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- Table of Contents
ATP1A1 antibody (M00956) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody
ATP1A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ATP1A1 antibody was positively detected by IHC in:
Rat cerebral cortex tissue, Rat heart tissue, Human cervical cancer tissue, Human thyroid cancer tissue, Mouse skeletal muscle - gastrocnemius tissue, human thyroid carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Rat
Recommend
Tested
Recommend
Recommend
Recommend
Human
Recommend
Tested
Recommend
Recommend
Recommend
Mouse
Recommend
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody
View all ATP1A1 Antibodies
View all ATP1A1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AID-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Sodium Potassium ATPase
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
112.9 kDa
Cite This Product
Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00956)
Protocols
Immunohistochemistry (IHC) protocol for ATP1A1 antibody
Publications
Application:
Species:
A self-amplifying cuproptosis nanomedicine to overcome immunosuppression by blocking tumor-derived exosomes for enhancing lung cancer immunotherapy
Published: 2026-01-02
Journal: JOURNAL OF CONTROLLED RELEASE
Application: WB
Impact Factor: 11.50
Species: Mouse
PMID: 41485488
Review
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1 Review For Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody
WB analysis using Anti-ATP1A1 antibody (M00956) in HepG2 cells showed a clear and specific band at the expected molecular weight, with minimal background, demonstrating excellent antibody performance.
| SKU | M00956 |
|---|---|
| Application | Western Blot |
| Sample | human adrenocortical carcinoma tissue |
| Sample Processing Description | Total protein was extracted from clinically collected human adrenocortical carcinoma tissue. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:5000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1h in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | ATP1A1 encodes the α1 catalytic subunit of the Na⁺/K⁺-ATPase, whose core physiological function is to maintain the transmembrane sodium and potassium electrochemical gradient, serving as the basis for nearly all vital processes such as neuronal excitability, renal reabsorption, and muscle contraction. In this study, normally cultured HepG2 cells (without any drug treatment) were used to validate the quality of the ATP1A1 antibody. The results showed a clear target band at the correct position with clean background, indicating that the antibody performs well. |
Yumeng Han, Nanjing Medical University
Verified customer
Submitted 2026-05-06
Product Promise
We promise Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00956
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026