Anti-Protein orai-2 ORAI2 Antibody

Product Name

Anti-Protein orai-2 ORAI2 Antibody

View all ORAI2 Antibodies

SKU/Catalog Number

A07411

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein orai-2 ORAI2 Antibody (Catalog # A07411). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

ORAI2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protein orai-2 ORAI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07411)

Host

Rabbit

Contents

ORAI2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ORAI2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2. The immunogen is located within the last 50 amino acids of ORAI2.

Cross-reactivity

At least two isoforms of ORAI2 are known to exist; this antibody will detect both isoforms. ORAI antibody is predicted not to cross-react with ORAI1 or ORAI3.

Reactive Species

A07411 is reactive to ORAI2 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

28.6 kDa

Background of ORAI2

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07411 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ORAI2

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