Product Info Summary
SKU: | PA1608 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hsp105/HSPH1 Antibody
View all HSPH1/HSP105 Antibodies
SKU/Catalog Number
PA1608
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hsp105/HSPH1 Antibody catalog # PA1608. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp105/HSPH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1608)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105, different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1608 is reactive to HSPH1 in Human, Mouse, Rat
Applications
PA1608 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
96.865kDa
Background of HSPH1/HSP105
HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Hsp105 antibody, PA1608, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Click image to see more details
Anti-Hsp105 antibody, PA1608, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Click image to see more details
Anti-Hsp105 antibody, PA1608, IHC(P)
IHC(P): Rat Intestine Tissue
Click image to see more details
Anti-Hsp105 antibody, PA1608, IHC(F)
IHC(F): Rat Intestine Tissue
Click image to see more details
Figure 5. IF analysis of HSPH1 using anti-HSPH1 antibody (PA1608).
HSPH1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HSPH1 Antibody (PA1608) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of A431 cells using anti-HSPH1 antibody (PA1608).
Overlay histogram showing A431 cells stained with PA1608 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPH1 Antibody (PA1608, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For HSPH1 (Source: Uniprot.org, NCBI)
Gene Name
HSPH1
Full Name
Heat shock protein 105 kDa
Weight
96.865kDa
Superfamily
heat shock protein 70 family
Alternative Names
Antigen NY-CO-25; heat shock 105kD beta; heat shock 105kDa protein 1; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; heat shock protein 105 kDa; HSP105a; HSP105B; HSP105heat shock 105kD alpha; HSP110; HSPH1; KIAA0201DKFZp686M05240; NY-CO-25 HSPH1 HSP105, HSP105A, HSP105B, NY-CO-25 heat shock protein family H (Hsp110) member 1 heat shock protein 105 kDa|antigen NY-CO-25|heat shock 105kDa/110kDa protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPH1, check out the HSPH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp105/HSPH1 Antibody (PA1608)
Hello CJ!
No publications found for PA1608
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hsp105/HSPH1 Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Hsp105/HSPH1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-Hsp105/HSPH1 Antibody
Question
Would PA1608 anti-Hsp105/HSPH1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
As indicated on the product datasheet, PA1608 anti-Hsp105/HSPH1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-14
Question
Is a blocking peptide available for product anti-Hsp105/HSPH1 antibody (PA1608)?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We do provide the blocking peptide for product anti-Hsp105/HSPH1 antibody (PA1608). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-06
Question
Is this PA1608 anti-Hsp105/HSPH1 antibody reactive to the isotypes of HSPH1?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The immunogen of PA1608 anti-Hsp105/HSPH1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105(713-733aa EVMEWMNNVMNAQAKKSLDQD), different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-12
Question
We are currently using anti-Hsp105/HSPH1 antibody PA1608 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
E. Miller
Verified customer
Asked: 2018-12-20
Answer
The anti-Hsp105/HSPH1 antibody (PA1608) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-20
Question
Do you have a BSA free version of anti-Hsp105/HSPH1 antibody PA1608 available?
A. Jha
Verified customer
Asked: 2018-10-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp105/HSPH1 antibody PA1608 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-01
Question
I see that the anti-Hsp105/HSPH1 antibody PA1608 works with IHC, what is the protocol used to produce the result images on the product page?
S. Jones
Verified customer
Asked: 2018-01-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-31