Anti-Phospho-MAPKAPK-2 (Thr334) Antibody

MAPKAPK2 antibody

Boster Bio Anti-Phospho-MAPKAPK-2 (Thr334) Antibody (Catalog # P01193). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P01193
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

Customers Who Bought This Also Bought

Product Name

Anti-Phospho-MAPKAPK-2 (Thr334) Antibody

View all MAPKAPK2 Antibodies

SKU/Catalog Number

P01193

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-MAPKAPK-2 (Thr334) Antibody (Catalog # P01193). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-MAPKAPK-2 (Thr334) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01193)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK-2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01193 is reactive to MAPKAPK2 in Human, Mouse, Rat

Applications

P01193 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

45.568kDa

Background of MAPKAPK2

MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Rouse, J. et al. (1994) Cell 78, 1027-1037.
Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 49kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Gene/Protein Information For MAPKAPK2 (Source: Uniprot.org, NCBI)

Gene Name

MAPKAPK2

Full Name

MAP kinase-activated protein kinase 2

Weight

45.568kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.1; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAPK2; MAPKAPK-2; mitogen-activated protein kinase-activated protein kinase 2; MK2; Rps6kc1 MAPKAPK2 MAPKAP-K2, MK-2, MK2 MAPK activated protein kinase 2 MAP kinase-activated protein kinase 2|MAPKAP kinase 2|mitogen-activated protein kinase-activated protein kinase 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAPKAPK2, check out the MAPKAPK2 Infographic

MAPKAPK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAPKAPK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for P01193

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Phospho-MAPKAPK-2 (Thr334) Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Phospho-MAPKAPK-2 (Thr334) Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-Phospho-MAPKAPK-2 (Thr334) Antibody

Question

I was wanting to use your anti-Phospho-MAPKAPK-2 (Thr334) antibody for IF for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

It shows on the product datasheet, P01193 anti-Phospho-MAPKAPK-2 (Thr334) antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-02

Question

We want to test anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 on rat cervix carcinoma for research purposes, then I may be interested in using anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The products we sell, including anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-31

Question

I see that the anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-20

Question

We are currently using anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

The anti-Phospho-MAPKAPK-2 (Thr334) antibody (P01193) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-05

Question

Does anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 work on goat WB with skin testis?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

Our lab technicians have not validated anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat skin testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-17

Question

Do you have a BSA free version of anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 available?

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-09-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Phospho-MAPKAPK-2 (Thr334) antibody P01193. Let me know if you need anything else.

W. Johnson

Verified customer

Asked: 2016-09-23

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-09-23

Order DetailsPrice
P01193-100uL

100uL

$360

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
P01193
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.