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Product Info Summary
| SKU: | A10117-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-HNK-1ST CHST10 Antibody
SKU/Catalog Number
A10117-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HNK-1ST CHST10 Antibody catalog # A10117-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNK-1ST CHST10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10117-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CHST10. AA range:191-240
Reactive Species
A10117-1 is reactive to CHST10 in Human, Mouse, Rat
Calculated molecular weight
42.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10117-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using HNK-1ST Polyclonal Antibody
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Western blot analysis of lysates from HUVEC cells, using CHST10 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CHST10 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-HNK-1ST CHST10 Antibody (A10117-1)
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Customer Q&As
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4 Customer Q&As for Anti-HNK-1ST CHST10 Antibody
Question
Is there a BSA free version of anti-HNK-1ST antibody A10117-1 available?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HNK-1ST antibody A10117-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-08
Question
Is a blocking peptide available for product anti-HNK-1ST antibody (A10117-1)?
J. Thomas
Verified customer
Asked: 2018-11-21
Answer
We do provide the blocking peptide for product anti-HNK-1ST antibody (A10117-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-11-21
Question
Will anti-HNK-1ST antibody A10117-1 work on goat WB with brain?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
Our lab technicians have not validated anti-HNK-1ST antibody A10117-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-HNK-1ST antibody A10117-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-26
Question
Is this A10117-1 anti-HNK-1ST antibody reactive to the isotypes of CHST10?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
The immunogen of A10117-1 anti-HNK-1ST antibody is Synthesized peptide derived from the Internal region of human HNK-1ST. at AA rangle: 160-240. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-18
