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Product Info Summary
| SKU: | A05280-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CHRNA9 Antibody (N-term)
SKU/Catalog Number
A05280-1
Size
80 µl
Description
Boster Bio Anti-CHRNA9 Antibody (N-term) (Catalog # A05280-1). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CHRNA9 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05280-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CHRNA9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 8-42 amino acids from the N-terminal region of human CHRNA9.
Reactive Species
A05280-1 is reactive to CHRNA9 in Human
Calculated molecular weight
54.8 kDa
Background of CHRNA9
Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05280-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from Daudi cell line, using CHRNA9 Antibody (N-term). A05280-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Flow cytometric analysis of Jurkat cells using CHRNA9 Antibody (N-term) (green, Cat#A05280-1) compared to an isotype control of rabbit IgG (blue). A05280-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-CHRNA9 Antibody (N-term) (A05280-1)
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