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Product Info Summary
| SKU: | A00150S350 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SHP-2 (S350) PTPN11 Antibody
SKU/Catalog Number
A00150S350
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SHP-2 (S350) PTPN11 Antibody catalog # A00150S350. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHP-2 (S350) PTPN11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00150S350)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 320-370 of Human SHP-2.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00150S350 is reactive to PTPN11 in Human, Mouse, Rat
Calculated molecular weight
68.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00150S350 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of SHP-2 (K536) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of SHP-2 (S350) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
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Immunoprecipitation of L02 cell lysate using SHP-2 (S350) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using SHP-2 (S350) pAb #BS1323.“↑”（supernatant）; “↓”（deposition）
Specific Publications For Anti-SHP-2 (S350) PTPN11 Antibody (A00150S350)
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