of MTOR?

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

The immunogen of M00003 anti-mTOR/Tor Rabbit Monoclonal antibody is A synthesized peptide derived from human mTOR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-15

Question

We are currently using anti-mTOR/Tor Rabbit Monoclonal antibody M00003 for rat tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

V. Baker

Verified customer

Asked: 2018-04-12

Answer

The anti-mTOR/Tor Rabbit Monoclonal antibody (M00003) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-mTOR/Tor Rabbit Monoclonal antibody M00003. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-11

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-11

Question

Our lab used your anti-mTOR/Tor Rabbit Monoclonal antibody for IF on testis in the past. I am using mouse, and We want to use the antibody for ICC next. Our lab want to know about examining testis as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for ICC?

A. Huang

Verified customer

Asked: 2017-01-23

Answer

I have checked the website and datasheets of our anti-mTOR/Tor Rabbit Monoclonal antibody and I see that M00003 has been validated on mouse in both IF and ICC. Thus M00003 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-01-23

Question

Please see the WB image, lot number and protocol we used for cerebellum using anti-mTOR/Tor Rabbit Monoclonal antibody M00003. Please let me know if you require anything else.

L. Collins

Verified customer

Asked: 2016-09-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-09-15

Question

Can you help my question with product M00003, anti-mTOR/Tor Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Mangal

Verified customer

Asked: 2013-05-01

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00003 anti-mTOR/Tor Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-05-01

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M00003
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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