HMOX1 antibody (PB9212) performs well in IHC, WB and shows reactivity with Human.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 6 Publications
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Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®

HMOX1 antibody

Reactive SpeciesHostApplication
HumanRabbitIHC, WB

SKU: PB9212

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

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Quantity: 1

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Guarantee / Product Promise

Tested Applications

HMOX1 antibody was positively detected by WB in:

human A549 whole cell, human Hela whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

HMOX1 antibody was positively detected by IHC in:

human tonsil tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®
View all HMOX1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HMOX1 recombinant protein (Position: M1-M288). Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated Molecular Weight

32.8 kDa

Background of HMOX1
HMOX1 (heme oxygenase (decycling) 1), also known as HO-1, is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.
Cite This Product
Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9212)

Protocols

Western Blot protocol for HMOX1 antibody
Immunohistochemistry (IHC) protocol for HMOX1 antibody

Publications

Application:
Species:
Fucoxanthin regulates Nrf2 signaling to decrease oxidative stress and improves renal fibrosis depending on Sirt1 in HG-induced GMCs and STZ-induced diabetic rats
Published: 2021-11-12
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.43
Species: Rat
PMID: 34780751
Nifedipine inhibits oxidative stress and ameliorates osteoarthritis by activating the nuclear factor erythroid-2-related factor 2 pathway
Published: 2020-01-09
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.65
Species: Human
PMID: 31927051
Structural and antioxidant analysis of Tartary buckwheat (Fagopyrum tartaricum Gaertn.) 13S globulin
Published: 2019-11-04
Journal: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Application: WB
Impact Factor: 2.42
Species: Human
PMID: 31680256
Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro
Published: 2019-10-28
Journal: CELL BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 2.32
Species: Human
PMID: 31659617
Myricetin ameliorates brain injury and neurological deficits via Nrf2 activation after experimental stroke in middle-aged rats
Published: 2016-04-28
Journal: Food & Function
Application: WB
Impact Factor: 2.69
Species: Rat
PMID: 27171848
Heme oxygenase-1 expression in rats with acute lung rejection and implication
Published: 2009-02-18
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: IHC,WB
Impact Factor: 0.00
Species: Rat
PMID: 19224170

Review

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1 Review For Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®

The signal was very strong and clean!--Chi Li, MEDICINE, UNIVERSITY OF LOUISVILLE, PROFESSOR

Source: Biocompare.com

SKU PB9212
Applications Western Blot
Sample Whole cell extract of pancreatic tumor cell MIA PaACa2
Detection Enhanced chemiluminescence

"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."

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Product Promise

We promise Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PB9212
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 6, 2026