Anti-PARP PARP1 Rabbit Monoclonal Antibody

PARP1 antibody

Boster Bio Anti-PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M00122
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-PARP PARP1 Rabbit Monoclonal Antibody

View all PARP1 Antibodies

SKU/Catalog Number

M00122

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PARP PARP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00122)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABCG-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PARP

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00122 is reactive to PARP1 in Human, Mouse, Rat

Applications

M00122 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

113.084kDa

Background of PARP1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:100-1:500
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For PARP1 (Source: Uniprot.org, NCBI)

Gene Name

PARP1

Full Name

Poly [ADP-ribose] polymerase 1

Weight

113.084kDa

Alternative Names

ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1 PARP1 ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1 poly(ADP-ribose) polymerase 1 poly [ADP-ribose] polymerase 1|ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|ADP-ribosyltransferase diphtheria toxin-like 1|DNA ADP-ribosyltransferase PARP1|NAD(+) ADP-ribosyltransferase 1|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase|poly[ADP-ribose] synthase 1|protein poly-ADP-ribosyltransferase PARP1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PARP1, check out the PARP1 Infographic

PARP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PARP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00122 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
M00122 usage in article: APP:WB, SAMPLE:A431 CELLS, DILUTION:NA

Li Q, Li Z, Xu Xy, Guo Yl, Du F. Int J Mol Sci. 2010 Nov 16;11(11):4580-90. Doi: 10.3390/Ijms11114580. Neuroprotective Properties Of Picroside Ii In A Rat Model Of Focal Cerebral Ischemia.

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16 Customer Q&As for Anti-PARP PARP1 Rabbit Monoclonal Antibody

Question

I see that the anti-PARP Rabbit Monoclonal antibody M00122 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-30

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PARP Rabbit Monoclonal antibody M00122. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

Does M00122 anti-PARP Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

As indicated on the product datasheet, M00122 anti-PARP Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-10

Question

Is a blocking peptide available for product anti-PARP Rabbit Monoclonal antibody (M00122)?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

We do provide the blocking peptide for product anti-PARP Rabbit Monoclonal antibody (M00122). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-24

Question

Is this M00122 anti-PARP Rabbit Monoclonal antibody reactive to the isotypes of PARP1?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

The immunogen of M00122 anti-PARP Rabbit Monoclonal antibody is A synthesized peptide derived from human PARP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-14

Question

Will anti-PARP Rabbit Monoclonal antibody M00122 work for ICC with liver?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

According to the expression profile of liver, PARP1 is highly expressed in liver. So, it is likely that anti-PARP Rabbit Monoclonal antibody M00122 will work for ICC with liver.

Boster Scientific Support

Answered: 2019-09-11

Question

I am looking for to test anti-PARP Rabbit Monoclonal antibody M00122 on rat liver for research purposes, then I may be interested in using anti-PARP Rabbit Monoclonal antibody M00122 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

The products we sell, including anti-PARP Rabbit Monoclonal antibody M00122, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-21

Question

My colleagues were happy with the WB result of your anti-PARP Rabbit Monoclonal antibody. However we have observed positive staining in liver nucleus using this antibody. Is that expected? Could you tell me where is PARP1 supposed to be expressed?

C. Gonzalez

Verified customer

Asked: 2019-07-23

Answer

According to literature, liver does express PARP1. Generally PARP1 expresses in nucleus. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-07-23

Question

We are currently using anti-PARP Rabbit Monoclonal antibody M00122 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

The anti-PARP Rabbit Monoclonal antibody (M00122) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-07

Question

Our lab used your anti-PARP Rabbit Monoclonal antibody for WB on leukemic t-cell in a previous project. I am using rat, and We are going to use the antibody for IHC next. I am looking for examining leukemic t-cell as well as colon carcinoma ovarian carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-05-23

Answer

I took a look at the website and datasheets of our anti-PARP Rabbit Monoclonal antibody and it seems that M00122 has been tested on rat in both WB and IHC. Thus M00122 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-23

Question

My question regarding product M00122, anti-PARP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Carter

Verified customer

Asked: 2018-04-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00122 anti-PARP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-04-19

Question

I am looking for using your anti-PARP Rabbit Monoclonal antibody for mitochondrial dna repair studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-04-09

Answer

Thanks for your inquiry. This M00122 anti-PARP Rabbit Monoclonal antibody is tested on hela cells. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-04-09

Question

We have observed staining in mouse liver. Are there any suggestions? Is anti-PARP Rabbit Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2018-03-02

Answer

According to literature liver does express PARP1. According to Uniprot.org, PARP1 is expressed in heart left ventricle, fibroblast, brain, colon carcinoma ovarian carcinoma, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-03-02

Question

Is there a BSA free version of anti-PARP Rabbit Monoclonal antibody M00122 available?

Verified Customer

Verified customer

Asked: 2017-10-02

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PARP Rabbit Monoclonal antibody M00122 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-02

Question

I was wanting to use your anti-PARP Rabbit Monoclonal antibody for ICC for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?

Verified Customer

Verified customer

Asked: 2017-05-22

Answer

You can see on the product datasheet, M00122 anti-PARP Rabbit Monoclonal antibody has been tested for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-22

Question

I have attached the WB image, lot number and protocol we used for liver using anti-PARP Rabbit Monoclonal antibody M00122. Please let me know if you require anything else.

V. Li

Verified customer

Asked: 2014-06-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-06-03

Order DetailsPrice
M00122

100μL

$370
M00122-30ul

30μL sample

$99
M00122-carrier-free

Carrier Free

$370

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