Click image to see more details
Product Info Summary
| SKU: | M01033 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Paxillin PXN Rabbit Monoclonal Antibody
SKU/Catalog Number
M01033
BM4153 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Paxillin PXN Rabbit Monoclonal Antibody catalog # M01033. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Paxillin PXN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01033)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BIF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Paxillin
Reactive Species
M01033 is reactive to PXN in Human, Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
64.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01033 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human K562 whole cell, human U2OS whole cell, rat heart tissue, mouse heart tissue
IHC: human breast carcinoma tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PXN using anti-PXN antibody (M01033).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXN antigen affinity purified monoclonal antibody (M01033) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PXN at approximately 65 kDa. The expected band size for PXN is at 65 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Paxillin Antibody.
Specific Publications For Anti-Paxillin PXN Rabbit Monoclonal Antibody (M01033)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Paxillin PXN Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Paxillin PXN Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
8 Customer Q&As for Anti-Paxillin PXN Rabbit Monoclonal Antibody
Question
Our lab used your anti-Paxillin Rabbit Monoclonal antibody for WB on fundus of stomach in a previous experiment. I am using human, and I plan to use the antibody for IP next. you antibody examining fundus of stomach as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
I have checked the website and datasheets of our anti-Paxillin Rabbit Monoclonal antibody and I see that M01033 has been validated on human in both WB and IP. Thus M01033 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-19
Question
you antibody using your anti-Paxillin Rabbit Monoclonal antibody for ras gtpase and map kinases studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Thank you for your inquiry. This M01033 anti-Paxillin Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-22
Question
We have observed staining in mouse placenta. Are there any suggestions? Is anti-Paxillin Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
From what I have seen in literature placenta does express PXN. From what I have seen in Uniprot.org, PXN is expressed in fundus of stomach, placenta, fetal kidney, brain testis, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2020-01-15
Question
My colleagues were satisfied with the WB result of your anti-Paxillin Rabbit Monoclonal antibody. However we have observed positive staining in liver cytoskeleton using this antibody. Is that expected? Could you tell me where is PXN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
From what I have seen in literature, liver does express PXN. Generally PXN expresses in cytoplasm, cytoskeleton. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2019-10-01
Question
Our lab want to know about to test anti-Paxillin Rabbit Monoclonal antibody M01033 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Paxillin Rabbit Monoclonal antibody M01033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The products we sell, including anti-Paxillin Rabbit Monoclonal antibody M01033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-13
Question
I was wanting to use your anti-Paxillin Rabbit Monoclonal antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
N. Singh
Verified customer
Asked: 2019-04-02
Answer
As indicated on the product datasheet, M01033 anti-Paxillin Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-02
Question
Is this M01033 anti-Paxillin Rabbit Monoclonal antibody reactive to the isotypes of PXN?
A. Carter
Verified customer
Asked: 2015-07-02
Answer
The immunogen of M01033 anti-Paxillin Rabbit Monoclonal antibody is A synthesized peptide derived from human Paxillin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-07-02
Question
We are currently using anti-Paxillin Rabbit Monoclonal antibody M01033 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
C. Edwards
Verified customer
Asked: 2013-06-18
Answer
The anti-Paxillin Rabbit Monoclonal antibody (M01033) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-18
