Anti-Calcineurin A/PPP3CA Antibody Picoband™

Calcineurin A antibody

Boster Bio Anti-Calcineurin A/PPP3CA Antibody Picoband™ catalog # PB9382. Tested in WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PB9382
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Calcineurin A/PPP3CA Antibody Picoband™

View all Calcineurin A Antibodies

SKU/Catalog Number

PB9382

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calcineurin A/PPP3CA Antibody Picoband™ catalog # PB9382. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calcineurin A/PPP3CA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9382)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Calcineurin A, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9382 is reactive to PPP3CA in Human

Applications

PB9382 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

84 kDa

Calculated molecular weight

58.688kDa

Background of Calcineurin A

Calcineurin A is also known as PPP3CA. It is mapped to 4q24. Semsarian et al. (1999) and Musaro et al. (1999) independently showed that IGF1stimulates skeletal muscle hypertrophy and a switch to glycolytic metabolism by activating calcineurin A and inducing the nuclear translocation of transcription factor NFATC1. Semsarian et al. (1999) found that hypertrophy was suppressed by the calcineurin inhibitors cyclosporin A or FK506, but not by inhibitors of the MAP kinase or phosphatidylinositol-3-OH kinase pathways. Musaro et al. (1999) showed that expression of a dominant-negative calcineurin mutant also repressed myocyte differentiation and hypertrophy. Musaro et al. (1999) demonstrated that either IGF1 or activated calcineurin induces expression of transcription factor GATA2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of NFATC1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For PPP3CA (Source: Uniprot.org, NCBI)

Gene Name

PPP3CA

Full Name

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Weight

58.688kDa

Superfamily

PPP phosphatase family

Alternative Names

calcineurin A alpha; Calcineurin A; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNAcatalytic subunit, alpha isoform; CAM-PRP catalytic subunit; CNA; CNA1; CNA1CALNA1; EC 3.1.3.16; PP2B; PP3CA; PPP2B; PPP2BCCN1; PPP3CA; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha); protein phosphatase 3, catalytic subunit, alpha isozyme; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform PPP3CA ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B protein phosphatase 3 catalytic subunit alpha serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform|CAM-PRP catalytic subunit|calcineurin A alpha|protein phosphatase 3 catalytic subunit alpha isozyme

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPP3CA, check out the PPP3CA Infographic

PPP3CA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPP3CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9382 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Buckwheat Rutin Inhibits AngII-induced Cardiomyocyte Hypertrophy via Blockade of CaN-dependent Signal Pathway

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4 Customer Q&As for Anti-Calcineurin A/PPP3CA Antibody Picoband™

Question

We are currently using anti-Calcineurin A/PPP3CA antibody PB9382 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

The anti-Calcineurin A/PPP3CA antibody (PB9382) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-31

Question

We have observed staining in human amygdala. Are there any suggestions? Is anti-Calcineurin A/PPP3CA antibody supposed to stain amygdala positively?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

From what I have seen in literature amygdala does express PPP3CA. From what I have seen in Uniprot.org, PPP3CA is expressed in caudate nucleus, brain, amygdala, pancreas, erythroleukemia, among other tissues. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-27

Question

We were content with the WB result of your anti-Calcineurin A/PPP3CA antibody. However we have observed positive staining in amygdala cytoplasm using this antibody. Is that expected? Could you tell me where is PPP3CA supposed to be expressed?

W. Baker

Verified customer

Asked: 2017-01-13

Answer

From what I have seen in literature, amygdala does express PPP3CA. Generally PPP3CA expresses in cytoplasm. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-01-13

Question

you antibody using your anti-Calcineurin A/PPP3CA antibody for calcineurin-mediated signaling studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

A. Banerjee

Verified customer

Asked: 2016-10-17

Answer

Thank you for your inquiry. This PB9382 anti-Calcineurin A/PPP3CA antibody is tested on hela whole cell lysate, a549 whole cell lysate, colo320 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-10-17

Order DetailsPrice
PB9382

100μg

$370
PB9382-10ug

10μg sample (liquid)

$99
PB9382-Biotin

100 μg Biotin conjugated

$570
PB9382-Cy3

100 μg Cy3 conjugated

$570
PB9382-Dylight488

100 μg Dylight488 conjugated

$570
PB9382-Dylight550

100 μg Dylight550 conjugated

$570
PB9382-Dylight594

100 μg Dylight594 conjugated

$570
PB9382-FITC

100 μg FITC conjugated

$570
PB9382-HRP

100 μg HRP conjugated

$570
PB9382-APC

100 μg APC conjugated

$670
PB9382-PE

100 μg PE conjugated

$670
PB9382-carrier-free

Carrier Free

$370

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