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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IHC, IHC-F, WB|
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Boster Bio Anti-RAGE/AGER Antibody catalog # PA1069. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAGE/AGER Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1069)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence in the middle region of human RAGE (174-192aa KEQTRRHPETGLFTLQSEL), different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1069 is reactive to AGER in Human, Mouse, Rat
PA1069 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Background of AGER
The receptor for advanced glycation end products (RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RAGE using anti-RAGE antibody (PA1069).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE antigen affinity purified polyclonal antibody (Catalog # PA1069) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE at approximately 43 kDa. The expected band size for RAGE is at 43 kDa.
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Anti-RAGE antibody, PA1069, IHC(P)
IHC(P): Rat Lung Tissue
Gene/Protein Information For AGER (Source: Uniprot.org, NCBI)
Advanced glycosylation end product-specific receptor
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1 AGER RAGE, SCARJ1, sRAGE advanced glycosylation end-product specific receptor advanced glycosylation end product-specific receptor|receptor for advanced glycation end-products variant 20*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on AGER, check out the AGER Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGER: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
PA1069 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-?B pathway
Li JY, Lu YH, Zhang LW, Zhou PM, Chen T. Ann Dermatol. 2017 Feb;29(1):121-123. doi: 10.5021/ad.2017.29.1.121. Epub 2017 Feb 3. Increased Serum High Mobility Group Box 1 (HMGB1) Concentration and the Altered Expression of HMGB1 and Its Receptor Adv...
Wang F, He Q, Wang J, Yuan Q, Guo H, Chai L, Wang S, Hu L, Zhang Y. BMC Complement Altern Med. 2017 May 10;17(1):258. doi: 10.1186/s12906-017-1738-8. Neuroprotective effect of salvianolate lyophilized injection against cerebral ischemia in type 1 ...
Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Cardiovasc Diabetol. 2013 Feb 26;12:37. Doi: 10.1186/1475-2840-12-37. Heparanase Induced By Advanced Glycation End Products (Ages) Promotes Macrophage Migration Involving Rage And Pi3K/Akt Pathway.
Liu J, Wang S, Feng L, Ma D, Fu Q, Song Y, Jia X, Ma S. J Med Food. 2013 Jul;16(7):577-86. Doi: 10.1089/Jmf.2012.2654. Hypoglycemic And Antioxidant Activities Of Paeonol And Its Beneficial Effect On Diabetic Encephalopathy In Streptozotocin-Induce...
Wang L, Zhang X, Liu L, Yang R, Cui L, Li M. Neurosci Lett. 2010 Mar 8;471(3):152-6. Doi: 10.1016/J.Neulet.2010.01.030. Epub 2010 Jan 25. Atorvastatin Protects Rat Brains Against Permanent Focal Ischemia And Downregulates Hmgb1, Hmgb1 Receptors (R...
Wang L, Zhang X, Liu L, Cui L, Yang R, Li M, Du W. Brain Res. 2010 Mar 19;1321:143-51. Doi: 10.1016/J.Brainres.2009.12.046. Epub 2010 Jan 4. Tanshinone Ii A Down-Regulates Hmgb1, Rage, Tlr4, Nf-Kappab Expression, Ameliorates Bbb Permeability And E...
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