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Product Info Summary
| SKU: | PB9829 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband®
SKU/Catalog Number
PB9829
PB0873 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband® catalog # PB9829. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9829)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TGFBR2, different from the related mouse sequence by five amino acids, and from the related rat sequence by eight amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9829 is reactive to TGFBR2 in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
64.6 kDa
Background of TGFBR2
TGFBR2 (transforming growth factor, beta receptor II (70/80kDa)), also known as TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II ( TGF-beta receptor type II, TbetaR-II), is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. A TGFBR2 cDNA encodes a deduced 565-amino acid protein with a calculated molecular mass of approximately 60 kD in length. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different informs have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9829 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human HepG2 whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TGFBR2 using anti-TGFBR2 antibody (PB9829).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFBR2 antigen affinity purified polyclonal antibody (Catalog # PB9829) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFBR2 at approximately 70 kDa. The expected band size for TGFBR2 is at 65 kDa.
Specific Publications For Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband® (PB9829)
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4 Customer Q&As for Anti-TGF beta Receptor II/TGFBR2 Antibody Picoband®
Question
We have seen staining in human placenta. Do you have any suggestions? Is anti-TGF beta Receptor II/TGFBR2 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Based on literature placenta does express TGFBR2. Based on Uniprot.org, TGFBR2 is expressed in metanephric glomerulus, liver, glial cell, placenta, cervix carcinoma, among other tissues. Regarding which tissues have TGFBR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 23186163
Glial cell, Pubmed ID: 7959019
Liver, Pubmed ID: 1310899, 8973329, 24275569
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-09
Question
Our lab were content with the WB result of your anti-TGF beta Receptor II/TGFBR2 antibody. However we have observed positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is TGFBR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
Based on literature, placenta does express TGFBR2. Generally TGFBR2 expresses in cell membrane. Regarding which tissues have TGFBR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 23186163
Glial cell, Pubmed ID: 7959019
Liver, Pubmed ID: 1310899, 8973329, 24275569
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-02-19
Question
We want using your anti-TGF beta Receptor II/TGFBR2 antibody for lens fiber cell apoptotic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
R. Wu
Verified customer
Asked: 2015-08-28
Answer
We appreciate your inquiry. This PB9829 anti-TGF beta Receptor II/TGFBR2 antibody is tested on sw620 whole cell lysate, 22rv1 whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-08-28
Question
We are currently using anti-TGF beta Receptor II/TGFBR2 antibody PB9829 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
G. Rodriguez
Verified customer
Asked: 2015-02-16
Answer
The anti-TGF beta Receptor II/TGFBR2 antibody (PB9829) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-16
