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Product Info Summary
| SKU: | A10056 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TIMM50 Antibody (N-term)
SKU/Catalog Number
A10056
Size
80 µl
Description
Boster Bio Anti-TIMM50 Antibody (N-term) (Catalog # A10056). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TIMM50 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10056)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TIMM50 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-56 amino acids from the N-terminal region of human TIMM50.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10056 is reactive to TIMM50 in Human
Reconstitution
Calculated molecular weight
39646 Da
Background of Timm50
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10056 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot analysis of lysate from human heart tissue lysate, using TIMM50 Antibody (N-term). A10056 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Western blot analysis of lysates from Hela, HepG2, SH-SY5Y cell line (from left to right), using TIMM50 Antibody (N-term) (Cat. #A10056). A10056 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Protein Target Info & Infographic
Gene/Protein Information For TIMM50 (Source: Uniprot.org, NCBI)
Gene Name
TIMM50
Full Name
Mitochondrial import inner membrane translocase subunit TIM50
Weight
39646 Da
Superfamily
TIM50 family
Alternative Names
Mitochondrial import inner membrane translocase subunit TIM50, TIMM50, TIM50
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TIMM50, check out the TIMM50 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TIMM50: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIMM50 Antibody (N-term) (A10056)
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