Product Info Summary
| SKU: | PA1723 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-ABCB6 Antibody Picoband®
SKU/Catalog Number
PA1723
BA2764 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCB6 Antibody catalog # PA1723. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCB6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1723)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB6.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1723 is reactive to ABCB6 in Human
Observed Molecular Weight
94 kDa
Calculated molecular weight
93.9 kDa
Background of ABCB6
ABCB6, ATP-binding cassette sub-family B member 6, mitochondrialis a protein that in humans is encoded by the ABCB6 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This half-transporter likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1723 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, MCF-7 Whole Cell, A549 Whole Cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-ABCB6 antibody, PA1723, Western blotting
All lanes: Anti ABCB6 (PA1723) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 94KD
Observed bind size: 94KD
Click image to see more details
Anti-ABCB6 antibody, PA1723, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Specific Publications For Anti-ABCB6 Antibody Picoband® (PA1723)
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Customer Q&As
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7 Customer Q&As for Anti-ABCB6 Antibody Picoband®
Question
We are currently using anti-ABCB6 antibody PA1723 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-ABCB6 antibody (PA1723) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ABCB6 antibody PA1723. Let me know if you need anything else.
K. Johnson
Verified customer
Asked: 2020-04-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-20
Question
I see that the anti-ABCB6 antibody PA1723 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-27
Question
Please see the WB image, lot number and protocol we used for liver using anti-ABCB6 antibody PA1723. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-ABCB6 antibody (PA1723)?
H. Johnson
Verified customer
Asked: 2019-08-27
Answer
We do provide the blocking peptide for product anti-ABCB6 antibody (PA1723). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-27
Question
Will anti-ABCB6 antibody PA1723 work for ICC with liver?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
According to the expression profile of liver, ABCB6 is highly expressed in liver. So, it is likely that anti-ABCB6 antibody PA1723 will work for ICC with liver.
Boster Scientific Support
Answered: 2018-12-18
Question
Will anti-ABCB6 antibody PA1723 work on feline ICC with left ovary?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
Our lab technicians have not tested anti-ABCB6 antibody PA1723 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ABCB6 antibody PA1723 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline left ovary in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-19


