Anti-Hsp105/HSPH1 Antibody

HSPH1/HSP105 antibody

Boster Bio Anti-Hsp105/HSPH1 Antibody catalog # PA1608. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1608
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-Hsp105/HSPH1 Antibody

View all HSPH1/HSP105 Antibodies

SKU/Catalog Number

PA1608

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsp105/HSPH1 Antibody catalog # PA1608. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp105/HSPH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1608)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105, different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1608 is reactive to HSPH1 in Human, Mouse, Rat

Applications

PA1608 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

96.865kDa

Background of HSPH1/HSP105

HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For HSPH1 (Source: Uniprot.org, NCBI)

Gene Name

HSPH1

Full Name

Heat shock protein 105 kDa

Weight

96.865kDa

Superfamily

heat shock protein 70 family

Alternative Names

Antigen NY-CO-25; heat shock 105kD beta; heat shock 105kDa protein 1; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; heat shock protein 105 kDa; HSP105a; HSP105B; HSP105heat shock 105kD alpha; HSP110; HSPH1; KIAA0201DKFZp686M05240; NY-CO-25 HSPH1 HSP105, HSP105A, HSP105B, NY-CO-25 heat shock protein family H (Hsp110) member 1 heat shock protein 105 kDa|antigen NY-CO-25|heat shock 105kDa/110kDa protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPH1, check out the HSPH1 Infographic

HSPH1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Hsp105/HSPH1 Antibody

Question

Would PA1608 anti-Hsp105/HSPH1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

As indicated on the product datasheet, PA1608 anti-Hsp105/HSPH1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-14

Question

Is a blocking peptide available for product anti-Hsp105/HSPH1 antibody (PA1608)?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

We do provide the blocking peptide for product anti-Hsp105/HSPH1 antibody (PA1608). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-06

Question

Is this PA1608 anti-Hsp105/HSPH1 antibody reactive to the isotypes of HSPH1?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

The immunogen of PA1608 anti-Hsp105/HSPH1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105(713-733aa EVMEWMNNVMNAQAKKSLDQD), different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-12

Question

We are currently using anti-Hsp105/HSPH1 antibody PA1608 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

E. Miller

Verified customer

Asked: 2018-12-20

Answer

The anti-Hsp105/HSPH1 antibody (PA1608) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-12-20

Question

Do you have a BSA free version of anti-Hsp105/HSPH1 antibody PA1608 available?

A. Jha

Verified customer

Asked: 2018-10-01

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp105/HSPH1 antibody PA1608 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-10-01

Question

I see that the anti-Hsp105/HSPH1 antibody PA1608 works with IHC, what is the protocol used to produce the result images on the product page?

S. Jones

Verified customer

Asked: 2018-01-31

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-31

Order DetailsPrice
PA1608

100μg

$370
PA1608-10ug

10μg sample (liquid)

$99
PA1608-Biotin

100 μg Biotin conjugated

$570
PA1608-Cy3

100 μg Cy3 conjugated

$570
PA1608-Dylight488

100 μg Dylight488 conjugated

$570
PA1608-Dylight550

100 μg Dylight550 conjugated

$570
PA1608-Dylight594

100 μg Dylight594 conjugated

$570
PA1608-FITC

100 μg FITC conjugated

$570
PA1608-HRP

100 μg HRP conjugated

$570
PA1608-APC

100 μg APC conjugated

$670
PA1608-PE

100 μg PE conjugated

$670
PA1608-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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