Anti-PP2B-A alpha PPP3CA Monoclonal Antibody

Boster Bio Anti-PP2B-A alpha PPP3CA Monoclonal Antibody catalog # A03026-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A03026-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: WB

Product Name

Anti-PP2B-A alpha PPP3CA Monoclonal Antibody

See all Calcineurin A products

SKU/Catalog Number

A03026-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PP2B-A alpha PPP3CA Monoclonal Antibody catalog # A03026-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PP2B-A alpha PPP3CA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03026-1)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Reactive Species

A03026-1 is reactive to PPP3CA in Human, Mouse, Rat

Applications

A03026-1 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PPP3CA (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08209

Gene ID

5530

Gene Name

PPP3CA

Full Name

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Weight

58.688kDa

Superfamily

PPP phosphatase family

Alternative Names

calcineurin A alpha; Calcineurin A; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNAcatalytic subunit, alpha isoform; CAM-PRP catalytic subunit; CNA; CNA1; CNA1CALNA1; EC 3.1.3.16; PP2B; PP3CA; PPP2B; PPP2BCCN1; PPP3CA; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha); protein phosphatase 3, catalytic subunit, alpha isozyme; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform PPP3CA ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B protein phosphatase 3 catalytic subunit alpha serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform|CAM-PRP catalytic subunit|calcineurin A alpha|protein phosphatase 3 catalytic subunit alpha isozyme

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PPP3CA, check out the PPP3CA Infographic

PPP3CA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PPP3CA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A03026-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Buckwheat Rutin Inhibits AngII-induced Cardiomyocyte Hypertrophy via Blockade of CaN-dependent Signal Pathway

Have you used Anti-PP2B-A alpha PPP3CA Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-PP2B-A alpha PPP3CA Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

14 Customer Q&As for Anti-PP2B-A alpha PPP3CA Monoclonal Antibody

Question

We were happy with the WB result of your anti-PP2B-A alpha Monoclonal antibody . However we have observed positive staining in pancreas cytoplasm using this antibody. Is that expected? Could you tell me where is PPP3CA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

According to literature, pancreas does express PPP3CA. Generally PPP3CA expresses in cytoplasm. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-27

Question

My lab would like using your anti-PP2B-A alpha Monoclonal antibody for skeletal muscle tissue regeneration studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

C. Carter

Verified customer

Asked: 2019-11-08

Answer

We appreciate your inquiry. This A03026-1 anti-PP2B-A alpha Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-08

Question

Can you help my question with product A03026-1, anti-PP2B-A alpha Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03026-1 anti-PP2B-A alpha Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-29

Question

I was wanting to use your anti-PP2B-A alpha Monoclonal antibody for WB for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?

D. Walker

Verified customer

Asked: 2019-09-11

Answer

You can see on the product datasheet, A03026-1 anti-PP2B-A alpha Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-11

Question

I am interested in to test anti-PP2B-A alpha Monoclonal antibody A03026-1 on human pancreas for research purposes, then I may be interested in using anti-PP2B-A alpha Monoclonal antibody A03026-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Jones

Verified customer

Asked: 2019-04-05

Answer

The products we sell, including anti-PP2B-A alpha Monoclonal antibody A03026-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-04-05

Question

I see that the anti-PP2B-A alpha Monoclonal antibody A03026-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-02-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-05

Question

Does anti-PP2B-A alpha Monoclonal antibody A03026-1 work for WB with pancreas?

Verified Customer

Verified customer

Asked: 2018-07-05

Answer

According to the expression profile of pancreas, PPP3CA is highly expressed in pancreas. So, it is likely that anti-PP2B-A alpha Monoclonal antibody A03026-1 will work for WB with pancreas.

Boster Scientific Support

Answered: 2018-07-05

Question

Is there a BSA free version of anti-PP2B-A alpha Monoclonal antibody A03026-1 available?

Verified Customer

Verified customer

Asked: 2017-06-01

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PP2B-A alpha Monoclonal antibody A03026-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-01

Question

Please see the WB image, lot number and protocol we used for pancreas using anti-PP2B-A alpha Monoclonal antibody A03026-1. Please let me know if you require anything else.

S. Collins

Verified customer

Asked: 2016-08-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-08-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-PP2B-A alpha Monoclonal antibody A03026-1. Let me know if you need anything else.

L. Moore

Verified customer

Asked: 2016-02-08

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-02-08

Question

We are currently using anti-PP2B-A alpha Monoclonal antibody A03026-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

W. Parker

Verified customer

Asked: 2014-03-18

Answer

The anti-PP2B-A alpha Monoclonal antibody (A03026-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-03-18

Question

Is a blocking peptide available for product anti-PP2B-A alpha Monoclonal antibody (A03026-1)?

E. Li

Verified customer

Asked: 2013-07-02

Answer

We do provide the blocking peptide for product anti-PP2B-A alpha Monoclonal antibody (A03026-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-07-02

Question

We have been able to see staining in mouse brain. What should we do? Is anti-PP2B-A alpha Monoclonal antibody supposed to stain brain positively?

A. Krishna

Verified customer

Asked: 2013-04-05

Answer

Based on literature brain does express PPP3CA. Based on Uniprot.org, PPP3CA is expressed in caudate nucleus, brain, amygdala, pancreas, erythroleukemia, among other tissues. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-04-05

Question

Does A03026-1 anti-PP2B-A alpha Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Lewis

Verified customer

Asked: 2013-01-24

Answer

You can see on the product datasheet, A03026-1 anti-PP2B-A alpha Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-01-24

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A03026-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.