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Product Info Summary
| SKU: | PB9324 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NF-kB p65/RELA Antibody Picoband®
SKU/Catalog Number
PB9324
PB0321 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NF-kB p65/RELA Antibody Picoband® catalog # PB9324. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-kB p65/RELA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9324)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse NF-kB p65 recombinant protein (Position: D291-Q479). Mouse NF-kB p65 shares 77% amino acid (aa) sequence identity with human NF-kB p65.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9324 is reactive to Rela in Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
60.2 kDa
Background of Rela
Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein encoded by the RELA gene. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9324 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: mouse NIH/3T3 whole cell, mouse ANA-1 whole cellrat testis tissuerat PC-12 whole cell
Validation Images & Assay Conditions
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Molecular docking diagram. (A) HBOA-MAPK (B) HBOA-FoXO, (C) HBOA-AMPK, (D) HBOA-JAK, (E) HBOA-STAT, (F) HBOA-NF-κB, (G) HBOA-PPAR, (H) HBOA-Ras, and (I) HBOA-TLR4. (J) Binding energy score diagram obtained by molecular docking.
Index in PubMed under a CC BY license. PMID: 39323644
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HBOA inhibits the TLR4/NF-κB signaling pathway. The protein expressions of (A) TLR4 and MyD88, (B) p-IKKα/β, IKKα/β, p-IκBα, IκBα, p-NF-κBp65, and NF-κBp65, and (C) nucleus NF-κBp65, Lamin B1, and cytosol NF-κBp65 were examined by Western blotting. * P < 0.05, ** P < 0.01 versus the model group; ## P < 0.01 versus the normal group.
Index in PubMed under a CC BY license. PMID: 39323644
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Integrated analysis of transcriptomics and metabolomics. (A) Correlation heat map. (B) 9-quadrant map. (C) Bubble plot of the Joint-Pathway analysis of differential metabolites and differential genes. (D) Integration of metabolite and gene relationships in the glycerophospholipid metabolic pathway. (E–I) The contents of Glycerophospholipid metabolism pathway-related metabolites and the expression levels of the target genes.
Index in PubMed under a CC BY license. PMID: 39323644
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Western blot analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PB9324).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates,
Lane 2: mouse ANA-1 whole cell lysates.
Lane 3: rat testis tissue lysates.
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65/RELA antigen affinity purified polyclonal antibody (Catalog # PB9324) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65/RELA at approximately 65 kDa. The expected band size for NF-kB p65/RELA is at 60 kDa.
Specific Publications For Anti-NF-kB p65/RELA Antibody Picoband® (PB9324)
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1 Customer Q&As for Anti-NF-kB p65/RELA Antibody Picoband®
Question
We are currently using anti-NF-kB p65/RELA antibody PB9324 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on pig tissues as well?
J. Li
Verified customer
Asked: 2014-04-21
Answer
The anti-NF-kB p65/RELA antibody (PB9324) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-21
