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HMOX1 antibody (PB9212) performs well in IHC, WB and shows reactivity with Human.
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
HMOX1 antibody was positively detected by WB in:
human A549 whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HMOX1 antibody was positively detected by IHC in:
human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
32.8 kDa
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Source: Biocompare.com
| SKU | PB9212 |
|---|---|
| Applications | Western Blot |
| Sample | Whole cell extract of pancreatic tumor cell MIA PaACa2 |
| Detection | Enhanced chemiluminescence |
"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."
Chi Li, University of Louisville
Verified customer
Submitted 2016-10-20
We promise Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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This product is for Research Use Only. Not for use in diagnostic procedures.