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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse LBP ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse LBP ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lbp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse LBP ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1274)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: G25-V481
1.56 ng/ml - 100 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1274 is reactive to Lbp in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1274 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1274-CAP||Anti-Mouse Lbp Pre-coated 96-well strip microplate||1|
|EK1274-ST||Mouse Lbp Standard||2 vials, 100 ng/tube|
|EK1274-DA||Mouse Lbp Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse LBP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lbp, Dilution ratio of 1:100, concentration in serum is around 500ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse LBP ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Lbp in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Lbp (Source: Uniprot.Org, NCBI)
LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233 Lbp|Bpif, Bpifd2, Ly88|lipopolysaccharide binding protein|lipopolysaccharide-binding protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Lbp, check out the Lbp Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Lbp: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1274 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xingwei Xiang,Xiaoling Zhou,Wenjie Wang,Yufang Zhou,Xuxia Zhou,Shanggui Deng,Bin Zheng,Zhengshun Wen,Effect of Antarctic Krill Phospholipid (KOPL) on High Fat Diet-Induced Obesity in Mice,Food Research International,2021,110456,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110456.
EK1274 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Dorigatti JD,Thyne KM,Ginsburg BC,Salmon AB.Beta-guanidinopropionic acid has age-specific effects on markers of health and function in mice.Geroscience.2021 Apr 23.doi:10.1007/s11357-021-00372-8.Epub ahead of print.PMID:33890206.
EK1274 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5
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