Human EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Product Name

Human EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all EGF products

SKU/Catalog Number

EZ0325

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0325)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N971-R1023

Assay Range

4.7 pg/ml - 300 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0325 is reactive to EGF in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EZ0325 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0325-CA	Rabbit anti- human EGF polyclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0325-DA	Biotinylated goat anti- human EGF polyclonal antibody (Detection Antibody)	500 µl, 6 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0325-ST	Lyophilized recombinant human EGF standard	1 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.046	0.092	0.151	0.265	0.521	0.943	1.504	1.965

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Gene/Protein Information For EGF (Source: Uniprot.Org, NCBI)

Alternative Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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