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KLK3 antibody (PB9259) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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IHC analysis of KLK3 using anti-KLK3 antibody (PB9259).
KLK3 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLK3 Antibody (PB9259) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
KLK3 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLK3 Antibody (PB9259) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-Prostate Specific Antigen/KLK3 Antibody Picoband®
KLK3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
KLK3 antibody was positively detected by WB in:
human LNCAP whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KLK3 antibody was positively detected by IHC in:
human prostatic cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
1-2 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Prostate Specific Antigen/KLK3 Antibody Picoband®
View all KLK3 Antibodies
View all KLK3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PSA recombinant protein (Position: I25-P261). Human PSA shares 54% amino acid (aa) sequence identity with rat PSA.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated Molecular Weight
28.7 kDa
Background of KLK3
Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. KLK3 is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. This gene is mapped to 19q13.33. KLK3 is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. KLK3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia.
Cite This Product
Anti-Prostate Specific Antigen/KLK3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9259)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
89%
Moderate Homology
Cow
61%
Low homology
Mouse
57%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KLK3 antibody
Immunohistochemistry (IHC) protocol for KLK3 antibody
Publications
Application:
Species:
Hot melt extrusion-enhanced Angelica gigas alleviates benign prostatic hyperplasia via AR/ERK signaling: A network pharmacology-based mechanistic study
Published: 2025-11-08
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 8.30
Species: Human,Rat
PMID: 41270388
Antioxidant mitoquinone suppresses benign prostatic hyperplasia by regulating the AR–NLRP3 pathway
Published: 2023-07-11
Journal: Redox Biology
Application: WB
Impact Factor: 11.40
Species: Human,Rat
PMID: 37454529
Hesperidin ameliorates benign prostatic hyperplasia by attenuating cell proliferation, inflammatory response, and epithelial-mesenchymal transition via the TGF-β1/Smad signaling pathway
Published: 2023-02-13
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 7.50
Species: Mouse,Human
PMID: 36791565
A Combination of Natural Products, BenPros (Green Tea Extract, Soybean Extract and Camellia Japonica Oil), Ameliorates Benign Prostatic Hyperplasia
Published: 2022-06-16
Journal: Applied Sciences-Basel
Application: WB
Impact Factor: 2.84
Species: Human
Umbelliferone Ameliorates Benign Prostatic Hyperplasia by Inhibiting Cell Proliferation and G1/S Phase Cell Cycle Progression through Regulation of STAT3/E2F1 Axis
Published: 2021-08-21
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 5.92
Species: Human
PMID: 34445725
Psoralea corylifolia L. extract ameliorates benign prostatic hyperplasia by regulating prostate cell proliferation and apoptosis
Published: 2021-01-21
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.36
Species: Human
PMID: 33485982
Anti-Proliferative Effects of Standardized Cornus officinalis on Benign Prostatic Epithelial Cells via the PCNA/E2F1-Dependent Cell Cycle Pathway
Published: 2020-12-15
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.56
Species: Human
PMID: 33334082
Baicalin alleviates benign prostate hyperplasia through androgen-dependent apoptosis
Published: 2020-02-04
Journal: Aging-US
Application: WB
Impact Factor: 4.83
Species: Rat
PMID: 32018227
6′-Sialyllactose Ameliorates In Vivo and In Vitro Benign Prostatic Hyperplasia by Regulating the E2F1/pRb–AR Pathway
Published: 2019-09-12
Journal: Nutrients
Application: WB
Impact Factor: 4.17
Species: Rat
PMID: 31547405
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Product Promise
We promise Anti-Prostate Specific Antigen/KLK3 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9259
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026