Rabbit IgG polyclonal antibody for Advanced glycosylation end product-specific receptor(AGER) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat. Cited in 7 publication(s).
Anti-RAGE/AGER Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-RAGE/AGER Antibody
See all AGER primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for RAGE/AGER detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. RAGE/AGER information: Molecular Weight: 42803 MW; Subcellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Endothelial cells.|
|Cite This Product||Anti-RAGE/AGER Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1069)|
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|Anti-RAGE/AGER Antibody may replace the following items: sc 33662|sc 365154|sc 5563|sc 74473|sc 74535|sc 80652|sc 80653|sc 8229|sc 8230.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human RAGE(174-192aa KEQTRRHPETGLFTLQSEL), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For AGER (Source: Uniprot.org, NCBI)
|NCBI Gene Id||177|
|Species Of This Entry||Human|
|Protein Name||Advanced glycosylation end product-specific receptor|
|Alternative Names||AGER|advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1|
|Post Tranlational Modifications||Glycosylation; Phosphorylation; Biotinylation; Acetylation; Methylation|
|Subcellular Localization||[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 10]: Cell membrane; Single-pass type I membrane protein.|
|Gene Location||6p21.32, on Chromasome 6, gene sequence: NC_000006.12 (32180968..32184380, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For AGER (Source: Uniprot.org, NCBI)
|Protein Function||Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.|
|Tissue Specificity||Endothelial cells.|
|Interacting Proteins||DIAPH1; EGFR; Egfr; GRB2; HMGB1; MAPKAPK5; NCK1; PRKCZ; RHOA; S100B; S100P; TIRAP; TTR|
|Related Diseases||Diabetes Mellitus; Diabetic Nephropathy; Diabetes Mellitus, Non-insulin-dependent; Kidney Diseases; Inflammation; Malignant Neoplasms; Atherosclerosis; Lung Diseases; Fibrosis; Chronic Obstructive Airway Disease; Lung Diseases, Obstructive; Dysequilibrium Syndrome; Malignant Neoplasm Of Pancreas; Infective Disorder; Hypertensive Disease; Vascular Diseases; Alzheimer's Disease; Coronary Artery Disease; Pancreatic Neoplasm; Autoimmune Reaction|
|Related Pathways||Pathogenesis; Glycosylation; Localization; Secretion; Transport; Cell Proliferation; Cell Killing; Insulin Secretion; Immune Response; Autolysis; Methylation; Neuroprotection; Gene Conversion; Cellular Localization; Metabolic Process; Tear Secretion; Autophagy|
|Background||The receptor for advanced glycation end products(RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment.|
|Scientific References||PMID: 1378843 by Neeper M., et al. Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins.|
PMID: 7835890 by Sugaya K., et al. Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3.
Our Boster Quality Guarantee for Anti-RAGE/AGER Antibody covers its use in the following applications.Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human RAGE Protein 10ng
Lane 2: Recombinant Human RAGE Protein 5ng
Lane 3: Recombinant Human RAGE Protein 2.5ng
IHC(P): Rat Lung Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 7
|Wang L, Zhang X, Liu L, Cui L, Yang R, Li M, Du W. Brain Res. 2010 Mar 19;1321:143-51. Doi: 10.1016/J.Brainres.2009.12.046. Epub 2010 Jan 4. Tanshinone Ii A Down-Regulates Hmgb1, Rage, Tlr4, Nf-Kappab Expression, Ameliorates Bbb Permeability And E...||PubMed ID 20043889|
|Wang L, Zhang X, Liu L, Yang R, Cui L, Li M. Neurosci Lett. 2010 Mar 8;471(3):152-6. Doi: 10.1016/J.Neulet.2010.01.030. Epub 2010 Jan 25. Atorvastatin Protects Rat Brains Against Permanent Focal Ischemia And Downregulates Hmgb1, Hmgb1 Receptors (R...||PubMed ID 20100543|
|Liu J, Wang S, Feng L, Ma D, Fu Q, Song Y, Jia X, Ma S. J Med Food. 2013 Jul;16(7):577-86. Doi: 10.1089/Jmf.2012.2654. Hypoglycemic And Antioxidant Activities Of Paeonol And Its Beneficial Effect On Diabetic Encephalopathy In Streptozotocin-Induce...||PubMed ID 23875897|
|Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Cardiovasc Diabetol. 2013 Feb 26;12:37. Doi: 10.1186/1475-2840-12-37. Heparanase Induced By Advanced Glycation End Products (Ages) Promotes Macrophage Migration Involving Rage And Pi3K/Akt Pathway.||PubMed ID 23442498|
|Wang F, He Q, Wang J, Yuan Q, Guo H, Chai L, Wang S, Hu L, Zhang Y. BMC Complement Altern Med. 2017 May 10;17(1):258. doi: 10.1186/s12906-017-1738-8. Neuroprotective effect of salvianolate lyophilized injection against cerebral ischemia in type 1 ...||PubMed ID 28486941|
|Li JY, Lu YH, Zhang LW, Zhou PM, Chen T. Ann Dermatol. 2017 Feb;29(1):121-123. doi: 10.5021/ad.2017.29.1.121. Epub 2017 Feb 3. Increased Serum High Mobility Group Box 1 (HMGB1) Concentration and the Altered Expression of HMGB1 and Its Receptor Adv...||PubMed ID 28223765|
|Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-?B pathway||PubMed ID 27026909|
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Note: sample size takes longer to process, average 5-7 days.
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