Anti-TORC2/CRTC2 Antibody

Product Name

Anti-TORC2/CRTC2 Antibody

View all TORC2 Antibodies

SKU/Catalog Number

PA1988

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TORC2/CRTC2 Antibody catalog # PA1988. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TORC2/CRTC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1988)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TORC2, different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1988 is reactive to Crtc2 in Human

Applications

PA1988 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

73.216kDa

Background of TORC2

CREB regulated transcription coactivator 2, also known as CRTC2, is a protein which in humans is encoded by the CRTC2 gene. This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CRTC2 using anti-CRTC2 antibody (PA1988).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRTC2 antigen affinity purified polyclonal antibody (Catalog # PA1988) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRTC2 at approximately 80 kDa. The expected band size for CRTC2 is at 73 kDa.

Gene/Protein Information For Crtc2 (Source: Uniprot.org, NCBI)

Alternative Names

CREB regulated transcription coactivator 2; CREB-regulated transcription coactivator 2; CRTC2; TORC2; TORC-2; TORC2transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein 2 Crtc2|4632407F12Rik, Torc2|CREB regulated transcription coactivator 2|CREB-regulated transcription coactivator 2|TORC-2|transducer of CREB protein 2|transducer of regulated cAMP response element-binding protein 2

For more info on Crtc2, check out the Crtc2 Infographic

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In this infographic, you will see the following information for Crtc2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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