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- Table of Contents
PLG antibody (A03674-1) performs well in Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of U251 cells using anti-Plasminogen antibody (A03674-1).
Overlay histogram showing U251 cells stained with A03674-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasminogen Antibody (A03674-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U251 cells stained with A03674-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasminogen Antibody (A03674-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Plasminogen/PLG Antibody Picoband®
PLG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, WB |
Tested Applications
PLG antibody was positively detected by WB in:
human HL-60 whole cell, human HEK293 whole cell, human Caco-2 whole cell, human Sw620 whole cell, human Jurkat whole cell, human K562 whole cell, rat liver tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PLG antibody was positively detected by FC in:
U251 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Monkey
Predicted
Product Information
Product Name
Anti-Plasminogen/PLG Antibody Picoband®
View all PLG Antibodies
View all PLG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Plasminogen, which shares 72.7% amino acid (aa) sequence identity with both mouse and rat Plasminogen.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
90.6 kDa
Background of PLG
Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU) and tissue plasminogen activator (PLAT). The main function of plasmin is to dissolve fibrin (see, e.g., FGA) clots. Plasmin, like trypsin, belongs to the family of serine proteinases. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis.
Cite This Product
Anti-Plasminogen/PLG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03674-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
95%
High Homology
Monkey
91%
High Homology
Dog
82%
Moderate Homology
Mouse
73%
Moderate Homology
Rat
73%
Moderate Homology
Cow
68%
Low homology
Sheep
64%
Low homology
Zebrafish
32%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PLG antibody
Flow Cytometry (FC) protocol for PLG antibody
Publications
Application:
Species:
Mycoplasma synoviae LP78 is a fibronectin/plasminogen binding protein, putative adhesion, and potential diagnostic antigen
Published: 2024-01-09
Journal: Frontiers in Microbiology
Application: WB
Impact Factor: 4.00
Species: Chicken
PMID: 38264482
A multifunctional enolase mediates cytoadhesion and interaction with host plasminogen and fibronectin in Mycoplasma hyorhinis
Published: 2022-03-25
Journal: VETERINARY RESEARCH
Application: WB
Impact Factor: 3.83
Species: M. hyorhinis
PMID: 35337383
Increased plasmin-mediated proteolysis of L1CAM in a mouse model of idiopathic normal pressure hydrocephalus
Published: 2021-08-11
Journal: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Application: WB
Impact Factor: 11.21
Species: Mouse,Human
PMID: 34380733
Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in recruitment of host fibronectin and plasminogen
Published: 2017-03-21
Journal: VETERINARY RESEARCH
Application: WB
Impact Factor: 2.80
Species: Erysipelothrix Rhusiopathiae
PMID: 28327178
Erysipelothrix rhusiopathiae recruits host plasminogen via the major protective antigen SpaA
Published: 2017-02-15
Journal: FEMS MICROBIOLOGY LETTERS
Application: WB
Impact Factor: 1.77
Species: Human
PMID: 28201685
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Product Promise
We promise Anti-Plasminogen/PLG Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03674-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026