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Product Info Summary
| SKU: | P00024-5 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00024-5
BM4838 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody catalog # P00024-5. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-5)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IFD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Akt (Ser473)
Reactive Species
P00024-5 is reactive to AKT1 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
55.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00024-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
Positive Control
IHC: human kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using Phospho-Akt(Ser473) Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of NIH/3T3 cells treated with PDGF, using Phospho-Akt(Ser473) Antibody.
Specific Publications For Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody (P00024-5)
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4 Customer Q&As for Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody
Question
Our team were happy with the WB result of your anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody. However we have seen positive staining in muscle ovary cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Based on literature, muscle ovary does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-30
Question
We have been able to see staining in mouse left adrenal gland. Do you have any suggestions? Is anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody supposed to stain left adrenal gland positively?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
From literature left adrenal gland does express AKT1. From Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-26
Question
Our lab used your anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody for WB on muscle ovary a few months ago. I am using rat, and We intend to use the antibody for ICC next. We need examining muscle ovary as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
I have checked the website and datasheets of our anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody and it appears that P00024-5 has been tested on rat in both WB and ICC. Thus P00024-5 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-19
Question
We are currently using anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody P00024-5 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
L. Johnson
Verified customer
Asked: 2015-07-24
Answer
The anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody (P00024-5) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-24
