Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody

Boster Bio Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody catalog # P00024-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 20 publication(s).

Product Info Summary

SKU: P00024-5
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody

See all Akt1 products

SKU/Catalog Number

P00024-5

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody catalog # P00024-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-5)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IFD-1

Immunogen

A synthesized peptide derived from human Phospho-Akt (Ser473)

Reactive Species

P00024-5 is reactive to AKT1 in Human, Mouse, Rat

Applications

P00024-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500

Validation Images & Assay Conditions

Gene/Protein Information For AKT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P31749

Gene ID

207

Gene Name

AKT1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.686kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AKT1, check out the AKT1 Infographic

AKT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AKT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

P00024-5 has been cited in 20 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Güleç İ, Şengelen A,Karagöz-Güzey F,Önay-Uçar E,Eren B,Vahabova G,Karacan M,Bilgen Özcan T.The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model.Brain Res.2021 Apr 27:147508.doi:10.1016/j. brainres.2021. 147508.Epub ahead of print.PMID:33930376.
Species: Rat
P00024-5 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:500

Xu Y,Chen W,Chen Z,Huang M,Yang F,Zhang Y.Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway.Drug Des Devel Ther.2021 Feb 22;15:753-767.doi:10.2147/DDDT.S280217.PMID:33654381;
Species: Rat
P00024-5 usage in article: APP:WB, SAMPLE:PC12 CELL, DILUTION:1:500

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
P00024-5 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
P00024-5 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA

Xiao L,Zhang H,Yang X,Mahati S,Wu G,Xiaheding Y,Bao YX,Xiao H.Role of phosphatidylinositol 3-kinase signaling pathway in radiation-induced liver injury.Kaohsiung J Med Sci.2020 Jul 30.doi:10.1002/kjm2.12279. Epub ahead of print.PMID:32729224.
Species: Rat
P00024-5 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA

Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
Species: Rat
P00024-5 usage in article: APP:IF, SAMPLE:ADSCS, DILUTION:1:400

A small interfering RNA targeting osteopontin as gastric cancer therapeutics

Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells

Protective Effects of Sodium (?)-5-Bromo-2-(%u03B1-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia

Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice

Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway

Li R, Cui K, Liu K, Li H, Zhang Y, Liu X, Chen R, Li M, Wang T, Wang S, Liu J, Rao K. Sci Rep. 2017 Oct 18;7(1):13464. doi: 10.1038/s41598-017-12907-1. Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS a...

Wang Q, Sun X, Li X, Dong X, Li P, Zhao L. Mol Med Rep. 2015 Jan;11(1):151-8. Doi: 10.3892/Mmr.2014.2762. Epub 2014 Oct 23. Resveratrol Attenuates Intermittent Hypoxia-Induced Insulin Resistance In Rats: Involvement Of Sirtuin 1 And The Phosphatid...

Wan J, Che Y, Kang N, Wu W. Mol Med Rep. 2015 Jul;12(1):83-92. Doi: 10.3892/Mmr.2015.3368. Epub 2015 Feb 17. Socs3 Blocks Hif-1?? Expression To Inhibit Proliferation And Angiogenesis Of Human Small Cell Lung Cancer By Downregulating Activation Of ...

Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Cardiovasc Diabetol. 2013 Feb 26;12:37. Doi: 10.1186/1475-2840-12-37. Heparanase Induced By Advanced Glycation End Products (Ages) Promotes Macrophage Migration Involving Rage And Pi3K/Akt Pathway.

Wang Gg, Li W, Lu Xh, Zhao X, Xu L. Croat Med J. 2013 Apr;54(2):171-9. Taurine Attenuates Oxidative Stress And Alleviates Cardiac Failure In Type I Diabetic Rats.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

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4 Customer Q&As for Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody

Question

Our team were happy with the WB result of your anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody. However we have seen positive staining in muscle ovary cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

Based on literature, muscle ovary does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-30

Question

We have been able to see staining in mouse left adrenal gland. Do you have any suggestions? Is anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody supposed to stain left adrenal gland positively?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

From literature left adrenal gland does express AKT1. From Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-02-26

Question

Our lab used your anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody for WB on muscle ovary a few months ago. I am using rat, and We intend to use the antibody for ICC next. We need examining muscle ovary as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

I have checked the website and datasheets of our anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody and it appears that P00024-5 has been tested on rat in both WB and ICC. Thus P00024-5 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-19

Question

We are currently using anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody P00024-5 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

L. Johnson

Verified customer

Asked: 2015-07-24

Answer

The anti-Phospho-Akt (Ser473) Rabbit Monoclonal antibody (P00024-5) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-07-24

Size

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P00024-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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