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Product Info Summary
| SKU: | A01058T359S363 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-Rsk-1 (phospho-T359/S363) RPS6KA1 Antibody
SKU/Catalog Number
A01058T359S363
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rsk-1 (phospho-T359/S363) RPS6KA1 Antibody catalog # A01058T359S363. Tested in WB,IP,IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rsk-1 (phospho-T359/S363) RPS6KA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01058T359S363)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human Rsk-1 around the phosphorylation site of Thr359/Ser363.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01058T359S363 is reactive to RPS6KA1 in Human, Mouse, Rat
Calculated molecular weight
82.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01058T359S363 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of p-Rsk-1 (T359/S363) pAb at 1:2000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug)
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Immunofluorescence analysis of CT26 cells using Rsk-1 (phospho-T359/S363) antibody at dilution of 1:50.
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Immunoprecipitation of HepG2 cell lysate using p-Rsk-1 (T359/S363) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using p-Rsk-1 (T359/S363) #BS4276. “↑”（supernatant）; “↓(deposition）
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Immunohistochemistry of paraffin-embedded Rat Brain using Rsk-1 (phospho-T359/S363) antibody at dilution of 1:50.
Specific Publications For Anti-Rsk-1 (phospho-T359/S363) RPS6KA1 Antibody (A01058T359S363)
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