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- Table of Contents
TRAP1 antibody (PB9834) performs well in IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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IHC analysis of TRAP1 using anti-TRAP1 antibody (PB9834).
TRAP1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRAP1 Antibody (PB9834) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
TRAP1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRAP1 Antibody (PB9834) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-TRAP1 Antibody Picoband®
TRAP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IHC, WB |
Tested Applications
TRAP1 antibody was positively detected by WB in:
Rat Cardiac Muscle Tissue, Rat Kidney Tissue, Rat Brain Tissue, SMMC Whole Cell, PANC Whole Cell, A549 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TRAP1 antibody was positively detected by IHC in:
human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Human
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TRAP1 Antibody Picoband®
View all TRAP1 Antibodies
View all TRAP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRAP1 recombinant protein (Position: A571-H704). Human TRAP1 shares 91.7% and 94% amino acid (aa) sequence identity with mouse and rat TRAP1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated Molecular Weight
80.1 kDa
Background of TRAP1
Heat shock protein 75 kDa, mitochondrial is a protein that in humans is encoded by the TRAP1 gene. It is mapped to 16p13.3. This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. And this protein may function in regulating cellular stress responses. In addition, it was found that TRAP1 interacted with the N-terminal half of TNFR1. Also, TRAP1 interacted with the C-terminal ends of the proteins encoded by both multiple exostoses-causing genes, EXT1 and EXT2, but not with EXTL1 or EXTL3.
Cite This Product
Anti-TRAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9834)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rat
93%
High Homology
Mouse
91%
High Homology
Cow
88%
Moderate Homology
Sheep
88%
Moderate Homology
Dog
87%
Moderate Homology
Zebrafish
70%
Moderate Homology
Drosophila
49%
Low homology
Rabbit
1%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TRAP1 antibody
Immunohistochemistry (IHC) protocol for TRAP1 antibody
Publications
Application:
Species:
Melatonin combined with static magnetic field acquire better protective effects on bone mass and bone strength in osteoporosis induced by ovariectomy in rats
Published: 2025-06-02
Journal: ANNALS OF MEDICINE
Application: IHC
Impact Factor: 4.30
Species: Rat
PMID: 40454678
Proteomic analysis of liver mitochondria of db/db mice treated with grape seed procyanidin B2
Published: 2020-08-19
Journal: JOURNAL OF FOOD BIOCHEMISTRY
Application: WB
Impact Factor: 1.66
Species: Mouse
PMID: 32815169
Expression of TRAF6 Protein and TRAF6 mRNA during Different Stages of Deciduous Tooth Root Physiological Absorption
Published: 2013-02-27
Journal: Journal of Hard Tissue Biology
Application: IHC
Impact Factor: 0.17
Species: Human
Protective effects of Polygonatum sibiricum polysaccharide on ovariectomy-induced bone loss in rats
Published: 2011-04-28
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 2.47
Species: Rat
PMID: 21550389
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Product Promise
We promise Anti-TRAP1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB9834
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026