This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
AKT1 antibody (P00024-5) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
Filter:
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Bundle Save
Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody
AKT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
AKT1 antibody was positively detected by IHC in:
human kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
ICC
IF
Human
Recommend
Tested
Recommend
Recommend
Mouse
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody
View all AKT1 Antibodies
View all AKT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IFD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Akt (Ser473)
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
55.7 kDa
Cite This Product
Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-5)
Protocols
Immunohistochemistry (IHC) protocol for AKT1 antibody
Publications
Application:
Species:
Bioinformatic and experimental analysis reveals ITGA5 as a driver of ovarian clear cell carcinoma progression and platinum resistance
Published: 2026-05-20
Journal: 3 Biotech
Application: WB
Impact Factor: 3.10
Species: Human
PMID: 42179928
Crocin Ameliorates Testicular Damage in Type 1 Diabetic Rats: Role of Akt-Mediated Nrf-2 Activation
Published: 2026-05-05
Journal: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
Application: WB
Impact Factor: 3.60
Species: Rat
PMID: 42085574
BEZ235 enhances the photodynamic antitumor efficacy of hypoxia-responsive BODIPY prodrug nanoparticles by inhibiting the PI3K/mTOR pathway and alleviating hypoxia
Published: 2026-03-22
Journal: COLLOIDS AND SURFACES B-BIOINTERFACES
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 41887152
Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis
Published: 2026-01-02
Journal: FITOTERAPIA
Application: WB
Impact Factor: 2.60
Species: Mouse
PMID: 41485690
Aloperine treatment attenuates acute spinal cord injury by reducing oxidative, inflammatory, and apoptotic responses via PI3K/AKT/NF-κB signaling in a rat contusion model
Published: 2025-03-23
Journal: NEUROSCIENCE LETTERS
Application: WB
Impact Factor: 2.00
Species: Rat
PMID: 40132779
Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis.
Published: 2024-09-19
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 6.70
Species: Human
PMID: 39341126
Study on the mechanism of aging-related erectile dysfunction based on bioinformatics and experimental verification
Published: 2023-02-03
Journal: Translational Andrology and Urology
Application: WB
Impact Factor: 2.00
Species: Rat
PMID: 36915879
Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells
Published: 2023-01-02
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.80
Species: Human
PMID: 36603687
Estradiol Regulates the Expression and Secretion of Antimicrobial Peptide S100A7 via the ERK1/2-Signaling Pathway in Goat Mammary Epithelial Cells
Published: 2022-11-08
Journal: Animals
Application: WB
Impact Factor: 3.23
Species: Goat
PMID: 36428305
Propionate ameliorates diabetes-induced neurological dysfunction through regulating the PI3K/Akt/eNOS signaling pathway
Published: 2022-04-28
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.20
Species: Mouse
PMID: 35490725
Effects of 4-vinylcyclohexene diepoxide on the cell cycle, apoptosis, and steroid hormone secretion of goat ovarian granulosa cells
Published: 2022-04-06
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: WB
Impact Factor: 2.72
Species: Goat
PMID: 35386089
Sesquiterpene lactones; Damsin and neoambrosin suppress cytokine-mediated inflammation in complete Freund's adjuvant rat model via shutting Akt/ERK1/2/STAT3 signaling
Published: 2020-09-23
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 3.69
Species: Rat
PMID: 32979413
Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3α Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs
Published: 2019-12-12
Journal: Marine Drugs
Application: WB,IF
Impact Factor: 3.77
Species: Rat
PMID: 31842414
Review
Have you used Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00024-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026