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Product Info Summary
| SKU: | PA1608 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hsp105/HSPH1 Antibody Picoband®
SKU/Catalog Number
PA1608
BA0931 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hsp105/HSPH1 Antibody catalog # PA1608. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp105/HSPH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1608)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105, different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1608 is reactive to HSPH1 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
96.9 kDa
Background of HSPH1
HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1608 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Ovary Tissue, A549 Cell, U87 Cell, HELA Cell,
IHC: Human Mammary Cancer Tissue, Rat Intestine Tissue
IHC-F: Rat Intestine Tissue
ICC/IF: A549 cell
FCM: A431 cell
Validation Images & Assay Conditions
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Anti-Hsp105 antibody, PA1608, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
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Anti-Hsp105 antibody, PA1608, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Hsp105 antibody, PA1608, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-Hsp105 antibody, PA1608, IHC(F)
IHC(F): Rat Intestine Tissue
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IF analysis of HSPH1 using anti-HSPH1 antibody (PA1608).
HSPH1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HSPH1 Antibody (PA1608) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of A431 cells using anti-HSPH1 antibody (PA1608).
Overlay histogram showing A431 cells stained with PA1608 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPH1 Antibody (PA1608, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Hsp105/HSPH1 Antibody Picoband® (PA1608)
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Customer Q&As
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6 Customer Q&As for Anti-Hsp105/HSPH1 Antibody Picoband®
Question
Would PA1608 anti-Hsp105/HSPH1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
As indicated on the product datasheet, PA1608 anti-Hsp105/HSPH1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-14
Question
Is a blocking peptide available for product anti-Hsp105/HSPH1 antibody (PA1608)?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We do provide the blocking peptide for product anti-Hsp105/HSPH1 antibody (PA1608). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-06
Question
Is this PA1608 anti-Hsp105/HSPH1 antibody reactive to the isotypes of HSPH1?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The immunogen of PA1608 anti-Hsp105/HSPH1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105(713-733aa EVMEWMNNVMNAQAKKSLDQD), different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-12
Question
We are currently using anti-Hsp105/HSPH1 antibody PA1608 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
E. Miller
Verified customer
Asked: 2018-12-20
Answer
The anti-Hsp105/HSPH1 antibody (PA1608) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-20
Question
Do you have a BSA free version of anti-Hsp105/HSPH1 antibody PA1608 available?
A. Jha
Verified customer
Asked: 2018-10-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp105/HSPH1 antibody PA1608 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-01
Question
I see that the anti-Hsp105/HSPH1 antibody PA1608 works with IHC, what is the protocol used to produce the result images on the product page?
S. Jones
Verified customer
Asked: 2018-01-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-01-31
