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Product Info Summary
| SKU: | A12898 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CPAMD8 Antibody Picoband®
SKU/Catalog Number
A12898
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CPAMD8 Antibody Picoband® catalog # A12898. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CPAMD8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12898)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CPAMD8 recombinant protein (Position: R58-D234).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12898 is reactive to CPAMD8 in Human, Monkey
Observed Molecular Weight
207 kDa
Calculated molecular weight
206.7 kDa
Background of CPAMD8
CPAMD8 belongs to the complement component-3 (C3; 120700)/alpha-2-macroglobulin (A2M; 103950) family of proteins, which are involved in innate immunity and damage control. The encoded protein is membrane-associated and proteolytically processed to generate two chains. Mutations in this gene cause a form of anterior segment dysgenesis, a developmental disorder of the eye. By genomic sequence analysis, the CPAMD8 gene is mapped to chromosome 19p13.3-p13.2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12898 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human placenta tissue, human T-47D whole cell, human U2OS whole cell, human K562 whole cell, human THP-1 whole cell, monkey COS-7 whole cell
IHC: human lung cancer tissue, human mammary cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPAMD8 antigen affinity purified polyclonal antibody (Catalog # A12898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPAMD8 at approximately 207KD. The expected band size for CPAMD8 is at 207KD.
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IHC analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).
CPAMD8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPAMD8 Antibody (A12898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).
CPAMD8 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPAMD8 Antibody (A12898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-CPAMD8 Antibody Picoband® (A12898)
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1 Customer Q&As for Anti-CPAMD8 Antibody Picoband®
Question
We are currently using anti-CPAMD8 antibody A12898 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
K. Bhatt
Verified customer
Asked: 2013-06-03
Answer
The anti-CPAMD8 antibody (A12898) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-03
