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Product Info Summary
| SKU: | A00379 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Survivin/BIRC5 Antibody Picoband®
SKU/Catalog Number
A00379
Size
100 μg/vial
Form
Lyophilized
Description
Bio Anti-Survivin/BIRC5 Antibody Picoband® catalog # A00379. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.Target: Survivin/BIRC5 (IAP family); function/pathways: inhibitor of caspase activation → negative regulation of apoptosis; expression context: highly expressed in most human tumors and fetal tissue; assay contexts supported here: IHC (paraffin), WB, ELISA; use-case anchor: apoptosis signaling and tumor biology. For immune-panel context compare lymphoid marker CD19; in tumor studies, may be profiled alongside ECM remodeling marker MMP1 for complementary pathway coverage.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Survivin/BIRC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00379)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Survivin recombinant protein (Position: M1-D142). Human Survivin shares 84.3% and 83% amino acid (aa) sequence identity with mouse and rat Survivin, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00379 is reactive to BIRC5 in Human, Mouse, Rat
Observed Molecular Weight
16 kDa
Calculated molecular weight
16.4 kDa
Background of BIRC5
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. It is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. And this protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00379 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human 293T whole cell
IHC: human lymphoma tissue, mouse thymus tissue
Validation Images & Assay Conditions
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Western blot analysis of Survivin using anti-Survivin antibody (A00379).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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IHC analysis of Survivin using anti-Survivin antibody (A00379).
Survivin was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin Antibody (A00379) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Survivin using anti-Survivin antibody (A00379).
Survivin was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin Antibody (A00379) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Survivin/BIRC5 Antibody Picoband® (A00379)
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Customer Q&As
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16 Customer Q&As for Anti-Survivin/BIRC5 Antibody Picoband®
Question
Our lab were well pleased with the WB result of your anti-Survivin/BIRC5 antibody. However we have seen positive staining in neuroblastoma cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?
E. Krishna
Verified customer
Asked: 2020-04-22
Answer
According to literature, neuroblastoma does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2020-04-22
Question
We have been able to see staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-Survivin/BIRC5 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
From literature cervix carcinoma does express BIRC5. From Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2020-03-16
Question
Here is the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin/BIRC5 antibody A00379. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-02
Question
I would like to test anti-Survivin/BIRC5 antibody A00379 on mouse myeloid leukemia cell for research purposes, then I may be interested in using anti-Survivin/BIRC5 antibody A00379 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The products we sell, including anti-Survivin/BIRC5 antibody A00379, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-21
Question
Do you have a BSA free version of anti-Survivin/BIRC5 antibody A00379 available?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Survivin/BIRC5 antibody A00379 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-Survivin/BIRC5 antibody A00379 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-Survivin/BIRC5 antibody (A00379) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
My lab would like using your anti-Survivin/BIRC5 antibody for separation of sister chromatids studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thanks for your inquiry. This A00379 anti-Survivin/BIRC5 antibody is tested on 293t whole cell lysates, human k562, tissue lysate, k562 whole cell lysate, mouse thymus tissue. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-02
Question
I see that the anti-Survivin/BIRC5 antibody A00379 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-29
Question
Is this A00379 anti-Survivin/BIRC5 antibody reactive to the isotypes of BIRC5?
K. Krishna
Verified customer
Asked: 2018-12-28
Answer
The immunogen of A00379 anti-Survivin/BIRC5 antibody is E. coli-derived human Survivin recombinant protein (Position: M1-D142). Human Survivin shares 84.3% and 83% amino acid (aa) sequence identity with mouse and rat Survivin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-28
Question
Can you help my question with product A00379, anti-Survivin/BIRC5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00379 anti-Survivin/BIRC5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-27
Question
I was wanting to use your anti-Survivin/BIRC5 antibody for WB for mouse myeloid leukemia cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse myeloid leukemia cell identification?
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
It shows on the product datasheet, A00379 anti-Survivin/BIRC5 antibody has been validated for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse myeloid leukemia cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-20
Question
Does A00379 anti-Survivin/BIRC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
As indicated on the product datasheet, A00379 anti-Survivin/BIRC5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-17
Question
Is a blocking peptide available for product anti-Survivin/BIRC5 antibody (A00379)?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
We do provide the blocking peptide for product anti-Survivin/BIRC5 antibody (A00379). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-29
Question
We purchased anti-Survivin/BIRC5 antibody for ELISA on lung in the past. I am using human, and We want to use the antibody for WB next. We want examining lung as well as mammary gland muscle in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
I looked at the website and datasheets of our anti-Survivin/BIRC5 antibody and it seems that A00379 has been validated on human in both ELISA and WB. Thus A00379 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin/BIRC5 antibody A00379. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-19
Question
Will anti-Survivin/BIRC5 antibody A00379 work for WB with myeloid leukemia cell?
R. Zhao
Verified customer
Asked: 2014-11-21
Answer
According to the expression profile of myeloid leukemia cell, BIRC5 is highly expressed in myeloid leukemia cell. So, it is likely that anti-Survivin/BIRC5 antibody A00379 will work for WB with myeloid leukemia cell.
Boster Scientific Support
Answered: 2014-11-21
