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Tnf antibody (A00002-5) performs well in ELISA, WB and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 39 Publications

a00002 5 tnf primary antibodies wb testing 1_1

Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-5).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant rat TNF alpha protein 5 ng,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (A00002-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17 kDa.

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Anti-TNF alpha Antibody Picoband®

Tnf antibody

39 Publications

Reactive Species	Host	Application
Mouse, Rat	Rabbit	ELISA, WB

Tested Applications

Tnf antibody was positively detected by WB in:

recombinant mouse TNF alpha protein, recombinant rat TNF alpha protein

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ELISA

Mouse

Recommend

Rat

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Product Information

Product Name

Anti-TNF alpha Antibody Picoband®
View all TNF Antibodies

Form

Lyophilized

Uniprot

P06804

Gene ID

21926

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse TNF alpha/Tnf recombinant protein (Position: D89-E185).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

17 kDa

Calculated Molecular Weight

25.9 kDa

Background of Tnf

TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Cite This Product

Anti-TNF alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002-5)

Protocols

Western Blot protocol for Tnf antibody

Review Protocol

Publications

Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens

Published: 2026-01-23

Journal: POULTRY SCIENCE

Application: WB

Impact Factor: 4.20

Species: Mouse

PMID: 41616534

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Free Secondary Antibody

Anti-TNF alpha Antibody Picoband®

Tnf antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens

Bacillus amyloliquefaciens Fmb50 Alleviates Hyperglycemia by Reshaping Gut Structures and Mitigating Metabolic Disorders in Type 2 Diabetic Mice

Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells

Molecular mechanisms of dragon’s blood in treating ulcerative colitis based on NF-κb/NLPR3/Caspase-1 pyroptosis signaling pathway

Programmed Drug Delivering Janus Hydrogel Adapted to the Spatio-temporal Therapeutic Window for Achilles Tendon Repair

The mutualistic relationship between M2c macrophages of TGFβ1 induction and gastric cancer cells: the correlation between protective mechanisms in the tumor microenvironment and polarization of subtypes of cells

Injectable and self-healable hydrogel based on pullulan polysaccharide loading platelet-rich plasma and metal-phenol network nanoparticles for infectious wound healing

Reduction of TRPV1 expression on neurons due to downregulation of P2X7R in neonatal rat dorsal root ganglion satellite glial cells under co-culture conditions

Melatonin prevents bone loss in osteoporotic rats with valproic acid treatment by anti-inflammatory and anti-oxidative stress

Using broadly targeted plant metabolomics technology combined with network pharmacology to explore the mechanism of action of the Yishen Gushu formula in the treatment of postmenopausal osteoporosis in vivo

Anthrahydroquinone‑2,6‑disulfonate attenuates PQ‑induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of the PI3K/AKT/eNOS pathway

Oriented Fibers Cooperate with DFO to Prevent Tendon Adhesions by Improving the Repair Microenvironment

Taraxacum officinale-derived exosome-like nanovesicles modulate gut metabolites to prevent intermittent hypoxia-induced hypertension

Treatment of ulcerative colitis with Wu-Mei-Wan by inhibiting intestinal inflammatory response and repairing damaged intestinal mucosa

MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis

Key Role of Pro-inflammatory Cytokines in the Toxic Effect of Fluoride on Hepa1-6 Cells

Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats

Gypsophila elegans isoorientin-2″-O-α-l-arabinopyranosyl ameliorates porcine serum-induced immune liver fibrosis by inhibiting NF-κB signaling pathway and suppressing HSC activation

Puerarin exerts the protective effect against chemical induced dysmetabolism in rats

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Cerebralcare Granule®, a Chinese Herb Compound Preparation, Attenuates d-Galactose Induced Memory Impairment in Mice

Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats

Hydrogen sulfide accelerates wound healing in diabetic rats

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury

Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury

Protective effect of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats induced by yeast and potassium oxonate

Relationship between expression of COX-2, TNF-α, IL-6 and autoimmune-type recurrent miscarriage

Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats

Long-term resveratrol treatment prevents ovariectomy-induced osteopenia in rats without hyperplastic effects on the uterus

Alteration in P-glycoprotein at the blood–brain barrier in the early period of MCAO in rats

Protective roles of quercetin in acute myocardial ischemia and reperfusion injury in rats

Protective effects of propofol on endotoxemia-induced acute kidney injury in rats

Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats

Effects of triptolide from Radix Tripterygium wilfordii (Leigongteng) on cartilage cytokines and transcription factor NF-κB: a study on induced arthritis in rats

IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation

The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFα and IL-1β after cerebral ischemia in rats

Crocetin attenuates palmitate-induced insulin insensitivity and disordered tumor necrosis factor-α and adiponectin expression in rat adipocytes

Effect of Huwentoxin-I on the Fas and TNF apoptosis pathway in the hippocampus of rat with global cerebral ischemia

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