Anti-TNF alpha Antibody Picoband™

TNF-alpha antibody

Boster Bio Anti-TNF alpha Antibody Picoband™ catalog # A00002-3. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 36 publication(s).

Product Info Summary

SKU: A00002-3
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-TNF alpha Antibody Picoband™

View all TNF-alpha Antibodies

SKU/Catalog Number

A00002-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TNF alpha Antibody Picoband™ catalog # A00002-3. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNF alpha Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human TNF alpha recombinant protein (Position: V77-L233).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00002-3 is reactive to TNF in Human

Applications

A00002-3 is guaranteed for ELISA, WB Boster Guarantee

Background of TNF-alpha

TNF (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For TNF (Source: Uniprot.Org, NCBI)

Uniprot ID

P01375

Gene ID

7124

Gene Name

TNF

Full Name

Tumor necrosis factor

Weight

25.644kDa

Superfamily

Tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNF, check out the TNF Infographic

TNF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00002-3 has been cited in 36 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of triptolide from Radix Tripterygium wilfordii (Leigongteng) on cartilage cytokines and transcription factor NF-%u03BAB: a study on induced arthritis in rats

Resveratrol attenuates hyperoxia%u2010induced oxidative stress, inflammation and fibrosis and suppresses Wnt/%u03B2%u2010catenin signalling in lungs of neonatal rats

Efficacy of acetylshikonin in preventing obesity and hepatic steatosis in db/db mice

Protective effects of baicalin on carbon tetrachloride induced liver injury by activating PPAR%u03B3 and inhibiting TGF%u03B21

Immunomodulatory properties of quercetin-3-O-%u03B1-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus

Neuroprotective effects of %u03B1-lipoic acid on long-term experimental autoimmune encephalomyelitis.

Polymeric micelles for potentiated antiulcer and anticancer activities of naringin

Rhein inhibits lipopolysaccharide-induced intestinal injury during sepsis by blocking the toll-like receptor 4 nuclear factor-%u03BAB pathway

Pannus inflammation in sacroiliitis following immune pathological injury and radiological structural damage: a study of 193 patients with spondyloarthritis

Involvement of tumor necrosis factor-%u03B1 in the upregulation of CXCR4 expression in gastric cancer induced by Helicobacter pylori

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model

Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats

CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment

Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication

Effect of Puerarin on Expression of ICAM-1 and TNF-? in Kidneys of Diabetic Rats

Hydrogen sulfide accelerates wound healing in diabetic rats

Molecular responses of radiation-induced liver damage in rats

Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation

Comparison of Serum Levels of Vitamin D and Inflammatory Markers Between Women With Gestational Diabetes Mellitus and Healthy Pregnant Control

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

In Vivo Evaluation of the Ameliorating Effects of Small-Volume Resuscitation with Four Different Fluids on Endotoxemia-Induced Kidney Injury

Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Gu R, Ding X, Tang W, Lei B, Jiang C, Xu G. Front Pharmacol. 2018 Apr 6;9:331. doi: 10.3389/fphar.2018.00331. eCollection 2018. A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal M

Liu Hp, Gao Zh, Cui Sx, Sun Df, Wang Y, Zhao Cr, Lou Hx, Qu Xj. Plos One. 2012;7(3):E33243. Doi: 10.1371/Journal.Pone.0033243. Epub 2012 Mar 14. Inhibition Of Intestinal Adenoma Formation In Apc(Min/+) Mice By Riccardin D, A Natural Product Derive...

Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.

Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.

Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.

Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.

Zhang Z, Wu Y, Zhao Y, Xiao X, Liu J, Zhou X. Exp Ther Med. 2013 May;5(5):1523-1527. Epub 2013 Mar 22. Dynamic Changes In Hmgb1 Levels Correlate With Inflammatory Responses During Cardiopulmonary Bypass.

Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.

Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.

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18 Customer Q&As for Anti-TNF alpha Antibody Picoband™

Question

Is this A00002-3 anti-TNF alpha antibody reactive to the isotypes of TNF?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

The immunogen of A00002-3 anti-TNF alpha antibody is E. coli-derived human TNF alpha recombinant protein (Position: V77-L233). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-12

Question

Our lab used your anti-TNF alpha antibody for WB on prostatic carcinoma in a previous experiment. I am using human, and We intend to use the antibody for ELISA next. I am interested in examining prostatic carcinoma as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

T. Parker

Verified customer

Asked: 2020-02-20

Answer

I looked at the website and datasheets of our anti-TNF alpha antibody and it seems that A00002-3 has been validated on human in both WB and ELISA. Thus A00002-3 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-20

Question

I see that the anti-TNF alpha antibody A00002-3 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-03

Question

Dear Support; What is the positive control slides I can use for this A00002-3 Anti-TNF alpha Antibody in Immunohistochemistry paraffin embedded sections? Best regards.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

For product A00002-3 Anti-TNF alpha Antibody, we recommend lung tissues. For your further references kindly read Tissue Antigens 16:30-48 (1980). PubMed: 6162228.

Boster Scientific Support

Answered: 2019-12-23

Question

I have an inquiry; what is the source of immunizing protein for this A00002-3 Anti-TNF alpha Antibody?, was the mouse immunized with native protein or recombinant expressed in E. coli, or insect cells or mammalian cells? Thank you,

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

We have the following information from the source : The A00002-3 Anti-TNF alpha Antibody, TNF-RII (p75/p80), clone MR2-1 were generated as described in the following article: Leeuwenberg, JFM et al; Slow release of soluble TNF-Receptors by monocytes in vitro. J Immunol 1994, 152: 4036. In this article you can see that sTNF-R75 was purified from culture supernatant of cells, transfected with the extracellular part of TNF-R75 by affinity chromatography on a TNF-coupled agarose column.

Boster Scientific Support

Answered: 2019-12-04

Question

Please see the WB image, lot number and protocol we used for blood using anti-TNF alpha antibody A00002-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-20

Question

I was wanting to use your anti-TNF alpha antibody for ELISA for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

It shows on the product datasheet, A00002-3 anti-TNF alpha antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-12

Question

Is a blocking peptide available for product anti-TNF alpha antibody (A00002-3)?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

We do provide the blocking peptide for product anti-TNF alpha antibody (A00002-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-06

Question

Hello Boster: I want to test A00002-3 Anti-TNF alpha Antibody in mouse samples. You think it is ok? Please let me know. Many thanks

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

This A00002-3 Anti-TNF alpha Antibody has been positively tested in Dog and Human species in our lab, but not tested in Mouse. Though, due to the high sequence homology between the protein

Boster Scientific Support

Answered: 2018-12-05

Question

We are currently using anti-TNF alpha antibody A00002-3 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

T. Edwards

Verified customer

Asked: 2018-11-28

Answer

The anti-TNF alpha antibody (A00002-3) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-28

Question

Can you help my question with product A00002-3, anti-TNF alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-11-05

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00002-3 anti-TNF alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-11-05

Question

Will A00002-3 anti-TNF alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Collins

Verified customer

Asked: 2018-08-16

Answer

It shows on the product datasheet, A00002-3 anti-TNF alpha antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-16

Question

My boss were satisfied with the WB result of your anti-TNF alpha antibody. However we have seen positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

Based on literature, leukocyte does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2017-11-30

Question

Would anti-TNF alpha antibody A00002-3 work for ELISA with blood?

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

According to the expression profile of blood, TNF is highly expressed in blood. So, it is likely that anti-TNF alpha antibody A00002-3 will work for ELISA with blood.

Boster Scientific Support

Answered: 2017-08-15

Question

you antibody to test anti-TNF alpha antibody A00002-3 on human blood for research purposes, then I may be interested in using anti-TNF alpha antibody A00002-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-22

Answer

The products we sell, including anti-TNF alpha antibody A00002-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-22

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-TNF alpha antibody A00002-3. Let me know if you need anything else.

J. Zhang

Verified customer

Asked: 2016-10-11

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-10-11

Question

We have observed staining in human blood. What should we do? Is anti-TNF alpha antibody supposed to stain blood positively?

Z. Collins

Verified customer

Asked: 2014-08-14

Answer

From what I have seen in literature blood does express TNF. From what I have seen in Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2014-08-14

Question

Do you have a BSA free version of anti-TNF alpha antibody A00002-3 available?

P. Wu

Verified customer

Asked: 2014-06-27

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TNF alpha antibody A00002-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-06-27

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00002-3

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A00002-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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