|Product Name||Anti-RAGE/AGER Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Advanced glycosylation end product-specific receptor(AGER) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-RAGE/AGER Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1069)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human RAGE(174-192aa KEQTRRHPETGLFTLQSEL), different from the related mouse and rat sequences by two amino acids.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Anti-RAGE antibody, PA1069, Western blotting
Lane 1: Recombinant Human RAGE Protein 10ng
Lane 2: Recombinant Human RAGE Protein 5ng
Lane 3: Recombinant Human RAGE Protein 2.5ng
Anti-RAGE antibody, PA1069, IHC(P)
IHC(P): Rat Lung Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Advanced glycosylation end product-specific receptor|
|Tissue Specificity||Endothelial cells.|
|Alternative Names||Advanced glycosylation end product-specific receptor;Receptor for advanced glycosylation end products;AGER;RAGE;|
|Subcellular Localization||Isoform 1: Cell membrane; Single-pass type I membrane protein.|
|Molecular Weight||42803 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. .|
|Research Areas||Alzheimer'S Disease, Atherosclerosis, Cardiovascular, Diabetes Associated, Neurodegenerative Disease, Neurology Process, Neuroscience, Sensory System, Vascular Inflammation, Visual System
*You can search these to find other products in these research areas.
|Background||The receptor for advanced glycation end products(RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,