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Product Info Summary
| SKU: | M00002-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Pig, Primate |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IHC-F, ICC |
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Product info
Product Name
Anti-TNF alpha Purified Monoclonal Antibody
SKU/Catalog Number
M00002-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TNF alpha Purified Monoclonal Antibody (Catalog# M00002-2). Tested in Flow Cytometry, IHC-F, ICC, ELISA application(s). This antibody reacts with Pig, Human, Primate.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-TNF alpha Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00002-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MAb11
Isotype
Mouse IgG1 kappa
Immunogen
Recombinant human TNF-alpha. The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Reactive Species
M00002-2 is reactive to TNF in Human, Pig, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
25.6 kDa
Background of TNF
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00002-2 is guaranteed for ELISA, Flow Cytometry, IHC-F, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1.
Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Flow cytometry: Intracellular staining.
Validation Images & Assay Conditions
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ELISA analysis of TNF alpha (1 μg/ml) using mouse monoclonal antibody MAb11 (0.01 - 10 μg/ml).
Specific Publications For Anti-TNF alpha Purified Monoclonal Antibody (M00002-2)
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