Anti-TNF alpha Antibody Picoband®

Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-5).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant rat TNF alpha protein 5 ng,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (A00002-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17 kDa.

Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-5).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant mouse TNF alpha protein 5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (A00002-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17 kDa.

The role of gastric cancer cells in transforming macrophages in the TME. a The expression of M1 macrophage marker proteins in different groups. b The expression of M2 macrophage marker proteins in different groups. c Differential gene expression between M0 and gastric cancer cell metabolite intervention group. d Differential gene expression between M2 and gastric cancer cell metabolite intervention group. e Intersection statistics of differentially expressed genes between M2 and gastric cancer cell metabolite intervention group. f Intersection gene enrichment statistics. g RT-qPCR was used to detect the expression of CD86 mRNA. h RT-qPCR was used to detect the expression of iNOS mRNA. i RT-qPCR was used to detect the expression of CD206 mRNA. j RT-qPCR was used to detect the expression of IL1R2 mRNA. k RT-qPCR was used to detect the expression of CD163 mRNA. l RT-qPCR was used to detect the expression of TGFβ mRNA. m ELISA was used to detect TNF-α levels. n ELISA detects IL-10 levels. o ELISA detects the content of TGFβ1. *p<0.05; **p<0.01; ***p<0.001.
Index in PubMed under a CC BY license. PMID: 39991579

The effect of M2 subtype macrophages on TGFβ1 related pathway and epithelial mesenchymal transition in gastric cancer cells. a Cell intervention pattern diagram. b RT-qPCR was used to detect the levels of TGFβ mRNA in different intervention groups. c ELISA was used to test the expression of TNF-α in different intervention groups. d ELISA test the expression of IL-10 in different intervention groups. e ELISA was used to test the expression of TGFβ1 in different intervention groups. f The expression results of TGFβ1 protein. g The WB results of different interventions on histone expression. h The expression results of p-Smad2 protein. i The expression results of Smad2 protein. j The expression results of p-Smad3 protein. k The expression results of Smad3 protein. l The expression results of E-cadherin protein. m Results of N-cadherin protein expression. n Results of Vimentin protein expression. o RT-qPCR was used to detect the content of TGFβ mRNA at different intervention times. p ELISA was used to test the expression of TNF-α at different intervention times. q ELISA test the expression of IL-10 at different intervention times. r ELISA was used to test the expression of TGFβ1 at different intervention times. s WB results of protein expression at different intervention times. t The expression results of E-cadherin protein. u The expression results of N-cadherin protein. v Results of Vimentin protein expression. w Fluorescence results of mitochondrial membrane potential at different intervention times. Scale bar=50 μm. *p<0.05, **p<0.01, ***p<0.001.
Index in PubMed under a CC BY license. PMID: 39991579

Different inducers promote polarization of different subtypes of macrophages. a The CCK-8 method was used to detect the survival of M0 macrophages after 24 h of IL-4 intervention. b The CCK-8 method was used to detect the survival of M0 macrophages after 24 h of intervention with TGFβ1. c The CCK-8 method was used to detect the survival of M0 macrophages after LPS intervention for 24 h. d RT-qPCR was used to detect the expression of CD86 mRNA. e RT-qPCR was used to detect the expression of iNOS mRNA. f RT-qPCR was used to detect the expression of CD206 mRNA. g RT-qPCR was used to detect the expression of IL1R2 mRNA. h RT-qPCR was used to detect the expression of CD163 mRNA. i RT-qPCR was used to detect the expression of TGFβ mRNA. j ELISA detects TNF - α levels. k ELISA was used to detect IL-10 levels. l ELISA detects the content of TGFβ1. m The WB results of different interventions on histone expression. f TNF-α protein expression results. f IL-10 protein expression results. f TGFβ1 protein expression results. *p<0.05, **p<0.01, ***p<0.001.
Index in PubMed under a CC BY license. PMID: 39991579