SKU PA1723
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, ICC, WB

Overview

Product Name Anti-ABCB6 Antibody
SKU/Catalog Number PA1723
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family B member 6, mitochondrial(ABCB6) detection. Tested with WB, IHC-P, ICC in Human.
Cite This Product Anti-ABCB6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1723)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB6(818-842aa YADMWQLQQGQEETSEDTKPQTMER).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Images And Assay Conditions

Anti-ABCB6 antibody, PA1723, Western blotting
All lanes: Anti ABCB6 (PA1723) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 94KD
Observed bind size: 94KD

Anti-ABCB6 antibody, PA1723, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q9NP58
Gene Name ABCB6
Protein Name ATP-binding cassette sub-family B member 6, mitochondrial
Tissue Specificity Widely expressed. High expression is detected in the retinal epithelium. .
Alternative Names ATP-binding cassette sub-family B member 6, mitochondrial;Mitochondrial ABC transporter 3;Mt-ABC transporter 3;P-glycoprotein-related protein;Ubiquitously-expressed mammalian ABC half transporter;ABCB6;MTABC3, PRP, UMAT;
Subcellular Localization Cell membrane. Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum. Golgi apparatus. Endosome . localized to the endosome- like compartement and dendrite tips.
Molecular Weight 93886 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Binds heme and porphyrins and functions in their ATP- dependent uptake into the mitochondria. Plays a crucial role in heme synthesis. .
Research Areas Human

*You can search these to find other products in these research areas.
Background ABCB6, ATP-binding cassette sub-family B member 6, mitochondrialis a protein that in humans is encoded by the ABCB6 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies(ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This half-transporter likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Polyclonal antibody for ABCB6 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ABCB6 information: Molecular Weight: 93886 MW; Subcellular Localization: Cell membrane. Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum. Golgi apparatus. Endosome . localized to the endosome- like compartement and dendrite tips; Tissue Specificity: Widely expressed. High expression is detected in the retinal epithelium.
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
PA1723
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting_box_image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
Write a review for PA1723
Copyright © 2019 Boster Biological Technology Inc. All rights reserved.