Data & Images
|Product Name||Anti-TORC2 Antibody|
|Description||Rabbit IgG polyclonal antibody for CREB-regulated transcription coactivator 2(CRTC2) detection. Tested with WB in Human.|
|Cite This Product||Anti-TORC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1988)|
|Replacement Item||This antibody may replace the following items: sc-166445|sc-166445-X|sc-271912|sc-271912-X|sc-390765|sc-390765-X|sc-46272|sc-46272-X|sc-46273|sc-46273-X|sc-46274|sc-46274-X|sc-67147|sc-67147-X from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TORC2(595-611aa QDPHTFNHQNLTHCSRH), different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||CREB-regulated transcription coactivator 2|
|Molecular Weight||73302 MW|
|Protein Function||Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). .|
|Tissue Specificity||Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas. .|
|Sequence Similarities||Belongs to the TORC family.|
|Subcellular Localization||Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.|
|Alternative Names||CREB-regulated transcription coactivator 2;Transducer of regulated cAMP response element-binding protein 2;TORC-2;Transducer of CREB protein 2;CRTC2;TORC2;|
|Research Areas|||signal transduction|metabolism|energy metabolism| signal transduction|signaling pathway|nuclear signaling|nuclear hormone receptors|co-activators/co-repressors| neuroscience|neurology process| epigenetics and nuclear signaling|nuclear signaling pathways|nuclear receptors| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|types of disease|cancer||
Background for CREB-regulated transcription coactivator 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TORC2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TORC2 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human CRTC2, 29.3KD (162aa tag+ L585-Q693)
Lane 1: Recombinant Human CRTC2 Protein 10ng
Lane 2: Recombinant Human CRTC2 Protein 5ng
Lane 3: Recombinant Human CRTC2 Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,