Human Kallikrein 3 PicoKine™ ELISA Kit
|Size||96wells/kit, with removable strips.|
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Product Name||Human Kallikrein 3 PicoKine™ ELISA Kit|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Size||96wells/kit, with removable strips.|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallikrein 3. 96wells/kit, with removable strips.|
|Cite This Product||Human Kallikrein 3 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0816)|
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: A18-P261|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Antibody Clonalities||Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat
|EK0816-CAP||96-well plate precoated with anti-Human KLK3 antibody||1|
|EK0816-ST||lyophilized recombinant Human KLK3 standard||20ng/tube|
|EK0816-DA||biotinylated anti-Human KLK3 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Human Kallikrein 3 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Prostate-specific antigen|
|Alternative Names||Prostate-specific antigen;PSA;220.127.116.11;Gamma-seminoprotein;Seminin;Kallikrein-3;P-30 antigen;Semenogelase;KLK3;APS;|
|Molecular Weight||28741 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.|
|Research Areas||Cell Type Markers, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Neuroscience, Signal Transduction, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens
*You can search these to find other products in these research areas.
|Background||Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (EK0816)
Promotion:Order 4+ of the same ELISA kit and get 20% off| Order 20+ of the same ELISA kit and get 30% off .
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
Keywords: component, ingredient
Keywords: expiration, storage, temperature, size