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RELA antibody (A00284-1) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 143 Publications

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a00284 1 rela primary antibodies fcm testing 4

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Flow Cytometry analysis of Hela cells using anti-NF-kB p65 antibody (A00284-1).
Overlay histogram showing Hela cells stained with A00284-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF-kB p65 Antibody (A00284-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Designing a Western blot for RELA? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the RELA Western Blot Guide

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Anti-NF-kB p65/RELA Antibody Picoband®

Name: Anti-NF-kB p65/RELA Antibody Picoband®
Brand: Boster Bio
SKU: A00284-1
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

RELA antibody

143 Publications|

2 Reviews

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, ICC, WB

Tested Applications

RELA antibody was positively detected by WB in:

human Hela whole cell lysates, human Raji whole cell lysates, human HepG2 whole cell lysates, human K562 whole cell lysates, rat PC-12 whole cell lysates, rat RH35 whole cell lysates, mouse RAW264.7 whole cell lysates, mouse HEPA1/6 whole cell lysates, human HAP1-WT whole cell, human HAP1-NF-kB p65 KO whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

RELA antibody was positively detected by ICC/IF in:

U2OS cell

RELA antibody was positively detected by IP in:

HepG2

RELA antibody was positively detected by FC in:

Hela cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Product Information

Product Name

Anti-NF-kB p65/RELA Antibody Picoband®
View all RELA Antibodies

Form

Lyophilized

Uniprot

Q04206

Gene ID

5970

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human NF-kB p65 recombinant protein (Position: F99-S551).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

65-70 kDa

Calculated Molecular Weight

60.2 kDa

Background of RELA

Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein that in humans is encoded by the RELA gene. It is mapped to 11q13.1. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.

Cite This Product

Anti-NF-kB p65/RELA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00284-1)

Protocols

Western Blot protocol for RELA antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RELA antibody

Review Protocol

Immunoprecipitation (IP) protocol for RELA antibody

Review Protocol

Flow Cytometry (FC) protocol for RELA antibody

Review Protocol

Publications

Inactivation method-dependent differences in Lactiplantibacillus plantarum dy-1 postbiotics: heat treatment enhances fermentability and macrophage-modulating potential

Published: 2026-08-31

Journal: Food Bioscience

Application: WB

Impact Factor: 6.20

Species: Mouse

SKU	A00284-1
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	① Normal mouse hippocampal tissue, ② Hippocampal tissue from Alzheimer’s disease model mouse, ③ Hippocampal tissue from Alzheimer’s disease model mouse treated with a self-developed drug. Total protein was extracted from all samples.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	NF-kB p65/RELA Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	NF-κB p65 (RelA) is one of the most classical and essential subunits of the NF-κB transcription factor family. In Alzheimer’s disease, activation of p65 can initiate neuroprotective programs in neurons. The results showed that p65 expression was significantly increased in the brains of Alzheimer’s disease model mice and decreased after treatment, consistent with the expected outcomes.

SKU	A00284-1
Application	Immunofluorenscence
Sample	Mouse cell climbing slides
Sample Processing Description	Cells were directly lysed in NP40 lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. The samples were then loaded onto SDS-PAGE, with 20 μl of cell protein sample per lane.
Primary Antibody	Anti-NF-kB p65/RELA Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Blocking Agent	5% Non-fat milk
Secondary Antibody	HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Imaging system: Tanon, ECL substrate
Results Summary	I will purchase Boster products again and recommend them to my classmates and colleagues.

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Anti-NF-kB p65/RELA Antibody Picoband®

RELA antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Inactivation method-dependent differences in Lactiplantibacillus plantarum dy-1 postbiotics: heat treatment enhances fermentability and macrophage-modulating potential

Bioinformatic identification of THY1 as a target in osteoarthritis and its effects on chondrocyte senescence and apoptosis

Distinct roles of the Anopheles gambiae Imd/Rel2 and Toll/Rel1 pathways in resistance to systemic bacterial and fungal infections

Mechanisms of Mulberry Leaf Extracts Ameliorating Inflammation and Fibrosis in Non-Alcoholic Fatty Liver Disease Mice via NF-κB and TGFβ1/Smad3 Pathways

Using the Phillygenin Ameliorates the Severe Acute Pancreatitis in Rats by Inhibiting TLR4/NF-κB Pathway

Anti-asthmatic effects and ingredient annotation of the active fraction from Ganoderma lucidum

Antimicrobial Peptide pBD2 Alleviates Intestinal Damage by Regulating MAPK and Nrf2 Signaling Pathway and Intestinal Microbiota in Mice Challenged with Enterotoxigenic Escherichia Coli K88

In Vitro and In Vivo Validation of Endothelium-Derived Potential Therapeutics for Myocardial Ischemia/Reperfusion Injury Identified by an AI-Enhanced Single-Cell and Virtual-Cell Paradigm

A novel polysaccharide from Polygonatum cyrtonema fibrous root mediates anti-diarrheal effects by reprogramming macrophage M1/M2 polarization

Occupational silica exposure drives systemic immune dysregulation and tumor microenvironment susceptibility: evidence from a real-world study

Bacillus amyloliquefaciens Fmb50 Alleviates Hyperglycemia by Reshaping Gut Structures and Mitigating Metabolic Disorders in Type 2 Diabetic Mice

LINC01123 drives autophagy-mediated promotion of ovarian tumor metastasis through the p65/ATG13 pathway

TWEAK exacerbates allergic conjunctivitis via PKM2-dependent NF-κB activation and macrophage M1 polarization

Ustiloxins induce kidney injury via the TLR2/MAPK/NF-κB pathway

Acute Normovolemic Hemodilution Changes the Aquaporin Expression Profile in Specific Tissues and Induces Apoptotic and Inflammatory Processes in a Rat Model

High intake of n-6 polyunsaturated fatty acid exacerbates non-alcoholic steatohepatitis by the involvement of multiple metabolic pathways

Anti-inflammatory effects of apigenin on LPS-induced mastitis in lactating SD rats through inhibiting TLR4/NF-κB signaling pathway

Ginsenosides Modulate Immunity via TLR4/MyD88/NF-κB Pathway and Gut Microbiota

A vascular endothelial cell, neuron, and microglia tri-culture model to study hypertension-related depression

Aloperine treatment attenuates acute spinal cord injury by reducing oxidative, inflammatory, and apoptotic responses via PI3K/AKT/NF-κB signaling in a rat contusion model

Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction

Temporal and spatial expression of Phosphodiesterase-4B after sciatic nerve compression in rats and its mechanism of action on sciatic nerve repair

Protective effect of valsartan alone and in combination with neprilysin inhibitor (valsartan + sacubitril) against hepatic ischemia–reperfusion injury: targeting angiotensin II receptor-neprilysin and modulating SMAD-4/NF-κβ/JNK pathways in rats

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

The H4R antagonist, JNJ-7777120 treatments ameliorate mild traumatic brain injury by reducing oxidative damage, inflammatory and apoptotic responses through blockage of the ERK1/2/NF-κB pathway in a rat model

Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro

Folic Acid Attenuates N-Methyl-N’-Nitro-N-Nitrosoguanidine-Induced Gastric Mucosal Injury in Rats

Anti-inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF-κB and MAPK signaling pathways

Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis.

Cerium oxide nanoparticles (nanoceria) pretreatment attenuates cell death in the hippocampus and cognitive dysfunction due to repeated isoflurane anesthesia in newborn rats.

Overactivation of NF-kB pathway can induce apoptosis by down-regulating glycolysis in human degenerative nucleus pulposus cells

The Role of Grifola frondosa Polysaccharide in Preventing Skeletal Muscle Atrophy in Type 2 Diabetes Mellitus

Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Three Artemisia pollens trigger the onset of allergic rhinitis via TLR4/MyD88 signaling pathway

Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway

UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice

Bisphenol A exposure exacerbates tracheal inflammatory injury in selenium-deficient chickens by regulating the miR-155/TRAF3/ROS pathway

Hippophae rhamnoides reverses decreased CYP2D6 expression in rats with BCG-induced liver injury

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration

Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea)

Mechanism investigation of Duhuo Jisheng pill against rheumatoid arthritis based on a strategy for the integration of network pharmacology, molecular docking and in vivo experimental verification

Influence of Sodium New Houttuyfonate as a New EGFR-TK Inhibitor on the Apoptosis and Autophagy of MCF-7 Cells and Its Toxicity to Caenorhabditis elegans

Effect of STING signaling on intestinal barrier damage in severe acute pancreatitis

Istradefylline induces A2A/P2X7 crosstalk expression inducing pro-inflammatory signal, and reduces AKT/mTOR signaling in melanoma-bearing mice

Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells

Active ingredients screening and pharmacological mechanism research of curcumae rhizoma-sparganii rhizoma herb pair ameliorates liver fibrosis based on network pharmacology

Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells

Explore the mechanism and substance basis of Mahuang FuziXixin Decoction for the treatment of lung cancer based on network pharmacology and molecular docking

Pirfenidone attenuates nonalcoholic fatty liver disease through activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway

Radiation-induced NF-κB activation is involved in cochlear damage in mice via promotion of a local inflammatory response

Acutely increased aquaporin-4 exhibits more potent protective effects in the cortex against single and repeated isoflurane-induced neurotoxicity in the developing rat brain

An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties

Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling

Human Neural Stem Cell Secretome Inhibits Lipopolysaccharide-Induced Neuroinflammation Through Modulating Microglia Polarization by Activating Peroxisome Proliferator-Activated Receptor Gamma

Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway

Intravenous immunoglobulin is effective in alleviating hepatic ischemia–reperfusion injury: a rat model study

The antimicrobial peptide YD attenuates inflammation via miR-155 targeting CASP12 during liver fibrosis

Liuweidihuang Pill Alleviates Inflammation of the Testis via AMPK/SIRT1/NF-κB Pathway in Aging Rats

HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway

Retracted Article: The PI3K/Akt and NF-κB signaling pathways are involved in the protective effects of Lithocarpus polystachyus (sweet tea) on APAP-induced oxidative stress injury in mice

Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers

LncRNA NEAT1 promotes the progression of sepsis-induced myocardial cell injury by sponging miR-144-3p

Partial liquid ventilation–induced mild hypothermia improves the lung function and alleviates the inflammatory response during acute respiratory distress syndrome in canines

PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-B Signaling Pathway

Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells

Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll‑like receptor‑4/nuclear factor‑κB signaling pathway

The effects of genistein and puerarin on the activation of nuclear factor-KB and the production of tumor necrosis factor-α in asthma patients