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Product Info Summary
| SKU: | A05280-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CHRNA9 Antibody (N-term)
View all Nicotinic Acetylcholine R alpha 9/CHRNA9 Antibodies
SKU/Catalog Number
A05280-1
Size
80 µl
Description
Boster Bio Anti-CHRNA9 Antibody (N-term) (Catalog # A05280-1). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CHRNA9 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05280-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CHRNA9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 8-42 amino acids from the N-terminal region of human CHRNA9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05280-1 is reactive to CHRNA9 in Human
Reconstitution
Calculated molecular weight
54807 Da
Background of Nicotinic Acetylcholine R alpha 9/CHRNA9
Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05280-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from Daudi cell line, using CHRNA9 Antibody (N-term). A05280-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Flow cytometric analysis of Jurkat cells using CHRNA9 Antibody (N-term) (green, Cat#A05280-1) compared to an isotype control of rabbit IgG (blue). A05280-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For CHRNA9 (Source: Uniprot.org, NCBI)
Gene Name
CHRNA9
Full Name
Neuronal acetylcholine receptor subunit alpha-9
Weight
54807 Da
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
Neuronal acetylcholine receptor subunit alpha-9, Nicotinic acetylcholine receptor subunit alpha-9, NACHR alpha-9, CHRNA9, NACHRA9 CHRNA9 HSA243342, NACHRA9 cholinergic receptor nicotinic alpha 9 subunit neuronal acetylcholine receptor subunit alpha-9|NACHR alpha 9|acetylcholine receptor, neuronal nicotinic, alpha-9 subunit|cholinergic receptor, nicotinic alpha 9|cholinergic receptor, nicotinic, alpha 9 (neuronal)|cholinergic receptor, nicotinic, alpha polypeptide 9|neuronal acetylcholine receptor protein, alpha-9 subunit|nicotinic acetylcholine receptor subunit alpha 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHRNA9, check out the CHRNA9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHRNA9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHRNA9 Antibody (N-term) (A05280-1)
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