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Product Info Summary
| SKU: | M02496-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-NTAL Purified Lat2 Monoclonal Antibody
SKU/Catalog Number
M02496-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NTAL Purified Lat2 Monoclonal Antibody (Catalog# M02496-1). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-NTAL Purified Lat2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02496-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
NAP-07
Isotype
Mouse IgG1
Immunogen
Recombinant cytoplasmic domain (aa 91-243) of human NTAL. The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02496-1 is reactive to Lat2 in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
22.9 kDa
Background of Lat2
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02496-1 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml.
Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.
Validation Images & Assay Conditions
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Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).
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Separation of human CD3 negative NTAL positive lymphocytes (red-filled) from CD3 positive NTAL negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 µg/ml, GAM APC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 µg/ml, GAM APC).
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Immunocytochemistry analysis of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. The cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).
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Western blotting analysis of human NTAL using mouse monoclonal antibody NAP-07 on lysates of Raji and Ramos cells and on Jurkat cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NTAL was detected around 27 kDa.
Specific Publications For Anti-NTAL Purified Lat2 Monoclonal Antibody (M02496-1)
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