Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™

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The EK0411 Mouse IL-6 ELISA Kit have been tested in liver tissue lysate but we regret to say that we do not have any related information on brain lysate. But if you want to test this EK0411 Mouse IL-6 ELISA Kit on brain tissue lysate we strongly recommend diluting the samples at least 5-fold with assay diluent to minimized any effects of the lysis buffer and the samples may need to be diluted further but you have to determined it.

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A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-6 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

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A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

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A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

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A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

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A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

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A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

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A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

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The Mouse IL-6 ELISA Kit PicoKine™ (EK0411) has reactivity to mouse.

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For Mouse IL-6 ELISA Kit PicoKine EK0411, if your sample data points are within the middle of the curve, i.e. in the near linear region of the curve, then you can use the best fitting curve out of hyperbola, 4PL and 5PL. However, if your sample data points are near the ends of the standard curve, where it becomes not linear, it is recommended that you use 4PL for fitting, as it best fits the nature of the assay. It is also recommended that you adjust your assay so that the sample data points fall into the middle linear range of the curve. For further troubleshooting you may send us your generated data.

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Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.

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For the Mouse IL-6 ELISA Kit PicoKine™ (EK0411), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.