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Product Info Summary
| SKU: | M01116 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-SPI1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01116
BM5630 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SPI1 Rabbit Monoclonal Antibody catalog # M01116. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPI1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01116)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEIG-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PU.1/Spi1
Reactive Species
M01116 is reactive to SPI1 in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
31.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01116 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:60
Positive Control
WB: human HEL whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SPI1 using anti-SPI1 antibody (M01116).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPI1 antigen affinity purified monoclonal antibody (M01116) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPI1 at approximately 40 kDa. The expected band size for SPI1 is at 31 kDa.
Specific Publications For Anti-SPI1 Rabbit Monoclonal Antibody (M01116)
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Customer Q&As
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5 Customer Q&As for Anti-SPI1 Rabbit Monoclonal Antibody
Question
What sample was used in your WB analysis of M01116?
Verified customer
Asked: 2019-12-18
Answer
For the Anti-SPI1 Rabbit Monoclonal Antibody (M01116), western blot analysis of PU.1/Spi1 expression in Daudi cell lysate.
Boster Scientific Support
Answered: 2019-12-20
Question
I see that the anti-SPI1 Monoclonal antibody M01116 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-25
Question
Is this M01116 anti-SPI1 Monoclonal antibody reactive to the isotypes of SPI1?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
The immunogen of M01116 anti-SPI1 Monoclonal antibody is A synthesized peptide derived from human PU.1/Spi1 Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-10
Question
Can you help my question with product M01116, anti-SPI1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01116 anti-SPI1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-17
Question
See below the WB image, lot number and protocol we used for blood using anti-SPI1 Monoclonal antibody M01116. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-23
