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Product Info Summary
| SKU: | PA1050 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Pig, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Myelin Basic Protein/MBP Antibody Picoband®
SKU/Catalog Number
PA1050
BA0094 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myelin Basic Protein/MBP Antibody catalog # PA1050. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Pig. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myelin Basic Protein/MBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1050)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1050 is reactive to MBP in Human, Mouse, Pig, Rat
Observed Molecular Weight
15-22 kDa
Calculated molecular weight
33.1 kDa
Background of MBP
Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1050 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, Pig
Immunofluorescence, 5-10μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue
IF: human brain tissue, mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of MBP using anti-MBP antibody (PA1050).
Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBP antigen affinity purified polyclonal antibody (PA1050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MBP at approximately 15-22 kDa. The expected band size for MBP is at 33 kDa.
Click image to see more details
IHC analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (PA1050).
Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myelin basic protein/MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IF analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
IF analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
IF analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
IF analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. HRP Conjugated Goat Anti-Rabbit IgG (BA1054) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 570 reagent at 1:200 dilution at room temperature for 10 minutes. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 570 and DAPI.
Click image to see more details
IF analysis of MBP using anti-MBP antibody ( PA1050).
ACAD9 was detected in a Frozen section of mouse spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 3% BSA. The tissue section was then incubated with 1:100 rabbit anti-MBP Antibody (PA1050) overnight at 4°C. CY3-conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a laser focol.
Click image to see more details
IF analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 10 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
BBR efficacy in reducing glial activation and the secretion of inflammatory cytokines in the hippocampus following anaesthesia and surgical interventions. (A) Utilises immunofluorescence staining to measure expression levels of GFAP across hippocampal regions CA1, CA3 and dentate gyrus (DG). Magnification: 400×. Scale bar = 50 μm. (B) Quantification of the GFAP‐positive fluorescence intensity in CA1, CA3 and DG areas. (C, D) Display the results of western blot analyses detailing the relative concentrations of TNF‐α and IL‐1β proteins in the hippocampus. Statistical values are expressed as mean ± SD, n = 3, significance indicated by * p < 0.05, ** p < 0.01.
Index in PubMed under a CC BY license. PMID: 40735856
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Post-treatment mRNA expression levels of MBP, CNP, and IL-1β in PFC. A Post-treatment mRNA level of MBP with qRT-PCR; B . Post-treatment mRNA level of CNP with qRT-PCR; C . Post-treatment mRNA level of IL-1β with qRT-PCR; Significant differences were revealed with * P < 0.05, ** P < 0.01, *** P < 0.001, n = 6 Full size image
Index in PubMed under a CC BY license. PMID: 40500721
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Post-treatment protein expression levels of MBP, CNP, and IL-1βwith western-blot in PFC. A Western blot bands of MBP, CNP, and IL-1βin PFC; B . Quantitative Analysis of MBP Protein by western blot; C . Quantitative Analysis of IL-1β Protein by western blot; D . Quantitative Analysis of CNP Protein by western blot. Significant differences were revealed with * P < 0.05, ** P < 0.01, *** P < 0.001, n = 6 Full size image
Index in PubMed under a CC BY license. PMID: 40500721
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Post-treatment immunostaining intensity of MBP protein with immunohistochemistry in medial PFC. A MBP protein in the control group; B . MBP protein in the CUS group; C . MBP protein in the CUS + FLU group; D . MBP protein in the CUS + EEgroup; E . MBP protein in the CUS + FLU + EE group. F . Immunohistochemical Quantification of MBP Protein in medial PFC. All scale bars represent 50 μm; “→”: positive staining in representative medial PFC. Significant differences were revealed with * P < 0.05, ** P < 0.01, n = 6 Full size image
Index in PubMed under a CC BY license. PMID: 40500721
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Thrombin receptor antagonist peptide promoted remyelination and neural functional recovery after subarachnoid hemorrhage (SAH). (A,B) Representative images of transmission electron microscopy and myelin basic protein (MBP) expression at 3 days after SAH are shown. Scale bar = 20 µm. (C) Respective myelin g -ratios, n = 125 for each group. (D) Respective modified neurological severity scale (mNSS) scores in each group at 1, 3, 5, 7, 9, 12, and 14 days after SAH, n = 10 for each group. The data are presented as the mean ± SEM. * P < 0.05 versus SAH + vehicle group, *** P < 0.001 versus SAH + vehicle group. n.s. indicates no significance.
Index in PubMed under a CC BY license. PMID: 29922213
Specific Publications For Anti-Myelin Basic Protein/MBP Antibody Picoband® (PA1050)
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Customer Reviews
Have you used Anti-Myelin Basic Protein/MBP Antibody Picoband®?
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1 Reviews For Anti-Myelin Basic Protein/MBP Antibody Picoband®
BOSTER’s rabbit anti-MBP antibody (catalog BA0094) exhibits high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.
Excellent
| SKU | PA1050 |
|---|---|
| Application | Immunofluorenscence |
| Sample | Mouse spinal cord |
| Sample Processing Description | Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat. |
| Primary Antibody | Anti-MBP9 antibody |
| Primary Incubation | 1:100, overnight at 4 ℃ |
| Blocking Agent | 3% BSA |
| Secondary Antibody | Anti-rabbit IgG-CY3 conjugated antibody. |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan). |
| Results Summary | BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study. |
Zhixiong Liu, Xiamen University
Verified customer
Submitted 2025-10-17
Customer Q&As
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17 Customer Q&As for Anti-Myelin Basic Protein/MBP Antibody Picoband®
Question
We have observed staining in mouse amygdala. Any tips? Is anti-Myelin Basic Protein/MBP antibody supposed to stain amygdala positively?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
From what I have seen in literature amygdala does express MBP. From what I have seen in Uniprot.org, MBP is expressed in c1 segment of cervical spinal cord, embryonic spinal cord, brain, amygdala, hippocampus subthalamic nucleus, brain, lung skin, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2020-04-16
Question
Will anti-Myelin Basic Protein/MBP antibody PA1050 work on goat IF with amygdala?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Our lab technicians have not tested anti-Myelin Basic Protein/MBP antibody PA1050 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Myelin Basic Protein/MBP antibody PA1050 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat amygdala in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-06
Question
Our lab want to know about using your anti-Myelin Basic Protein/MBP antibody for myelination studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
R. Yang
Verified customer
Asked: 2020-03-16
Answer
Thanks for your inquiry. This PA1050 anti-Myelin Basic Protein/MBP antibody is tested on mouse brain, brain tissue, rat brain tissue. It is guaranteed to work for IF, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-16
Question
I was wanting to use your anti-Myelin Basic Protein/MBP antibody for IHC-P for rat lung skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lung skin identification?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
You can see on the product datasheet, PA1050 anti-Myelin Basic Protein/MBP antibody has been tested for IF, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-27
Question
We ordered your anti-Myelin Basic Protein/MBP antibody for WB on hippocampus subthalamic nucleus in the past. I am using human, and We want to use the antibody for IHC-P next. I would like examining hippocampus subthalamic nucleus as well as amygdala in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
I looked at the website and datasheets of our anti-Myelin Basic Protein/MBP antibody and I see that PA1050 has been validated on human in both WB and IHC-P. Thus PA1050 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-26
Question
I am interested in to test anti-Myelin Basic Protein/MBP antibody PA1050 on rat lung skin for research purposes, then I may be interested in using anti-Myelin Basic Protein/MBP antibody PA1050 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The products we sell, including anti-Myelin Basic Protein/MBP antibody PA1050, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-18
Question
Will anti-Myelin Basic Protein/MBP antibody PA1050 work for IHC-P with lung skin?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
According to the expression profile of lung skin, MBP is highly expressed in lung skin. So, it is likely that anti-Myelin Basic Protein/MBP antibody PA1050 will work for IHC-P with lung skin.
Boster Scientific Support
Answered: 2019-06-26
Question
Our team were happy with the WB result of your anti-Myelin Basic Protein/MBP antibody. However we have been able to see positive staining in hippocampus subthalamic nucleus myelin membrane using this antibody. Is that expected? Could you tell me where is MBP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Based on literature, hippocampus subthalamic nucleus does express MBP. Generally MBP expresses in myelin membrane. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-06-17
Question
Is a blocking peptide available for product anti-Myelin Basic Protein/MBP antibody (PA1050)?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
We do provide the blocking peptide for product anti-Myelin Basic Protein/MBP antibody (PA1050). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-09
Question
Is this PA1050 anti-Myelin Basic Protein/MBP antibody reactive to the isotypes of MBP?
C. Walker
Verified customer
Asked: 2018-05-11
Answer
The immunogen of PA1050 anti-Myelin Basic Protein/MBP antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein(182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-11
Question
Do you have a BSA free version of anti-Myelin Basic Protein/MBP antibody PA1050 available?
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Myelin Basic Protein/MBP antibody PA1050 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung skin using anti-Myelin Basic Protein/MBP antibody PA1050. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-15
Question
We are currently using anti-Myelin Basic Protein/MBP antibody PA1050 for rat tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
D. Yang
Verified customer
Asked: 2017-10-12
Answer
The anti-Myelin Basic Protein/MBP antibody (PA1050) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-12
Question
My question regarding product PA1050, anti-Myelin Basic Protein/MBP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Moore
Verified customer
Asked: 2017-06-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1050 anti-Myelin Basic Protein/MBP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-09
Question
I see that the anti-Myelin Basic Protein/MBP antibody PA1050 works with IHC-P, what is the protocol used to produce the result images on the product page?
P. Wu
Verified customer
Asked: 2016-09-14
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-09-14
Question
See attached the WB image, lot number and protocol we used for lung skin using anti-Myelin Basic Protein/MBP antibody PA1050. Please let me know if you require anything else.
E. Li
Verified customer
Asked: 2014-09-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-25
Question
Would PA1050 anti-Myelin Basic Protein/MBP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Taylor
Verified customer
Asked: 2014-02-04
Answer
As indicated on the product datasheet, PA1050 anti-Myelin Basic Protein/MBP antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-04
