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- Table of Contents
PCNA antibody (M00125-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 15 Publications
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Immunohistochemical analysis of paraffin-embedded human colon, using PCNA Antibody.
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Anti-PCNA Rabbit Monoclonal Antibody
PCNA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
PCNA antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human U-937 whole cell, human 293T whole cell, mouse spleen tissue, rat RAW2647 tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PCNA antibody was positively detected by IHC in:
human colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PCNA antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-PCNA Rabbit Monoclonal Antibody
View all PCNA Antibodies
View all PCNA Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PCNA
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
28.8 kDa
Cite This Product
Anti-PCNA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00125-1)
Protocols
Western Blot protocol for PCNA antibody
Immunohistochemistry (IHC) protocol for PCNA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PCNA antibody
Publications
Application:
Species:
Astragaloside IV targets TUBB4B to inhibit proliferation and promote apoptosis of pituitary tumor cells via the STMN1/ERK pathway
Published: 2026-04-01
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 5.80
Species: Rat
PMID: 41930558
Histone lactylation-derived prelamin A accelerates pulmonary arterial smooth muscle cells senescence in hypoxia-induced pulmonary hypertension rats
Published: 2025-05-20
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IF,WB
Impact Factor: 4.70
Species: Rat
PMID: 40398256
5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2
Published: 2025-01-10
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.50
Species: Human
PMID: 39800017
Tailoring Carrier-Free Nanoparticles based on Natural Small Molecule Assembly for Synergistic Anti-tumor Efficacy
Published: 2024-11-08
Journal: Asian Journal of Pharmaceutical Sciences
Application: IHC
Impact Factor: 10.70
Species: Mouse
Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis
Published: 2024-02-29
Journal: CELLULAR AND MOLECULAR BIOLOGY
Application: WB
Impact Factor: 1.50
Species: Human
PMID: 38430028
Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes
Published: 2024-02-17
Journal: BIOLOGICAL TRACE ELEMENT RESEARCH
Application: WB
Impact Factor: 3.40
Species: Rat
PMID: 38367173
Overcoming chemoresistance in non-angiogenic colorectal cancer by metformin via inhibiting endothelial apoptosis and vascular immaturity
Published: 2023-02-09
Journal: Journal of Pharmaceutical Analysis
Application: IF
Impact Factor: 8.80
Species: Mouse
PMID: 37102105
Prevotellaceae produces butyrate to alleviate PD-1/PD-L1 inhibitor-related cardiotoxicity via PPARα-CYP4X1 axis in colonic macrophages
Published: 2022-01-03
Journal: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
Application: IHC
Impact Factor: 12.66
Species: Mouse
PMID: 34980222
CCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells
Published: 2020-06-30
Journal: OncoTargets and Therapy
Application: WB
Impact Factor: 3.34
Species: Mouse,Human
PMID: 32669853
Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells
Published: 2019-12-17
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Human
PMID: 31974617
Biological characteristics of heat shock protein 90 in human liver cancer cells
Published: 2019-04-15
Journal: American Journal of Translational Research
Application: IF
Impact Factor: 3.27
Species: Human
PMID: 31105855
Dacomitinib, a new pan-EGFR inhibitor, is effective in attenuating pulmonary vascular remodeling and pulmonary hypertension
Published: 2019-02-10
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 3.17
Species: Rat
PMID: 30753867
Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1
Published: 2018-03-09
Journal: Oncology Letters
Application: WB
Impact Factor: 1.66
Species: Mouse
PMID: 29731875
The enhanced antitumor-specific immune response with mannose- and CpG-ODN-coated liposomes delivering TRP2 peptide
Published: 2018-02-12
Journal: Theranostics
Impact Factor: 8.54
PMID: 29556352
Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells
Published: 2014-02-04
Journal: REPRODUCTIVE TOXICOLOGY
Application: IF
Impact Factor: 2.77
Species: Mouse
PMID: 24503145
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Product Promise
We promise Anti-PCNA Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00125-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026