Anti-Phospho-Cdk9 (T186) Antibody

Boster Bio Anti-Phospho-Cdk9 (T186) Antibody catalog # P00794. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P00794
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-Cdk9 (T186) Antibody

See all CDK9 products

SKU/Catalog Number

P00794

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Cdk9 (T186) Antibody catalog # P00794. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Cdk9 (T186) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00794)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human Cdk9 around the phosphorylation site of T186.

Reactive Species

P00794 is reactive to CDK9 in Human, Mouse, Rat

Applications

P00794 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For CDK9 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50750

Gene ID

1025

Gene Name

CDK9

Full Name

Cyclin-dependent kinase 9

Weight

42.778kDa

Superfamily

protein kinase superfamily

Alternative Names

C-2k; CDC2L4CTK1; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); cyclin-dependent kinase 9; EC 2.7.11; EC 2.7.11.22; EC 2.7.11.23; PITALRE; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK CDK9 C-2k, CDC2L4, CTK1, PITALRE, TAK cyclin dependent kinase 9 cyclin-dependent kinase 9|CDC2-related kinase|cell division cycle 2-like protein kinase 4|cell division protein kinase 9|serine/threonine protein kinase PITALRE|tat-associated kinase complex catalytic subunit

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDK9, check out the CDK9 Infographic

CDK9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDK9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for P00794

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Phospho-Cdk9 (T186) Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Phospho-Cdk9 (T186) Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Phospho-Cdk9 (T186) Antibody

Question

Is there a BSA free version of anti-Phospho-Cdk9 (T186) antibody P00794 available?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Cdk9 (T186) antibody P00794 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-23

Question

I was wanting to use your anti-Phospho-Cdk9 (T186) antibody for WB for mouse right ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right ovary identification?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

As indicated on the product datasheet, P00794 anti-Phospho-Cdk9 (T186) antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse right ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-26

Question

We are interested in using your anti-Phospho-Cdk9 (T186) antibody for negative regulation of mrna polyadenylation studies. Has this antibody been tested with western blotting on 3t3 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

I appreciate your inquiry. This P00794 anti-Phospho-Cdk9 (T186) antibody is validated on 3t3 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-23

Question

Is this P00794 anti-Phospho-Cdk9 (T186) antibody reactive to the isotypes of CDK9?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

The immunogen of P00794 anti-Phospho-Cdk9 (T186) antibody is Synthesized peptide derived from human Cdk9 around the phosphorylation site of T186. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-23

Question

Here is the WB image, lot number and protocol we used for right ovary using anti-Phospho-Cdk9 (T186) antibody P00794. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-09

Question

Will P00794 anti-Phospho-Cdk9 (T186) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Dhar

Verified customer

Asked: 2019-08-19

Answer

You can see on the product datasheet, P00794 anti-Phospho-Cdk9 (T186) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-19

Question

We have been able to see staining in mouse right ovary. Are there any suggestions? Is anti-Phospho-Cdk9 (T186) antibody supposed to stain right ovary positively?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

According to literature right ovary does express CDK9. According to Uniprot.org, CDK9 is expressed in right ovary, cervix, cervix carcinoma, liver, among other tissues. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-08-06

Question

I see that the anti-Phospho-Cdk9 (T186) antibody P00794 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-23

Question

Is a blocking peptide available for product anti-Phospho-Cdk9 (T186) antibody (P00794)?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

We do provide the blocking peptide for product anti-Phospho-Cdk9 (T186) antibody (P00794). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-28

Question

We are currently using anti-Phospho-Cdk9 (T186) antibody P00794 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-12

Answer

The anti-Phospho-Cdk9 (T186) antibody (P00794) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-12

Question

Can you help my question with product P00794, anti-Phospho-Cdk9 (T186) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

E. Banerjee

Verified customer

Asked: 2018-06-11

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00794 anti-Phospho-Cdk9 (T186) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-11

Question

My boss were satisfied with the WB result of your anti-Phospho-Cdk9 (T186) antibody. However we have seen positive staining in liver nucleus. cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is CDK9 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-12-28

Answer

Based on literature, liver does express CDK9. Generally CDK9 expresses in nucleus. cytoplasm. nucleus, pml body. Regarding which tissues have CDK9 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2017-12-28

Question

Would anti-Phospho-Cdk9 (T186) antibody P00794 work for WB with right ovary?

Verified Customer

Verified customer

Asked: 2017-11-20

Answer

According to the expression profile of right ovary, CDK9 is highly expressed in right ovary. So, it is likely that anti-Phospho-Cdk9 (T186) antibody P00794 will work for WB with right ovary.

Boster Scientific Support

Answered: 2017-11-20

Question

you antibody to test anti-Phospho-Cdk9 (T186) antibody P00794 on mouse right ovary for research purposes, then I may be interested in using anti-Phospho-Cdk9 (T186) antibody P00794 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Edwards

Verified customer

Asked: 2017-03-29

Answer

The products we sell, including anti-Phospho-Cdk9 (T186) antibody P00794, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-03-29

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-Phospho-Cdk9 (T186) antibody P00794. Let me know if you need anything else.

A. Carter

Verified customer

Asked: 2014-02-24

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-02-24

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
P00794
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.