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Product Info Summary
| SKU: | M00956 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00956
BM4048 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody catalog # M00956. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00956)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AID-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Sodium Potassium ATPase
Reactive Species
M00956 is reactive to ATP1A1 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
112.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00956 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
IHC: Rat cerebral cortex tissue, Rat heart tissue, Human cervical cancer tissue, Human thyroid cancer tissue, Mouse skeletal muscle - gastrocnemius tissue, human thyroid carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using Sodium Potassium ATPase Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of ATP1A1 using anti-ATP1A1 antibody (M00956).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A1 antigen affinity purified monoclonal antibody (M00956) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for ATP1A1 at approximately 113 kDa. The expected band size for ATP1A1 is at 113 kDa.
Specific Publications For Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody (M00956)
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Customer Reviews
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1 Reviews For Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody
WB analysis using Anti-ATP1A1 antibody (M00956) in HepG2 cells showed a clear and specific band at the expected molecular weight, with minimal background, demonstrating excellent antibody performance.
Excellent
| SKU | M00956 |
|---|---|
| Application | Western Blot |
| Sample | human adrenocortical carcinoma tissue |
| Sample Processing Description | Total protein was extracted from clinically collected human adrenocortical carcinoma tissue. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:5000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1h in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | ATP1A1 encodes the α1 catalytic subunit of the Na⁺/K⁺-ATPase, whose core physiological function is to maintain the transmembrane sodium and potassium electrochemical gradient, serving as the basis for nearly all vital processes such as neuronal excitability, renal reabsorption, and muscle contraction. In this study, normally cultured HepG2 cells (without any drug treatment) were used to validate the quality of the ATP1A1 antibody. The results showed a clear target band at the correct position with clean background, indicating that the antibody performs well. |
Yumeng Han, Nanjing Medical University
Verified customer
Submitted 2026-05-06
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