Human KLK1/Kallikrein 1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK1. 96wells/kit, with removable strips.
Human KLK1/Kallikrein 1 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human KLK1/Kallikrein 1 ELISA Kit PicoKine™
See all Kallikrein 1 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Kallikrein 1/KLK1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma(heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Kallikrein 1/KLK1 tissue specificity: Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. .|
|Cite This Product||Human KLK1/Kallikrein 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0903)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: I25-S262|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For KLK1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3816|
|Species Of This Entry||Human|
|Superfamily||peptidase S1 family|
|Alternative Names||Kallikrein 1|EC 3.4.21; EC 220.127.116.11; glandular kallikrein 1; hK1; Kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein serine protease 1; kallikrein-1; Kidney/pancreas/salivary gland kallikrein; KLK1; Klk6; KLKR; Tissue kallikrein|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Methylation; Demethylation; Reduction|
|Gene Location||19q13.33, on Chromasome 19, gene sequence: NC_000019.10 (50819146..50823787, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For KLK1 (Source: Uniprot.org, NCBI)
|Protein Function||Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.|
|Tissue Specificity||Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.|
|Related Diseases||Malignant Neoplasms; Neoplasms; Hypertensive Disease; Carcinoma; Prostatic Neoplasms; Malignant Neoplasm Of Prostate; Endometrial Polyp; Tumor Angiogenesis; Malignant Neoplasm Of Ovary; Lupus Erythematosus, Systemic; Kidney Diseases; Kidney Neoplasm; Nephritis; Malignant Neoplasm Of Breast; Lupus Nephritis; Fibrosis; Renal Cell Carcinoma; Ischemia; Carcinogenesis; Essential Hypertension|
|Related Pathways||Angiogenesis; Localization; Excretion; Secretion; Transport; Cell Adhesion; Wound Healing; Vasodilation; Methylation; Keratinocyte Migration; Tissue Remodeling; Pathogenesis; Cell Migration; Seminal Clot Liquefaction; Proteolysis; Immune Response; Muscle Contraction; Diuresis; Embryo Implantation; Innate Immune Response|
|Background||Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.|
|Scientific References||PMID: 3004571 by Fukushima D., et al. Nucleotide sequence of cloned cDNA for human pancreatic kallikrein.|
PMID: 2898948 by Evans B.A., et al. Structure and chromosomal localization of the human renal kallikrein gene.
Kit Components And Assay QC Data Details Of Human KLK1/Kallikrein 1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0903-CAP||96-well plate precoated with anti-Human Kallikrein 1 antibody||1|
|EK0903-ST||lyophilized recombinant Human Kallikrein 1 standard||10ng/tube|
|EK0903-DA||biotinylated anti-Human Kallikrein 1 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0903 Human KLK1/Kallikrein 1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human KLK1/Kallikrein 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human KLK1/Kallikrein 1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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