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- Table of Contents
BIRC5 antibody (A00379-1) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunohistochemistry Validation of Survivin in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Survivin antibody (A00379-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Survivin antibody (A00379-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Anti-Survivin BIRC5 Antibody
BIRC5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-5μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Survivin BIRC5 Antibody
View all BIRC5 Antibodies
View all BIRC5 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Survivin antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
Survivin Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Survivin antibody was raised against a peptide corresponding to 12 amino acids near the amino terminus of human Survivin. The immunogen is located within the first 50 amino acids of Survivin.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
16.4 kDa
Background of BIRC5
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
Cite This Product
Anti-Survivin BIRC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00379-1)
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Product Promise
We promise Anti-Survivin BIRC5 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00379-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026