Product Info Summary
SKU: | PB9783 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Prion protein PrP/PRNP Antibody Picoband™
View all Prion protein Antibodies
SKU/Catalog Number
PB9783
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prion protein PrP/PRNP Antibody Picoband™ catalog # PB9783. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prion protein PrP/PRNP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9783)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRNP recombinant protein (Position: S143-S230).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9783 is reactive to PRNP in Human, Mouse, Rat
Applications
PB9783 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
30 kDa
Calculated molecular weight
8.691kDa
Background of Prion protein
PRNP (prion protein), also known as CD230 or PRP, is a protein that in humans is encoded by the PRNP gene. The major prion protein is expressed in the brain and several other tissues. Expression is most predominant in the nervous system but occurs in many other tissues throughout the body. Puckett et al. (1991) identified a RFLP with a high degree of heterozygosity in the 5-prime region of the PRNP gene, which might serve as a useful marker for the pter-p12 region of chromosome 20. PRNP is associated with a variety of cognitive deficiencies and neurodegenerative diseases such as Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, and kuru. PRNP is highly conserved through mammals, lending credence to application of conclusions from test animals such as mice. Comparison between primates is especially similar, ranging from 92.9-99.6% similarity in amino acid sequences.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PRNP using anti-PRNP antibody (PB9783).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRNP antigen affinity purified polyclonal antibody (Catalog # PB9783) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRNP at approximately 30 kDa. The expected band size for PRNP is at 30 kDa.
Click image to see more details
Figure 2. IHC analysis of PRNP using anti-PRNP antibody (PB9783).
PRNP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRNP Antibody (PB9783) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of PRNP using anti-PRNP antibody (PB9783).
PRNP was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRNP Antibody (PB9783) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PRNP (Source: Uniprot.org, NCBI)
Gene Name
PRNP
Full Name
Alternative prion protein
Weight
8.691kDa
Alternative Names
CD230; CJD; fatal familial insomnia); GSS; prion protein (p27-30); prion protein PrP; prion protein; prion-related protein; PRIPMGC26679 PRNP ASCR, AltPrP, CD230, CJD, GSS, KURU, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, p27-30 prion protein major prion protein|alternative prion protein|CD230 antigen|prion-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRNP, check out the PRNP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRNP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prion protein PrP/PRNP Antibody Picoband™ (PB9783)
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No publications found for PB9783
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Prion protein PrP/PRNP Antibody Picoband™
Question
you antibody using your anti-Prion protein PrP/PRNP antibody for ncam1 interactions studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
J. Mangal
Verified customer
Asked: 2020-02-11
Answer
We appreciate your inquiry. This PB9783 anti-Prion protein PrP/PRNP antibody is tested on rat pancreas tissue, brain tissue, tissue lysate, mouse pancreas tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-11
Question
Does anti-Prion protein PrP/PRNP antibody PB9783 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
According to the expression profile of brain, PRNP is highly expressed in brain. So, it is likely that anti-Prion protein PrP/PRNP antibody PB9783 will work for WB with brain.
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-Prion protein PrP/PRNP antibody PB9783 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
T. Banerjee
Verified customer
Asked: 2020-01-24
Answer
The anti-Prion protein PrP/PRNP antibody (PB9783) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Prion protein PrP/PRNP antibody PB9783. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
Do you have a BSA free version of anti-Prion protein PrP/PRNP antibody PB9783 available?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Prion protein PrP/PRNP antibody PB9783 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-23
Question
My question regards to test anti-Prion protein PrP/PRNP antibody PB9783 on mouse brain for research purposes, then I may be interested in using anti-Prion protein PrP/PRNP antibody PB9783 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The products we sell, including anti-Prion protein PrP/PRNP antibody PB9783, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-07
Question
I was wanting to use your anti-Prion protein PrP/PRNP antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
As indicated on the product datasheet, PB9783 anti-Prion protein PrP/PRNP antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-10
Question
Our lab were happy with the WB result of your anti-Prion protein PrP/PRNP antibody. However we have observed positive staining in brodmann (1909) area 23 cell membrane using this antibody. Is that expected? Could you tell me where is PRNP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
Based on literature, brodmann (1909) area 23 does express PRNP. Generally PRNP expresses in cell membrane. Regarding which tissues have PRNP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1363802, 1678248
Brain, and Ovary, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Prostate, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2019-04-05
Question
Is this PB9783 anti-Prion protein PrP/PRNP antibody reactive to the isotypes of PRNP?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
The immunogen of PB9783 anti-Prion protein PrP/PRNP antibody is E.coli-derived human PRNP recombinant protein (Position: S143-S230). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-31
Question
We purchased anti-Prion protein PrP/PRNP antibody for IHC on brain in the past. I am using rat, and We are going to use the antibody for WB next. Our lab want to know about examining brain as well as brain ovary in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
I took a look at the website and datasheets of our anti-Prion protein PrP/PRNP antibody and it appears that PB9783 has been validated on rat in both IHC and WB. Thus PB9783 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-12
Question
I see that the anti-Prion protein PrP/PRNP antibody PB9783 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-03
Question
Please see the WB image, lot number and protocol we used for brain using anti-Prion protein PrP/PRNP antibody PB9783. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-25
Question
Is a blocking peptide available for product anti-Prion protein PrP/PRNP antibody (PB9783)?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
We do provide the blocking peptide for product anti-Prion protein PrP/PRNP antibody (PB9783). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-23
Question
Would PB9783 anti-Prion protein PrP/PRNP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
As indicated on the product datasheet, PB9783 anti-Prion protein PrP/PRNP antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-08
Question
I have a question about product PB9783, anti-Prion protein PrP/PRNP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Huang
Verified customer
Asked: 2015-12-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9783 anti-Prion protein PrP/PRNP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-12-23
Question
We have observed staining in human brain ovary. Any tips? Is anti-Prion protein PrP/PRNP antibody supposed to stain brain ovary positively?
N. Collins
Verified customer
Asked: 2013-08-09
Answer
According to literature brain ovary does express PRNP. According to Uniprot.org, PRNP is expressed in brodmann (1909) area 23, brain, blood, prostate, brain ovary, leukemic t-cell, among other tissues. Regarding which tissues have PRNP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1363802, 1678248
Brain, and Ovary, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Prostate, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2013-08-09