Anti-TNF alpha/Tnf Antibody Picoband™

TNF-alpha antibody

Boster Bio Anti-TNF alpha/Tnf Antibody Picoband™ catalog # A00002-5. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. Cited in 31 publication(s).

Product Info Summary

SKU: A00002-5
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, ICC, WB

Product Name

Anti-TNF alpha/Tnf Antibody Picoband™

View all TNF-alpha Antibodies

SKU/Catalog Number







Boster Bio Anti-TNF alpha/Tnf Antibody Picoband™ catalog # A00002-5. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNF alpha/Tnf Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002-5)




Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.




E.coli-derived mouse TNF alpha/Tnf recombinant protein (Position: D89-E185).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00002-5 is reactive to Tnf in Mouse, Rat


A00002-5 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Background of TNF-alpha

TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Flow Cytometry, 1-3μg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Tnf (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Tumor necrosis factor




tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha Tnf|DI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tna, Tnfs, Tnfsf1a, Tnlg1f, Tnf|tumor necrosis factor|tumor necrosis factor|cachectin|tumor necrosis factor ligand 1f|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnf, check out the Tnf Infographic

Tnf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00002-5 has been cited in 31 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Hydrogen sulfide accelerates wound healing in diabetic rats

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats

Effects of triptolide from Radix Tripterygium wilfordii ( Leigongteng ) on cartilage cytokines and transcription factor NF-κB: a study on induced arthritis in rats

Crocetin attenuates palmitate‐induced insulin insensitivity and disordered tumor necrosis factor‐α and adiponectin expression in rat adipocytes

Relationship between expression of COX-2, TNF-α, IL-6 and autoimmune-type recurrent miscarriage

Effect of Huwentoxin-I on the Fas and TNF apoptosis pathway in the hippocampus of rat with global cerebral ischemia

Gypsophila elegans isoorientin-2″-O-α-l-arabinopyranosyl ameliorates porcine serum-induced immune liver fibrosis by inhibiting NF-κB signaling pathway and suppressing HSC activation

Puerarin exerts the protective effect against chemical induced dysmetabolism in rats

The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFα and IL-1β after cerebral ischemia in rats

Cerebralcare Granule ® , a Chinese Herb Compound Preparation, Attenuates d -Galactose Induced Memory Impairment in Mice

IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation

Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats

Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats

Microstructural changes and immunohistological analysis of pro-inflammatory cytokines in spleens of lipopolysaccharide-induced rats.Indian Journal Of Animal Research.2019.(53):239-244
Species: Rat

Li HN,Yang QQ,Wang WT,Tian X,Feng F,Zhang ST,Xia YT,Wang JX,Zou YW,Wang JY,Zeng XY.Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.J Neuroinflammation.2021 Jul 5;18(1):150.doi:10.1186/s12974-021-02198-9.PMID:34225736;PMCID:PMC8258957.
Species: Rat
A00002-5 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION:1:500

Zhou Y,Zhao B,Zhang XL,Lu YJ,Lu ST,Cheng J,Fu Y,Lin L,Zhang NY,Li PX,Zhang J,Zhang J.Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration.Stem Cell Res Ther.2021 May 1;12(1):257.doi:10.1186/s13287-021-02287-9.PMID:33933157;PMCID:PMC8088044.
Species: Human,Mouse
A00002-5 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:200

Wang B,Gao C,Zhang P,Sun W,Zhang J,Gao J.The increased motion of lumbar induces ligamentum flavum hypertrophy in a rat model. BMC Musculoskelet Disord.2021 Apr 6;22(1):334.doi:10.1186/s12891-021-04203-x.PMID:33823825.
Species: Human,Rat
A00002-5 usage in article: APP:IHC, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:100

Yang Ping,Yingpeng Li,Shaowa Lü,Yali Sun,Wanmeng Zhang,Jialin Wu,Ting Liu,Yongji Li,A study of nanometre aggregates formation mechanism and antipyretic effect in Bai-Hu-Tang, an ancient Chinese herbal decoction,Biomedicine & Pharmacotherapy,Volume 124,202
Species: Rat

El Naggar EE,Mohamed EA,Borg TM,El-Sheakh AR,Hamed MF.Colon Targeting of Naringin for Enhanced Cytoprotection Against Indomethacin-Induced Colitis in Rabbits.Drug Des Devel Ther.2020 Feb 19;14:677-696.doi:10.2147/DDDT.S218357.PMID:32109993;PMCID:PMC703841
Species: New Zealand Rabbit
A00002-5 usage in article: APP:IHC, SAMPLE:COLON TISSUE, DILUTION:1:100

Baojian Wang,Chunyu Gao,Liguo Zhu et al.Lumbar Instability Induces Ligamentum Flavum Hypertrophy in a Rat Model, 08 January 2021, PREPRINT (Version 1) available at Research Square []
Species: Human,Rat

Yang QQ,Li HN,Zhang ST,Yu YL,Wei W,Zhang X,Wang JY,Zeng XY.Red nucleus IL-6 mediates the maintenance of neuropathic pain by inducing the productions of TNF-α and IL-1β through the JAK2/STAT3 and ERK signaling pathways.Neuropathology.2020 Aug;40(4):347-357
Species: Rat
A00002-5 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION:1:300

Lan Q,Lu R,Chen H,Pang Y, Xiong F,Shen C,Qin Z,Zheng L,Xu G,Zhao J.MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis.J Nanobiotechnology.2020 Aug 27;18(1):117.doi:10.1186/s12951-020-00666-7. PMID:32854712;PMCID:PMC7450974.
Species: Mouse
A00002-5 usage in article: APP:IF, SAMPLE: CHONDROCYTES, DILUTION:1:100

Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
A00002-5 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA

Guo Y,Gu R,Yu J,Lei B,Gan D,Xu G.Synthetic Glucocorticoid-Induced Leucine Zipper Peptide Inhibits Lipopolysaccharide-Induced Ocular Inflammation in Rats.Ophthalmic Res.2020;63(4):434-442.doi:10.1159/000505 003.Epub 2019 Nov 26.PMID:31770752.
Species: Rat
A00002-5 usage in article: APP:WB, SAMPLE: RETINAL TISSUE, DILUTION:1:500

Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
A00002-5 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000

Zhao B,Li S,Guo Z,Chen Z,Zhang X,Xu C,Chen J,Wei C. Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF-β1/Smads and NFκB pathways. Clin Exp Pharmacol Physiol.2020 Nov 11.doi:10.1111/1440-1681.13437.Ep
Species: Rat

Huang CL,Liu F,Zhang YY,Lin J,Fu M,Li YL, Zhou C,Li CJ,Shen JF. Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury. J Neurophysiol.2020 Dec 16.doi:10.1152/jn.00646. 202
Species: Rat
A00002-5 usage in article: APP:WB, SAMPLE:TG CELL AND SPVC CELL, DILUTION:1:1000

Tan Wf, Cao Xz, Wang Jk, Lv Hw, Wu By, Ma H. Clin Exp Pharmacol Physiol. 2010 Oct;37(10):1010-5. Doi: 10.1111/J.1440-1681.2010.05433.X. Protective Effects Of Lithium Treatment For Spatial Memory Deficits Induced By Tau Hyperphosphorylation In Sple...

Hou Sx, Zhu Wj, Pang Mq, Jeffry J, Zhou Ll. Food Chem Toxicol. 2014 Feb;64:57-64. Doi: 10.1016/J.Fct.2013.11.022. Epub 2013 Nov 26. Protective Effect Of Iridoid Glycosides From Paederia Scandens (Lour.) Merrill (Rubiaceae) On Uric Acid Nephropathy...

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