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- Table of Contents
Txn antibody (A01219-1) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).
Thioredoxin TRX was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thioredoxin TRX Antibody (A01219-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Thioredoxin TRX was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thioredoxin TRX Antibody (A01219-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-Thioredoxin/TRX/Txn Antibody Picoband®
Txn antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
Txn antibody was positively detected by WB in:
rat kidney tissue, rat small intestine tissue, rat spleen tissue, rat lung tissue, rat C6 whole cell, mouse kidney tissue, mouse small intestine tissue, mouse lung tissue, mouse heart tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Txn antibody was positively detected by IHC in:
mouse small intestine tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Txn antibody was positively detected by ICC/IF in:
NIH3T3 cell
Txn antibody was positively detected by ICC in:
LLC Cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Thioredoxin/TRX/Txn Antibody Picoband®
View all TXN Antibodies
View all TXN Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
12 kDa
Calculated Molecular Weight
11.7 kDa
Background of Txn
Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-Thioredoxin/TRX/Txn Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01219-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
98%
High Homology
Human
88%
Moderate Homology
Cow
88%
Moderate Homology
Dog
88%
Moderate Homology
Monkey
88%
Moderate Homology
Sheep
87%
Moderate Homology
Rabbit
83%
Moderate Homology
Zebrafish
63%
Low homology
Drosophila
49%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Txn antibody
Immunohistochemistry (IHC) protocol for Txn antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Txn antibody
Immunocytochemistry (ICC) protocol for Txn antibody
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Product Promise
We promise Anti-Thioredoxin/TRX/Txn Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01219-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026