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- Table of Contents
DNM1L antibody (A00556-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of A549 cells using anti-DRP1/DNM1L antibody (A00556-2).
Overlay histogram showing A549 cells stained with A00556-2 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRP1/DNM1L Antibody (A00556-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A549 cells stained with A00556-2 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRP1/DNM1L Antibody (A00556-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-DRP1/DNM1L Antibody Picoband®
DNM1L antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
DNM1L antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, human Caco-2 whole cell, human K562 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DNM1L antibody was positively detected by IHC in:
human cerebellum tissue, human tonsil tissue, human liver cancer tissue, human appendicitis tissue, human bladder cancer tissue, human gastric cancer tissue, human lung cancer tissue, human melanoma tissue, human skin cancer tissue, human testicular cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
DNM1L antibody was positively detected by ICC/IF in:
U20S cell
DNM1L antibody was positively detected by FC in:
A549 cell, SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Mouse
Recommend
Rat
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-DRP1/DNM1L Antibody Picoband®
View all DNM1L Antibodies
View all DNM1L Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DRP1/DNM1L recombinant protein (Position: Q618-W736).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70-75 kDa
Calculated Molecular Weight
81.9 kDa
Background of DNM1L
Dynamin-1-like protein is a GTPase that regulates mitochondrial fission. In humans, dynamin-1-like protein, which is typically referred to as dynamin-related protein 1 (Drp1), is encoded by the DNM1L gene. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-DRP1/DNM1L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00556-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
95%
High Homology
Drosophila
68%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DNM1L antibody
Immunohistochemistry (IHC) protocol for DNM1L antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for DNM1L antibody
Flow Cytometry (FC) protocol for DNM1L antibody
Publications
Application:
Species:
Prolonged sevoflurane exposure induces exaggerated and persistent cognitive decline in old rats with pre-existing impairment by increasing hippocampal mitochondrial dysfunction and apoptosis
Published: 2026-07-31
Journal: NEUROTOXICOLOGY
Application: IHC
Impact Factor: 3.80
Species: Rat
PMID: 42537810
Promoting Drp1-Mediated Mitochondrial Division in Nickel Nanoparticles-Induced Reproductive Toxicity in GC-2 Cells
Published: 2025-12-25
Journal: Nanomaterials
Application: WB
Impact Factor: 4.30
Species: Mouse
PMID: 41511229
GPR75 knockdown alleviates mitochondrial dysfunction in hippocampal neurons via AMPK pathway in diabetic mice
Published: 2025-10-02
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 41043317
Young small extracellular vesicles rejuvenate replicative senescence by remodeling Drp1 translocation-mediated mitochondrial dynamics
Published: 2024-09-05
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: IF,WB
Impact Factor: 10.60
Species: Human
PMID: 39238005
Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study
Published: 2024-04-17
Journal: Nanomaterials
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 38668183
Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control
Published: 2023-05-10
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 37234709
Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow
Published: 2023-02-05
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 7.20
Species: Rat
PMID: 36740450
Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model
Published: 2022-06-08
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 3.61
Species: Rat
PMID: 35690143
Nicorandil, an ATP-sensitive potassium channel activation, attenuates myocardial injury in rats with ischemic cardiomyopathy
Published: 2021-11-13
Journal: Medical Molecular Morphology
Application: WB
Impact Factor: 2.31
Species: Rat
PMID: 34773514
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Product Promise
We promise Anti-DRP1/DNM1L Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00556-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026