Click image to see more details
Product Info Summary
| SKU: | M03026 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Calcineurin A Rabbit Monoclonal Antibody
SKU/Catalog Number
M03026
BM5087 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Calcineurin A Rabbit Monoclonal Antibody catalog # M03026. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calcineurin A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03026)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABOG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calcineurin A
Reactive Species
M03026 is reactive to PPP3CA in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
58.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03026 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human MCF-7 whole cell, human U2OS whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of PPP3CA using anti-PPP3CA antibody (M03026).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CA antigen affinity purified monoclonal antibody (M03026) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP3CA at approximately 59 kDa. The expected band size for PPP3CA is at 59 kDa.
Specific Publications For Anti-Calcineurin A Rabbit Monoclonal Antibody (M03026)
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16 Customer Q&As for Anti-Calcineurin A Rabbit Monoclonal Antibody
Question
We are currently using anti-Calcineurin A Rabbit Monoclonal antibody M03026 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The anti-Calcineurin A Rabbit Monoclonal antibody (M03026) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-12
Question
We have been able to see staining in mouse brain. Any tips? Is anti-Calcineurin A Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
From what I have seen in literature brain does express PPP3CA. From what I have seen in Uniprot.org, PPP3CA is expressed in caudate nucleus, brain, amygdala, pancreas, erythroleukemia, among other tissues. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-27
Question
See attached the WB image, lot number and protocol we used for erythroleukemia using anti-Calcineurin A Rabbit Monoclonal antibody M03026. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-19
Question
Can you help my question with product M03026, anti-Calcineurin A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03026 anti-Calcineurin A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-18
Question
I was wanting to use your anti-Calcineurin A Rabbit Monoclonal antibody for ICC for rat erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
You can see on the product datasheet, M03026 anti-Calcineurin A Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-17
Question
We were content with the WB result of your anti-Calcineurin A Rabbit Monoclonal antibody. However we have been able to see positive staining in pancreas cytoplasm using this antibody. Is that expected? Could you tell me where is PPP3CA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
From what I have seen in literature, pancreas does express PPP3CA. Generally PPP3CA expresses in cytoplasm. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-10-03
Question
I am looking for to test anti-Calcineurin A Rabbit Monoclonal antibody M03026 on rat erythroleukemia for research purposes, then I may be interested in using anti-Calcineurin A Rabbit Monoclonal antibody M03026 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The products we sell, including anti-Calcineurin A Rabbit Monoclonal antibody M03026, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Calcineurin A Rabbit Monoclonal antibody M03026. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-26
Question
Would M03026 anti-Calcineurin A Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Bhatt
Verified customer
Asked: 2018-01-26
Answer
You can see on the product datasheet, M03026 anti-Calcineurin A Rabbit Monoclonal antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-26
Question
Is a blocking peptide available for product anti-Calcineurin A Rabbit Monoclonal antibody (M03026)?
L. Lewis
Verified customer
Asked: 2017-07-24
Answer
We do provide the blocking peptide for product anti-Calcineurin A Rabbit Monoclonal antibody (M03026). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-24
Question
We need using your anti-Calcineurin A Rabbit Monoclonal antibody for epileptic encephalopathy studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
Thank you for your inquiry. This M03026 anti-Calcineurin A Rabbit Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for Flow Cytometry, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-07-19
Question
We bought anti-Calcineurin A Rabbit Monoclonal antibody for WB on amygdala in the past. I am using rat, and We want to use the antibody for IF next. My question regards examining amygdala as well as caudate nucleus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
I have checked the website and datasheets of our anti-Calcineurin A Rabbit Monoclonal antibody and I see that M03026 has been validated on rat in both WB and IF. Thus M03026 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-29
Question
Is this M03026 anti-Calcineurin A Rabbit Monoclonal antibody reactive to the isotypes of PPP3CA?
A. Patel
Verified customer
Asked: 2016-03-14
Answer
The immunogen of M03026 anti-Calcineurin A Rabbit Monoclonal antibody is A synthesized peptide derived from human Calcineurin A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-03-14
Question
Does anti-Calcineurin A Rabbit Monoclonal antibody M03026 work for ICC with erythroleukemia?
R. Williams
Verified customer
Asked: 2015-10-07
Answer
According to the expression profile of erythroleukemia, PPP3CA is highly expressed in erythroleukemia. So, it is likely that anti-Calcineurin A Rabbit Monoclonal antibody M03026 will work for ICC with erythroleukemia.
Boster Scientific Support
Answered: 2015-10-07
Question
I see that the anti-Calcineurin A Rabbit Monoclonal antibody M03026 works with ICC, what is the protocol used to produce the result images on the product page?
T. Williams
Verified customer
Asked: 2015-07-20
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-07-20
Question
Do you have a BSA free version of anti-Calcineurin A Rabbit Monoclonal antibody M03026 available?
B. Miller
Verified customer
Asked: 2014-12-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calcineurin A Rabbit Monoclonal antibody M03026 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-12-25
