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Mouse IL-6 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-6. 96wells/kit, with removable strips. Cited in 167 publication(s). Independently reviewed in 2 review(s).
Reviews (2) See details
Mouse IL-6 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0411 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <1pg/ml |
| Assay Range: | 15.6pg/ml-1000pg/ml |
Introduction
SOX2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. SOX4 is important for early embryogenesis and for embryonic stem cell pluripotency.
Overview
| Product Name | Mouse IL-6 ELISA Kit PicoKine™
See all Il6 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0411 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse IL6 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL6 tissue specificity: |
| Cite This Product | Mouse IL-6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0411) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| Sensitivity | <1pg/ml |
| Assay Range | 15.6pg/ml-1000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: F25-T211 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | A2RTD1 |
|---|---|
| Gene Name | Il6 |
| Protein Name | Interleukin-6 |
| Subcellular Localization | Nucleus. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Mouse IL-6 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0411-CAP | 96-well plate precoated with anti-Mouse Il6 antibody | 1 |
| EK0411-ST | lyophilized recombinant Mouse Il6 standard | 10ng/tube |
| EK0411-DA | biotinylated anti-Mouse Il6 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0411 Mouse IL-6 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.032 | 0.085 | 0.141 | 0.237 | 0.452 | 0.816 | 1.470 | 2.281 |
We measured random samples of Mouse IL-6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 159 | 436 | 36 | 147 | 482 |
| Standard deviation | 2.41 | 7.79 | 34.94 | 2.91 | 7.93 | 40.48 |
| CV(%) | 6.7% | 4.9% | 7.2% | 8.1% | 5.4% | 8.4% |
We measured the performance consistency of Mouse IL-6 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 39 | 39 | 34 | 37 | 2.12 | 5.7% |
| Sample 2 | 159 | 147 | 154 | 170 | 157 | 8.38 | 5.3% |
| Sample 3 | 495 | 443 | 464 | 434 | 459 | 23.46 | 5.1% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 167
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| Inhibition of collar-induced carotid atherosclerosis by recombinant apoA-I cysteine mutants in apoE-deficient mice | PubMed ID 2975715 |
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| CD36 Participates in PrP106 | PubMed ID 22292032 |
| TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells | PubMed ID 25360740 |
| Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine | PubMed ID 25450719 |
| 3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis | PubMed ID 25874026 |
| Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase | PubMed ID 26191183 |
| Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells | PubMed ID 26295043 |
| Immunostimulative Activity of Low Molecular Weight Chitosans in RAW264.7 Macrophages | PubMed ID 26437419 |
| A deleterious role for Th9/IL-9 in hepatic fibrogenesis | PubMed ID 26728971 |
| Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition | PubMed ID 26793111 |
| Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-?-catenin axis | PubMed ID 26910375 |
| Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway | PubMed ID 26990441 |
| Klotho Contributes to Pravastatin Effect on Suppressing IL-6 Production in Endothelial Cells | PubMed ID 27034587 |
| PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice | PubMed ID 27054331 |
| Protective Effect of an Antibody against Specific Extracellular Domain of TLR2 on Agonists-Driven Inflammatory and Allergic Response | PubMed ID 27213155 |
| Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera | PubMed ID 27255791 |
| 4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner | PubMed ID 27276712 |
| MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3 | PubMed ID 27285980 |
| Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1? signaling pathway | PubMed ID 27398311 |
| Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration | PubMed ID 27411313 |
| Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity | PubMed ID 27463015 |
| Surfactin induces maturation of dendritic cells in vitro | PubMed ID 27534429 |
| Mature dendritic?cells?cause Th17/Treg imbalance by secreting TGF-?1 and IL-6 in the pathogenesis of experimental autoimmune encephalomyelitis | PubMed ID 27536199 |
| Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination | PubMed ID 27586056 |
| LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling | PubMed ID 27590510 |
| MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response | PubMed ID 27725852 |
| In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly | PubMed ID 27847405 |
| NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-?B Signaling Pathway | PubMed ID 27956761 |
| Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-?B Signaling Pathway | PubMed ID 28119924 |
| 5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice | PubMed ID 28138699 |
| Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice | PubMed ID 28178855 |
| H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375 | PubMed ID 28382020 |
| A novel pH?controlled hydrogen sulfide donor protects gastric mucosa from aspirin?induced injury | PubMed ID 28387464 |
| High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage | PubMed ID 28417961 |
| The upregulation of integrin ?D?2 (CD11d/CD18) on inflammatory macrophages promotes macrophage retention in vascular lesions and development of atherosclerosis | PubMed ID 28500072 |
| Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1 | PubMed ID 28586010 |
| Involvement of receptor-interacting protein 140 in palmitate-stimulated macrophage infiltration of pancreatic beta cells | PubMed ID 28672957 |
| Evaluation of the chemical composition, antioxidant and anti-inflammatory activities of distillate and residue fractions of sweet basil essential oil | PubMed ID 28720944 |
| Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice | PubMed ID 28977826 |
| Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial | PubMed ID 29887900 |
| 3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation | PubMed ID 29887940 |
| Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway?in vitro | PubMed ID 26617802 |
| Ascorbic acid ameliorates oxidative stress and inflammation in dextran sulfate sodium-induced ulcerative colitis in mice. | PubMed ID 26884937 |
| Nobiletin attenuates lipopolysaccharide/D%u2011galactosamine%u2011induced liver injury in mice by activating the Nrf2 antioxidant pathway and subsequently inhibiting NF%u2011%u03BAB %u2026 | PubMed ID 27878238 |
| Exclusive enteral nutrition protects against inflammatory bowel disease by inhibiting NF%u2011%u03BAB activation through regulation of the p38/MSK1 pathway | PubMed ID 29901086 |
| Whey Acidic Protein/Four-Disulfide Core Domain 21 Regulate Sepsis Pathogenesis in a Mouse Model and a Macrophage Cell Line via the Stat3/Toll-Like Receptor 4 %u2026 | PubMed ID 29900929 |
| Assessment of the involvement of the macrophage migration inhibitory factor%u2013glucocorticoid regulatory dyad in the expression of matrix metalloproteinase%u20102 during %u2026 | PubMed ID 28776753 |
| E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury | PubMed ID 28740545 |
| From the Cover: Lung-Specific Overexpression of Constitutively Active IKK2 Induces Pulmonary and Systemic Inflammations but Not Hypothalamic Inflammation and %u2026 | PubMed ID 29036520 |
| Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome | PubMed ID 29062341 |
| Protection of SAL B with H9C2 cells | PubMed ID 26705025 |
| Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis | PubMed ID 27152709 |
| Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury | PubMed ID 29188738 |
| Novel therapeutic approach by nicotine in experimental model of multiple sclerosis | PubMed ID 23696955 |
| The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells | PubMed ID 30010837 |
| Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026 | PubMed ID 25412187 |
| Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026 | PubMed ID 25412187 |
| Procyanidin B2 ameliorates carrageenan-induced chronic nonbacterial prostatitis in rats via anti-inflammatory and activation of the Nrf2 pathway | PubMed ID 28844677 |
| CFTR protects against vascular inflammation and atherogenesis in apolipoprotein E-deficient mice | PubMed ID 28615349 |
| Increased expression of CXCR5 and CXCL13 in mice with experimental autoimmune myocarditis | PubMed ID 28485792 |
| Novel Phosphodiesterase 4 Inhibitor FCPR03 Alleviates Lipopolysaccharide-Induced Neuroinflammation by Regulation of the cAMP/PKA/CREB Signaling %u2026 | PubMed ID 28450469 |
| Aryl Hydrocarbon Receptor Promotes IL-10 Expression in Inflammatory Macrophages Through Src-STAT3 Signaling Pathway | PubMed ID 30283437 |
| %u516D%u541B%u5B50%u6C64%u63D0%u53D6%u7269%u6291%u5236%u98DF%u7BA1%u764C Ec9706 %u7EC6%u80DE%u589E%u6B96%u53CA%u5176%u5BF9%u4FE1%u53F7%u8F6C%u5BFC%u548C%u8F6C%u5F55%u6D3B%u5316%u56E0%u5B50 3 %u4FE1%u53F7%u901A%u8DEF%u7684%u5F71%u54CD | PubMed ID 30362164 |
| Activation of the p38 MAPK/NF-%u03BAB pathway contributes to doxorubicin-induced inflammation and cytotoxicity in H9c2 cardiac cells | PubMed ID 23807148 |
| Induced pluripotent stem cells inhibit bleomycin-induced pulmonary fibrosis in mice through suppressing TGF-%u03B21/Smad-mediated epithelial to mesenchymal %u2026 | PubMed ID 27895584 |
| Apigenin Inhibits the Expression of IL-6, IL-8, and ICAM-1 in DEHP-Stimulated Human Umbilical Vein Endothelial Cells and In Vivo | PubMed ID 22527144 |
| Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL‐6/HIF‐1α signaling pathway | PubMed ID 27398311 |
| Magnolol abrogates chronic mild stress-induced depressive-like behaviors by inhibiting neuroinflammation and oxidative stress in the prefrontal cortex of mice. | PubMed ID 29627576 |
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2 reviews
"Great IL-6 Kit from Boster!"
Source: Biocompare.com
"Great IL-6 Kit from Boster!"
--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician
| Application | ELISA |
|---|---|
| Sample | Mouse serum |
| Detection | -- |
"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")
"IL-6 Estimation in Cell Culture Supernatant"
Source: Biocompare.com
"IL-6 Estimation in Cell Culture Supernatant"
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
| Application | ELISA |
|---|---|
| Sample | Cell culture media |
| Detection | -- |
"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")
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Questions and answers from customers related to EK0411 Mouse IL-6 ELISA Kit PicoKine™
1 Related Questions
Question
Q: What are some alternative names that could be used to describe this product?
Verified Customer
Asked: 0000-00-00
Answer
A: Some common names include but are not limited to mouse il 6 elisa kit, mouse il-6 elisa kit, mouse il6 elisa kit, mouse interleukin 6 elisa kit, mouse interleukin-6 elisa kit
Boster Scientific Support
Answered: 0000-00-00
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Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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