Anti-Superoxide Dismutase 1/SOD1 Antibody

SOD1/Cu-Zn SOD antibody

Boster Bio Anti-Superoxide Dismutase 1/SOD1 Antibody catalog # PA1345. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 8 publication(s).

Product Info Summary

SKU: PA1345
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-Superoxide Dismutase 1/SOD1 Antibody

View all SOD1/Cu-Zn SOD Antibodies

SKU/Catalog Number

PA1345

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Superoxide Dismutase 1/SOD1 Antibody catalog # PA1345. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Superoxide Dismutase 1/SOD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1345)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1, different from the mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1345 is reactive to SOD1 in Human, Mouse, Rat

Applications

PA1345 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

18 kDa

Calculated molecular weight

15.936kDa

Background of SOD1/Cu-Zn SOD

Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For SOD1 (Source: Uniprot.org, NCBI)

Gene Name

SOD1

Full Name

Superoxide dismutase [Cu-Zn]

Weight

15.936kDa

Superfamily

Cu-Zn superoxide dismutase family

Alternative Names

ALS; ALS1; amyotrophic lateral sclerosis 1 (adult); Cu; Cu/Zn superoxide dismutase; CuZn SOD; Cu-Zn SOD; EC 1.15.1.1; homodimer; hSod1; indophenoloxidase A; Ipo1; IPOA; SOD; SOD, cytosolic; SOD, Soluble; SOD1; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic SOD1 ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer superoxide dismutase 1 superoxide dismutase [Cu-Zn]|Cu/Zn superoxide dismutase|SOD, soluble|epididymis secretory protein Li 44|indophenoloxidase A|superoxide dismutase 1, soluble|superoxide dismutase, cystolic

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SOD1, check out the SOD1 Infographic

SOD1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SOD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1345 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Akt specific activator SC79 protects against early brain injury following subarachnoid hemorrhage

The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway

Protective Role of Antioxidant Huskless Barley Extracts on TNF-?-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells

Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation

Guo Z, Qi W, Yu Y, Du S, Wu J, Liu J. Diabetol Metab Syndr. 2014 Feb 28;6(1):29. Doi: 10.1186/1758-5996-6-29. Effect Of Exenatide On The Cardiac Expression Of Adiponectin Receptor 1 And Nadph Oxidase Subunits And Heart Function In Streptozotocin-I...

Hou S, Zheng F, Li Y, Gao L, Zhang J. Int J Mol Sci. 2014 Aug 26;15(9):15026-43. Doi: 10.3390/Ijms150915026. The Protective Effect Of Glycyrrhizic Acid On Renal Tubular Epithelial Cell Injury Induced By High Glucose.

Long Sh, Yu Zq, Shuai L, Guo Yl, Duan Dl, Xu Xy, Li Xd. Int J Mol Sci. 2012;13(3):3354-65. Doi: 10.3390/Ijms13033354. Epub 2012 Mar 12. The Hypoglycemic Effect Of The Kelp On Diabetes Mellitus Model Induced By Alloxan In Rats.

Chen J, Chen X, Qin J. Brain Inj. 2011;25(3):292-9. Doi: 10.3109/02699052.2010.546824. Epub 2011 Jan 31. Effects Of Polysaccharides Of The Euphoria Longan (Lour.) Steud On Focal Cerebral Ischemia/Reperfusion Injury And Its Underlying Mechanism.

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18 Customer Q&As for Anti-Superoxide Dismutase 1/SOD1 Antibody

Question

Can you please advise whether your lab might be able to provide PA1345 antibody in a BSA-free buffer?

Verified customer

Asked: 2020-06-23

Answer

Yes, we can provide the Anti-Superoxide Dismutase 1/SOD1 Antibody (PA1345) in a BSA-free buffer. It will take 3-4 months to produce it and we require a minimum order of 1mg.

Boster Scientific Support

Answered: 2020-06-23

Question

I am interested in to test anti-Superoxide Dismutase 1/SOD1 antibody PA1345 on human fetal brain cortex for research purposes, then I may be interested in using anti-Superoxide Dismutase 1/SOD1 antibody PA1345 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

The products we sell, including anti-Superoxide Dismutase 1/SOD1 antibody PA1345, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-31

Question

Our lab used your anti-Superoxide Dismutase 1/SOD1 antibody for IHC on cervix carcinoma last year. I am using mouse, and We are going to use the antibody for ICC next. you antibody examining cervix carcinoma as well as pons in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

I took a look at the website and datasheets of our anti-Superoxide Dismutase 1/SOD1 antibody and it seems that PA1345 has been validated on mouse in both IHC and ICC. Thus PA1345 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-18

Question

Would anti-Superoxide Dismutase 1/SOD1 antibody PA1345 work for IHC with fetal brain cortex?

E. Anderson

Verified customer

Asked: 2020-03-18

Answer

According to the expression profile of fetal brain cortex, SOD1 is highly expressed in fetal brain cortex. So, it is likely that anti-Superoxide Dismutase 1/SOD1 antibody PA1345 will work for IHC with fetal brain cortex.

Boster Scientific Support

Answered: 2020-03-18

Question

Does anti-Superoxide Dismutase 1/SOD1 antibody PA1345 work on pig ICC with fetal brain cortex?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

Our lab technicians have not tested anti-Superoxide Dismutase 1/SOD1 antibody PA1345 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Superoxide Dismutase 1/SOD1 antibody PA1345 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig fetal brain cortex in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-24

Question

I was wanting to use your anti-Superoxide Dismutase 1/SOD1 antibody for IHC for human fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain cortex identification?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

It shows on the product datasheet, PA1345 anti-Superoxide Dismutase 1/SOD1 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-04

Question

We have seen staining in human liver. Any tips? Is anti-Superoxide Dismutase 1/SOD1 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

From literature liver does express SOD1. From Uniprot.org, SOD1 is expressed in pons, colon, placenta, fetal brain cortex, cervix carcinoma, liver, among other tissues. Regarding which tissues have SOD1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Colon, Pubmed ID: 14702039
Fetal brain cortex, Pubmed ID: 8528216
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-13

Question

My question regarding product PA1345, anti-Superoxide Dismutase 1/SOD1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1345 anti-Superoxide Dismutase 1/SOD1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-21

Question

Is there a BSA free version of anti-Superoxide Dismutase 1/SOD1 antibody PA1345 available?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Superoxide Dismutase 1/SOD1 antibody PA1345 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-28

Question

I see that the anti-Superoxide Dismutase 1/SOD1 antibody PA1345 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-24

Question

Is a blocking peptide available for product anti-Superoxide Dismutase 1/SOD1 antibody (PA1345)?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

We do provide the blocking peptide for product anti-Superoxide Dismutase 1/SOD1 antibody (PA1345). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-13

Question

Will PA1345 anti-Superoxide Dismutase 1/SOD1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

You can see on the product datasheet, PA1345 anti-Superoxide Dismutase 1/SOD1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-29

Question

Is this PA1345 anti-Superoxide Dismutase 1/SOD1 antibody reactive to the isotypes of SOD1?

T. Parker

Verified customer

Asked: 2018-09-27

Answer

The immunogen of PA1345 anti-Superoxide Dismutase 1/SOD1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1 (113-129aa IIGRTLVVHEKADDLGK), different from the mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-27

Question

See below the WB image, lot number and protocol we used for fetal brain cortex using anti-Superoxide Dismutase 1/SOD1 antibody PA1345. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-03

Question

We want using your anti-Superoxide Dismutase 1/SOD1 antibody for response to organic substance studies. Has this antibody been tested with western blotting on colo320 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-03-09

Answer

Thanks for your inquiry. This PA1345 anti-Superoxide Dismutase 1/SOD1 antibody is tested on colo320 cell lysate, smmc cell lysate, mammary cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-03-09

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-Superoxide Dismutase 1/SOD1 antibody PA1345. Let me know if you need anything else.

T. Zhang

Verified customer

Asked: 2016-02-17

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-02-17

Question

We are currently using anti-Superoxide Dismutase 1/SOD1 antibody PA1345 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

E. Walker

Verified customer

Asked: 2015-09-15

Answer

The anti-Superoxide Dismutase 1/SOD1 antibody (PA1345) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-09-15

Question

My team were content with the WB result of your anti-Superoxide Dismutase 1/SOD1 antibody. However we have been able to see positive staining in pons cytoplasm. using this antibody. Is that expected? Could you tell me where is SOD1 supposed to be expressed?

C. Moore

Verified customer

Asked: 2015-03-19

Answer

Based on literature, pons does express SOD1. Generally SOD1 expresses in cytoplasm. Regarding which tissues have SOD1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Colon, Pubmed ID: 14702039
Fetal brain cortex, Pubmed ID: 8528216
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-03-19

Order DetailsPrice
PA1345

100μg

$370
PA1345-10ug

10μg sample (liquid)

$99
PA1345-Biotin

100 μg Biotin conjugated

$570
PA1345-Cy3

100 μg Cy3 conjugated

$570
PA1345-Dylight488

100 μg Dylight488 conjugated

$570
PA1345-Dylight550

100 μg Dylight550 conjugated

$570
PA1345-Dylight594

100 μg Dylight594 conjugated

$570
PA1345-FITC

100 μg FITC conjugated

$570
PA1345-HRP

100 μg HRP conjugated

$570
PA1345-APC

100 μg APC conjugated

$670
PA1345-PE

100 μg PE conjugated

$670
PA1345-carrier-free

Carrier Free

$370

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PA1345
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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