Product Info Summary
SKU: | M00002-2 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Pig, Primate |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IHC-F, ICC |
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Product info
Product Name
Anti-TNF alpha Purified Monoclonal Antibody
SKU/Catalog Number
M00002-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TNF alpha Purified Monoclonal Antibody (Catalog# M00002-2). Tested in Flow Cytometry, IHC-F, ICC, ELISA application(s). This antibody reacts with Pig, Human, Primate.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-TNF alpha Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00002-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MAb11
Isotype
Mouse IgG1 kappa
Immunogen
Recombinant human TNF-alpha. The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00002-2 is reactive to TNF in Human, Pig, Primate
Applications
M00002-2 is guaranteed for ELISA, Flow Cytometry, IHC-F, ICC Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
25.644kDa
Background of TNF-alpha
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1.
Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Flow cytometry: Intracellular staining.
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For TNF (Source: Uniprot.org, NCBI)
Gene Name
TNF
Full Name
Tumor necrosis factor
Weight
25.644kDa
Superfamily
Tumor necrosis factor family
Alternative Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNF, check out the TNF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF alpha Purified Monoclonal Antibody (M00002-2)
Hello CJ!
M00002-2 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dan min Wang,Yao Gong,Zhi duo Hou et al.Comparison of Sacroiliac Biopsies and Magnetic Resonance Imaging Examinations in Non-Radiographic Axial Spondyloarthritis, 05 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203
Species: Human
M00002-2 usage in article: APP:IHC, SAMPLE:SIJ TISSUE, DILUTION:NA
Dan min Wang,Yao Gong,Zhi duo Hou et al.Comparison of Sacroiliac Biopsies and Magnetic Resonance Imaging Examinations in Non-Radiographic Axial Spondyloarthritis, 05 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203
Species: Human
M00002-2 usage in article: APP:IHC, SAMPLE:SIJ TISSUE, DILUTION:NA
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