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Product Info Summary
| SKU: | PB9902 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TRPM4 Antibody Picoband®
SKU/Catalog Number
PB9902
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPM4 Antibody Picoband® catalog # PB9902. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPM4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9902)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPM4 recombinant protein (Position: F1079-D1214). Human TRPM4 shares 76.8% and 77.8% amino acid (aa) sequence identity with mouse and rat TRPM4, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9902 is reactive to TRPM4 in Human
Observed Molecular Weight
167 kDa
Calculated molecular weight
134.3 kDa
Background of TRPM4
Transient receptor potential cation channel subfamily M member 4 (hTRPM4), also known as melastatin-4, is a protein that in humans is encoded by the TRPM4 gene. It is mapped to 19q13.33. The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9902 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human Hela whole cell
IHC: human prostate cancer tissue, human squamous cell carcinoma of cervix tissue
Validation Images & Assay Conditions
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Western blot analysis of TRPM4 using anti-TRPM4 antibody (PB9902).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM4 antigen affinity purified polyclonal antibody (Catalog # PB9902) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM4 at approximately 167 kDa. The expected band size for TRPM4 is at 134 kDa.
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IHC analysis of TRPM4 using anti-TRPM4 antibody (PB9902).
TRPM4 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRPM4 Antibody (PB9902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of TRPM4 using anti-TRPM4 antibody (PB9902).
TRPM4 was detected in a paraffin-embedded section of human squamous cell carcinoma of cervix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRPM4 Antibody (PB9902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-TRPM4 Antibody Picoband® (PB9902)
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6 Customer Q&As for Anti-TRPM4 Antibody Picoband®
Question
Can you help my question with product PB9902, anti-TRPM4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9902 anti-TRPM4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-01
Question
We are currently using anti-TRPM4 antibody PB9902 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The anti-TRPM4 antibody (PB9902) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-25
Question
I am interested in to test anti-TRPM4 antibody PB9902 on human trachea for research purposes, then I may be interested in using anti-TRPM4 antibody PB9902 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The products we sell, including anti-TRPM4 antibody PB9902, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-15
Question
Is this PB9902 anti-TRPM4 antibody reactive to the isotypes of TRPM4?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
The immunogen of PB9902 anti-TRPM4 antibody is E.coli-derived human TRPM4 recombinant protein (Position: F1079-D1214). Human TRPM4 shares 76.8% and 77.8% amino acid (aa) sequence identity with mouse and rat TRPM4, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-27
Question
I see that the anti-TRPM4 antibody PB9902 works with WB, what is the protocol used to produce the result images on the product page?
K. Anderson
Verified customer
Asked: 2014-07-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-07-07
Question
Is there a BSA free version of anti-TRPM4 antibody PB9902 available?
D. Williams
Verified customer
Asked: 2013-02-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TRPM4 antibody PB9902 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-20
