Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™

Product Name

Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™

View all Kallikrein 3/PSA Antibodies

SKU/Catalog Number

A01505-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™ catalog # A01505-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01505-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Prostate Specific Antigen/KLK3 recombinant protein (Position: A64-D255).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01505-3 is reactive to KLK3 in Human

Applications

A01505-3 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

28.741kDa

Background of Kallikrein 3/PSA

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. KLK3 is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. This gene is mapped to 19q13.33. KLK3 is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. KLK3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Prostate Specific Antigen/KLK3 using anti-Prostate Specific Antigen/KLK3 antibody (A01505-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human LNCAP whole cell lysates,
Lane 2: human LNCAP whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prostate Specific Antigen/KLK3 antigen affinity purified polyclonal antibody (Catalog # A01505-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prostate Specific Antigen/KLK3 at approximately 34KD. The expected band size for Prostate Specific Antigen/KLK3 is at 34KD.

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Figure 2. IHC analysis of Prostate Specific Antigen/KLK3 using anti-Prostate Specific Antigen/KLK3 antibody (A01505-3).
Prostate Specific Antigen/KLK3 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Prostate Specific Antigen/KLK3 Antibody (A01505-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For KLK3 (Source: Uniprot.org, NCBI)

Alternative Names

APS seminin; EC 3.4.21; EC 3.4.21.77; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin KLK3 APS, KLK2A1, PSA, hK3 kallikrein related peptidase 3 prostate-specific antigen|P-30 antigen|gamma-seminoprotein|kallikrein-3|semenogelase|seminin

For more info on KLK3, check out the KLK3 Infographic

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In this infographic, you will see the following information for KLK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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