Human Kallikrein 3 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallikrein 3. 96wells/kit, with removable strips.
Human Kallikrein 3 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human Kallikrein 3 ELISA Kit PicoKine™
See all Kallikrein 3/PSA primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Psa/KLK3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Psa/KLK3 tissue specificity:|
|Cite This Product||Human Kallikrein 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0816)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: A18-P261|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For KLK3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||354|
|Species Of This Entry||Human|
|Protein Name||Prostate-specific antigen|
|Superfamily||peptidase S1 family|
|Alternative Names||Kallikrein 3/PSA|APS seminin; EC 3.4.21; EC 188.8.131.52; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin|
|Post Tranlational Modifications||Cleavage; Methylation; Phosphorylation; Demethylation; Acetylation|
|Gene Location||19q13.33, on Chromasome 19, gene sequence: NC_000019.10 (50854915..50860764)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For KLK3 (Source: Uniprot.org, NCBI)
|Protein Function||Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.|
|Research Areas||Cancer; Cellular Markers; Prostate Cancer; Tumor Biomarkers|
|Related Diseases||Malignant Neoplasms; Malignant Neoplasm Of Prostate; Prostatic Neoplasms; Neoplasms; Carcinoma; Malignant Neoplasm Of Breast; Malignant Paraganglionic Neoplasm; Neoplasm Metastasis; Tumor Angiogenesis; Adenocarcinoma; Mammary Neoplasms; Carcinogenesis; Benign Prostatic Hypertrophy; Prostatic Hyperplasia; Hyperplasia; Cell Transformation, Neoplastic; Ovarian Neoplasm; Malignant Neoplasm Of Ovary; Hypertensive Disease|
|Related Pathways||Angiogenesis; Tube Formation; Pathogenesis; Cell Proliferation; Localization; Methylation; Dna Methylation; Rna Splicing; Cell Growth; Secretion; Transport; Seminal Clot Liquefaction; Immune Response; Proteolysis; Reflex; Reverse Transcription; Embryo Implantation; Innate Immune Response; Mating; Regulation Of Blood Pressure|
|Background||Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3|
|Scientific References||PMID: 2436946 by Lundwall A., et al. Molecular cloning of human prostate specific antigen cDNA.|
PMID: 2467258 by Digby M.R., et al. Human prostate specific antigen (PSA) gene: structure and linkage to the kallikrein-like gene, hGK-1.
Kit Components And Assay QC Data Details Of Human Kallikrein 3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0816-CAP||96-well plate precoated with anti-Human Kallikrein 3/PSA antibody||1|
|EK0816-ST||lyophilized recombinant Human Kallikrein 3/PSA standard||20ng/tube|
|EK0816-DA||biotinylated anti-Human Kallikrein 3/PSA antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0816 Human Kallikrein 3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human Kallikrein 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human Kallikrein 3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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