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Product Info Summary
| SKU: | EK0411 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
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Product info
Product Name
Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™
SKU/Catalog Number
EK0411
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0411)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: F25-T211
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0411 is reactive to Il6 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Application Guarantee
EK0411 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0411-CAP | Anti-Mouse Il6 Pre-coated 96-well strip microplate | 1 |
| EK0411-ST | Mouse Il6 Standard | 2 vials, 10 ng/tube |
| EK0411-DA | Mouse Il6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.032 | 0.085 | 0.141 | 0.237 | 0.452 | 0.716 | 1.270 | 1.981 |
Data Example Images
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Mouse IL-6 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-6, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of IL-6 in samples: 23001459 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 36 | 159 | 436 | 36 | 147 | 482 |
| Standard deviation | 2.41 | 7.79 | 34.94 | 2.91 | 7.93 | 40.48 |
| CV (%) | 6.7% | 4.9% | 7.2% | 8.1% | 5.4% | 8.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 39 | 39 | 34 | 37 | 2.12 | 5.7% |
| Sample 2 | 159 | 147 | 154 | 170 | 157 | 8.38 | 5.3% |
| Sample 3 | 495 | 443 | 464 | 434 | 459 | 23.46 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For Il6 (Source: Uniprot.Org, NCBI)
Uniprot ID
P08505
Gene ID
16193
Gene Name
Il6
Full Name
Interleukin-6
Weight
24.384kDa
Superfamily
IL-6 superfamily
Alternative Names
B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A Il6|Il, Il-6|interleukin 6|interleukin-6|B-cell hybridoma growth factor|interleukin HP-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il6, check out the Il6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™ (EK0411)
EK0411 has been cited in 228 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Native nanodiscs from blood inhibit pulmonary fibrosis
Species: Mouse
In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus
Species: Mouse
Engineered nanoparticles disguised as macrophages for trapping lipopolysaccharide and preventing endotoxemia
Species: Mouse
VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
Species: Mouse
Terazosin activates Pgk1 and Hsp90 to promote stress resistance
Species: Mouse
Clara cell 10-kDa protein inhibits TH17 responses through modulating dendritic cells in the setting of allergic rhinitis
Species: Mouse
Melatonin alleviates cadmium‐induced liver injury by inhibiting the TXNIP‐NLRP3 inflammasome
Species: Mouse
Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery
Species: Mouse
Astrocyte-derived ATP modulates depressive-like behaviors
Species: Mouse
Wu JY,Li YJ,Hu XB,Huang S,Luo S,Tang T,Xiang DX.Exosomes and biomimetic nanovesicles-mediated anti-glioblastoma therapy: A head-to-head comparison.J Control Release.2021 Jul 6;336:510-521.doi:10.1016/j.jconrel.2021.07.004.Epub ahead of print.PMID:34237399.
Species: Human,Mouse,Zebrafish
EK0411 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Wei,L.,Liu,Y.,Liu,Z.H.I.P.I.N.G.,Mao,Q.I.C.H.A.O.,Shi,N.,& Yang,J.I.A.N.F.E.I.(2021).Inhibitory Effects of Astragalus Polysaccharide on Myocardial Apoptosis Induced by Hypoxia or Reoxygenation in Rats.Indian Journal of Pharmaceutical Sciences,83(3),614-617.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:MYOCARDIAL CELL, DILUTION:NA
Yanyan Yu,Xiangqian Li,Wenpeng Hu,et al.Self-developed NF-κB inhibitor 270 protects against LPS-induced acute kidney injury and lung injury through improving inflammation.Authorea.June 22,2021.
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:PLASMA AND BMDMS, DILUTION:NA
Shicheng Xu,Anping Guo,Nan-nan Chen,Wei Dai,Huan-aoyu Yang,Wenqin Xie,Mengjie Wang,Qi-Dong You,Xiao-Li Xu,Design and synthesis of Grp94 selective inhibitors based on Phe199 induced fit mechanism and their anti-inflammatory effects,European Journal of Medicinal Chemistry,2021,113604,ISSN 0223-5234,https://doi.org/10.1016/j. ejmech.2021.113604.
Species: Mouse,Rat
EK0411 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
Zhang,J.,Zhu,L.,Shi,H.,& Zheng,H.(2021).Protective effects of miR‑155‑5p silencing on IFN‑γ‑induced apoptosis and inflammation in salivary gland epithelial cells.Experimental and Therapeutic Medicine,22,882.https://doi.org/10.3892/etm.2021.10314
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SGECS, DILUTION:NA
Qiu-yue Fang,Shu-ping Chen,Jun-qiao Wang,Xiao-jun Huang,Qi-xing Nie,Glyn O. Philips,Steve W. Cui,Ya-jing Li,Shao-ping Nie,Fractions from natural Cordyceps sinensis alleviated intestinal injury in Cyclophosphamide-induced mice,Bioactive Carbohydrates and Dietary Fibre,2021,100271,ISSN 2212-6198,https://doi.org/10.1016/j.bcdf.2021.100271.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SMALL INTESTINE, DILUTION:NA
Tawfiq,R.A.,Attia,Y.M.,Nassar,N.N.,Hammam,O.A.,Elmazar,M.M.,& Abdallah,D.M.(2021).Insights into the Impact of FXR Activation on Hepatic Autophagy in a Non-alcoholic Steatohepatitis Model.Bulletin of Pharmaceutical Sciences.Assiut,44(1),265-274.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA
Lv M,Xue G,Cheng H,Meng P,Lian X,Hölscher C,Li D. The GLP-1/GIP dual-receptor agonist DA5-CH inhibits the NF-κB inflammatory pathway in the MPTP mouse model of Parkinson‘s disease more effectively than the GLP-1 single-receptor agonist NLY01. Brain Behav. 2021 Jun 14. doi: 10.1002/brb3.2231. Epub ahead of print. PMID: 34125470.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Feng Z,Peng S,Wu Z,Jiao L,Xu S,Wu Y,Liu Z,Hu Y,Liu J,Wu Y,Wang D.Ramulus mori polysaccharide-loaded PLGA nanoparticles and their anti-inflammatory effects in vivo.Int J Biol Macromol.2021 Jun 1:S0141-8130(21)01184-3.doi:10.1016/j.ijbiomac.2021.05.200.Epub ahead of print.PMID:34087293.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:COLON, DILUTION:NA
WEI Wen-Long,WU Shi-Fei,LI Hao-Jv,LI Zhen-Wei,QU Hua,YAO Chang-Liang,ZHANG Jian-Qing,LI Jia-Yuan,WU Wan-Ying , GUO De-An.Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS [J].Chin J Nat Med,2021,19(6):473-480.doi:10.1016/S1875-5364(21)60046-8
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Zhang W,Li J,Yao H,Li T.Restoring microRNA-499-5p Protects Sepsis-Induced Lung Injury Mice Via Targeting Sox6.Nanoscale Res Lett.2021 May 21;16(1):89.doi:10.1186/s11671-021-03534-x.PMID:34019224.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Zheng,W.,Xie,Q.,Zhang,Z.et al.Inhibited HDAC3 or Elevated MicroRNA-494-3p Plays a Protective Role in Myocardial Ischemia-Reperfusion Injury via Suppression of BRD4.Mol Neurobiol (2021).https://doi.org/10.1007/s12035-021-02369-y
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION: NA
Li D,Weng Y,Wang G,Zhen G.Anti-Septic Potential of 7-α-Obacunyl Acetate Isolated from the Toona sinensis on Cecal Ligation/Puncture Mice via Suppression of JAK-STAT/NF-κB Signal Pathway.Infect Drug Resist.2021;14:1813-1821https://doi.org/10.2147/IDR.S302853
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Wang Y,Gao Y,Huang Y,Pan Y,Yu Y,Zhou Y,Wan SS,Yang YN.The potential protective effects of miR-497 on corneal neovascularization are mediated via macrophage through the IL-6/STAT3/VEGF signaling pathway.Int Immunopharmacol.2021 May 10;96:107745.doi:10.1016/j.intimp.2021. 107745. Epub ahead of print.PMID:33984719.
Species: Human,Mouse
EK0411 usage in article: APP: ELISA, SAMPLE:CORNEAL TISSUE, DILUTION:NA
Ai Z,Cheng C,Zhou L,Yin S,Wang L,Liu Y.Bone marrow mesenchymal stem cells-derived extracellular vesicles carrying microRNA-221-3p protect against ischemic stroke via ATF3.Brain Res Bull.2021 Apr 28:S0361-9230(21)00125-8.doi:10.1016/j.brainresbull.2021.04.022.Epub ahead of print.PMID:33932490.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BMSCS AND BRAIN TISSUE, DILUTION:NA
Yanyan Wang,Hui Zhang,Zhibin Wang et al.Anti-Psoriasis Inflammation And Anti-Oxidation of The Extract From The Flower of Datura Metel L.,16 April 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-396730/v1]
Species: Pig
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Cui H,Cheng Y,He Y,Cheng W,Zhao W,Zhao H,Zhou FH,Wang L,Dong J,Cai S.The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.Mol Med Rep.2020 Nov;22(5):3723-3734.doi:10.3892/mmr.2020.11450.Epub 2020 Aug 21.PMID:33000187;PMCID:PMC7533517.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Guo J,Li M,Yang Y,Zhang L,Zhang LW,Sun QY.Pretreatment with atorvastatin ameliorates cobra venom factor-induced acute lung inflammation in mice.BMC Pulm Med.2020 Oct 12;20(1):263.doi:10.1186/s12890-020-01307-3.PMID:33046059;PMCID:PMC7552367.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Fu,Y.;Leng,C.;Fan,Y.;Ma,X.;Li,X.;Wang,X.;Guo,Z.;Wang,X.;Shang,R.In Vitro and In vivo Activity of 14-O-[(4,6-Diamino-Pyrimidine-2-yl) Thioacetyl] Mutilin Against Methicillin-Resistant Staphylococcus aureus.Preprints 2021,2021040285
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Luo YY,Yang ZQ,Lin XF,Zhao FL,Tu HT,Wang LJ,Wen MY,Xian SX.Knockdown of lncRNA PVT1 attenuated macrophage M1 polarization and relieved sepsis induced myocardial injury via miR-29a/HMGB1 axis.Cytokine.2021 Apr 9:155509.doi:10.1016/j.cyto.2021.155509.Epub ahead of print.PMID:33840587.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BMDMS, DILUTION:NA
Zhang Y,Wang R,Shi W,Zhihui Z,Wang X,Li C,Zhang S,Zhang P. Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway.J Ethnopharmacol.2021 Apr 1:114063.doi:10. 1016/j.jep.2021.114063.Epub ahead of print.PMID:33813013.
Species: Mouse,Canine
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Shi Y,Qiao CM,Zhou Y,Wu J,Cui C,Hong H,Jia XB,Huang SB,Yao L,Zhao WJ,Shen YQ.Protective effects of prucalopride in MPTP-induced Parkinson‘s disease mice: Neurochemistry, motor function and gut barrier.Biochem Biophys Res Commun.2021 Apr 6;556:16-22.doi:10.1016/j. bbrc.2021.03.109.Epub ahead of print.PMID:33836343.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
Pingjun Zhu,Kun Wan,Ming Yin,Peng Hu,Yifan Que,Xin Zhou,Lei Zhang,Tianzhi Li,Yingzhen Du,Guogang Xu,Xiangqun Fang,"RIPK3 Induces Cardiomyocyte Necroptosis via Inhibition of AMPK-Parkin-Mitophagy in Cardiac Remodelling after Myocardial Infarction",Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 6635955,11 pages,2021.https://doi.org/10.1155/2021/6635955
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Yin S, Li L, Tao Y, Yu J, Wei S, Liu M and Li J (2021) The Inhibitory Effect of Artesunate on Excessive Endoplasmic Reticulum Stress Alleviates Experimental Colitis in Mice. Front. Pharmacol. 12:629798. doi: 10.3389/fphar.2021.629798
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
Xin Yi, Jie Liu, Mo-sha Cheng, Qing Zhou,Low-intensity pulsed ultrasound inhibits IL-6 in subchondral bone of temporomandibular joint osteoarthritis by suppressing the TGF-β1/Smad3 pathway,Archives of Oral Biology,2021,105110,ISSN 0003-9969,https://doi.org/10.1016/j.archoralbio.2021.105110.
Species: New Zealand White Rabbit,Mouse
Xiang XW,Zhou XL,Wang R,Shu CH,Zhou YF,Ying XG,Zheng B.Protective Effect of Tuna Bioactive Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice.Mar Drugs.2021 Feb 26;19(3):127.doi:10.3390/md19030127.PMID:33652919.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Jie Cheng, Min Chen, Hui-Qi Wan, Xue-Qin Chen, Cheng-Fu Li, Ji-Xiao Zhu, Qing Liu, Guang-Hui Xu, Li-Tao Yi,Paeoniflorin exerts antidepressant-like effects through enhancing neuronal FGF-2 by microglial inactivation,Journal of Ethnopharmacology,2021,114046,ISSN 0378-8741,https://doi.org/10.1016/j.jep.2021.114046.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:NA
Yang, Y., Deng, R., Chen, Z., Yao, L., Yang, X., & Xiang, D. (2021). Piperazine ferulate attenuates high glucose‑induced mesangial cell injury via the regulation of p66Shc. Molecular Medicine Reports, 23, 374. https://doi.org/10.3892/mmr.2021.12013
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SV-40 CELL AND MES-13 CELL, DILUTION:NA
Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Natarajan Bhaskaran,Sangeetha Jayaraman,Cheriese Quigley,Prerna Mamileti,Mahmoud Ghannoum,Aaron Weinberg,Jason Thuener,Quintin Pan,Pushpa Pandiyan bioRxiv 2021.02.15.431361;doi: https://doi.org/10.1101/2021.02.15.431361
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:TONGUE TISSUE, DILUTION:NA
Liu Y,Qi Y.Vildagliptin, a CD26/DPP4 inhibitor, ameliorates bleomycin-induced pulmonary fibrosis via regulating the extracellular matrix.Int Immunopharmacol.2020 Oct;87:106774.doi:10.1016/j.intimp.2020.106774.Epub 2020 Jul 27. PMID:32731178.
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:PLASMA AND LUNG TISSUE, DILUTION:NA
Zou XY,Xie RR,Li W,Su CL,Chen YS,Tang H.Novel sampangine derivatives as potent inhibitors of Cu2+-mediated amyloid-β protein aggregation, oxidative stress and inflammation.Int J Biol Macromol.2021 Jan 18:S0141-8130(21)00123-9.doi:10.1016/j.ijbiomac.2021.0
Species: Mouse,Worms
EK0411 usage in article: APP:ELISA, SAMPLE:BV2 CELL, DILUTION:NA
Sharma D,Arora S,Banerjee A,Singh J.Improved insulin sensitivity in obese-diabetic mice via chitosan Nanomicelles mediated silencing of pro-inflammatory Adipocytokines.Nanomedicine.2021 Jan 15:102357.doi:10.1016/j.nano.2020.102357.Epub ahead of print. PMI
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:ADIPOSE TISSUE, DILUTION:NA
Cao Y,Li Y,He C,Yan F,Li JR,Xu HZ,Zhuang JF,Zhou H,Peng YC,Fu XJ,Lu XY,Yao Y,Wei YY,Tong Y,Zhou YF,Wang L.Selective Ferroptosis Inhibitor Liproxstatin-1 Attenuates Neurological Deficits and Neuroinflammation After Subarachnoid Hemorrhage.Neurosci Bull.202
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Liu Y,Wang T,Si B,Du H,Liu Y,Waqas A,Huang S,Zhao G,Chen S,Xu A.Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota. EcotoxicolEnviron Saf.2021 Jan 8;210:111903.doi:10.1016/j.eco
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA
Li H,Bi Q,Cui H,Lv C,Wang M.Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma. BMC Complement Med Ther.2021 Jan 7;21(1):21.doi:10.1186/s12906-020-03187-w.PMID:33413331;PMCID:PMC7792286.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Li Y,Song W,Tong Y,Zhang X,Zhao J,Gao X,Yong J,Wang H.Isoliquiritin ameliorates depression by suppressing NLRP3-mediated pyroptosis via miRNA-27a/SYK/NF-κB axis.J Neuroinflammation.2021 Jan 5;18(1):1.doi:10.1186/s12974-020-02040-8.PMID:33402173;PMCID:PMC7
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM AND HIPPOCAMPUS TISSUE, DILUTION:NA
Chen X,He Y,Zhu Y,Du J,Sun H.linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses.Cell Rep.2021 Jan 5;34(1):108584.doi:10.1016/j.celrep.2020.108584.PMID:33406422.
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Wang W,Xu RL,He P,Chen R.MAR1 suppresses inflammatory response in LPS-induced RAW 264.7 macrophages and human primary peripheral blood mononuclear cells via the SIRT1/PGC-1α/PPAR-γ pathway.J Inflamm (Lond).2021 Feb 8;18(1):8.doi:10.1186/s12950-021-00271-x
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973. doi:10.1155/2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973.doi:10.1155/ 2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Dong-Wei Wang,Shu-Juan Li,Xue-Ying Tan,Jin-Hui Wang,Ying Hu,Zeng Tan,Jing Liang,Jing-Bo Hu,Yan-Guo Li,Yu-Fen Zhao,Engineering of stepwise-targeting chitosan oligosaccharide conjugate for the treatment of acute kidney injury,Carbohydrate Polymers,Volume 25
Species: Human,Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:RENAL TISSUE, DILUTION:NA
Xin Li,Licong Yang,Meng Xu,Gaoxiang Qiao,ChangJiang Li,Lezhen Lin,Guodong Zheng,Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice,Journal of Functional Foods,Volume
Species: Mouse
EK0411 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Long-Wei Li,Xiao-Yi Chen,Lan-Cong Liu,Yi Yang,You-Jiao Wu,Gang Chen,Zhi-Feng Zhang,Pei Luo,Oil-in-water camellia seeds oil nanoemulsions via high pressure microfluidization: Formation and evaluation,LWT,2020, 110815,ISSN 0023-6438,https://doi.org/10.1016/
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Tao Huang,Yurong Zhang,Wenjuan Zhang,Tingting Lin,Luoting Chen,Bei Yang,Lei Wu,Jianhua Yang,Dalei Zhang,"Attenuation of Perfluorooctane Sulfonate-Induced Steatohepatitis by Grape Seed Proanthocyanidin Extract in Mice",BioMed Research International,vol.202
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Mingyue Shen,Xianxiang Chen,Lixin Huang,Qiang Yu,Yi Chen,Jianhua Xie,Sulfated Mesona chinensis Benth polysaccharide enhance the immunomodulatory activities of cyclophosphamide-treated mice,Journal of Functional Foods,Volume 76,2021,104321,ISSN 1756-4646,h
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EK0411 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELLS, DILUTION:NA
Mu,D,Li,D,Li,J,et al. Long non‐coding RNA HULC protects against atherosclerosis via inhibition of PI3K/AKT signaling pathway. IUBMB Life.2020;1– 11.https://doi.org/10.1002/iub.2417
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Xinxin Zhang,Yating Qin,Xiaoning Wan et al. Rosuvastatin Inhibits Atherosclerosis Development by Improving Lipid Accumulation and Polarization Conversion of Macrophages via Regulating Autophagy, 19 November 2020,PREPRINT (Version 1) available at Research
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Gao Y,Zhou X,Zhou Y,Zhang W,Zhao L.Chrysene accelerates the proceeding of chronic obstructive pulmonary disease with the aggravation of inflammation and apoptosis in cigarette smoke exposed mice.Hum Exp Toxicol.2020 Dec 20:960327120979343.doi:10. 1177/096
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Fan J,Zhang YC,Zheng DF,Zhang M,Liu H,He M,Wu ZJ.IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model. Cytokine.2020 Mar;127:154936.doi: 10.1016/j.cyto.2019.154936.Epub 2019 Nov 28.PMID:317865
Species: Human, Mouse
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Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
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EK0411 usage in article: APP:ELISA, SAMPLE:CORTEX AND HIPPOCAMPUS TISSUE, DILUTION:NA
He L,Wang X,Cheng D,Xiong Z,Liu X. Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 in the uterus in premature ovarian insufficiency mouse models. Mol Med Rep.2021 Jan;23(1):37.doi:10.3892/mmr.2020.
Species: Mouse
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Zhao S, Fan Z, Hu J, Zhu Y, Lin C, Shen T, Li Z, Li K, Liu Z, Chen Y, Zhang B. The differential effects of isoflurane and sevoflurane on neonatal mice. Sci Rep. 2020 Nov 9;10(1):19345. doi: 10.1038/s41598-020-76147-6. PMID: 33168900; PMCID: PMC7652873.
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Zhang M, Gao J, Zhao X, Zhao M, Ma D, Zhang X, Tian D, Pan B, Yan X, Wu J, Meng X, Yin H, Zheng L. p38α in macrophages aggravates arterial endothelium injury by releasing IL-6 through phosphorylating megakaryocytic leukemia 1. Redox Biol. 2020 Nov 1;38:10
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Zhang Z,Jiang S,Tian H,Zeng Y,He K,Lin L,Yu F. Ethyl acetate fraction from Nymphaea hybrida Peck modulates inflammatory responses in LPS-stimulated RAW 264.7 cells and acute inflammation murine models. J Ethnopharmacol. 2020 Dec 15:113698.doi:10.1016/j.je
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Zheng B, Ying M, Xie J, Chen Y, Wang Y, Ding X, Hong J, Liao W, Yu Q. A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier. Food Funct
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Zhou Q, Guo D, Li X, Wang Y, Ye X, Xue S, Wang X. Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK. An Acad Bras Cienc. 2020 Nov 20;92(4):e20200241. doi: 10.1590/0001-3765202020200241. PMID: 33237143.
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Du Y, Liu G, Zhao L, Yao R. Protective Effect of miR-204 on Doxorubicin-Induced Cardiomyocyte Injury via HMGB1. Oxid Med Cell Longev. 2020 Nov 19;2020:8819771. doi: 10.1155/2020/8819771. PMID: 33274007; PMCID: PMC7695502.
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Huang Y, Tang J, Cai Z, Zhou K, Chang L, Bai Y, Ma Y. Prevotella Induces the Production of Th17 Cells in the Colon of Mice. J Immunol Res. 2020 Nov 1;2020:9607328. doi: 10.1155/2020/9607328. PMID: 33204736; PMCID: PMC7657696.
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Cui L, Guan X, Ding W, Luo Y, Wang W, Bu W, Song J, Tan X, Sun E, Ning Q, Liu G, Jia X, Feng L. Scutellaria baicalensis Georgi polysaccharide ameliorates DSS-induced ulcerative colitis by improving intestinal barrier function and modulating gut microbiota
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Wu X, Lin L, Wu H. Ferulic acid alleviates lipopolysaccharide-induced acute lung injury through inhibiting TLR4/NF-κB signaling pathway. J Biochem Mol Toxicol. 2020 Nov 2:e22664. doi: 10.1002/jbt.22664. Epub ahead of print. PMID: 33140555.
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Tian, L. X., Tang, X., Zhu, J. Y., Luo, L., Ma, X. Y., Cheng, S. W., ... & Lv, C. Z. (2020). Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis. Cell Communication and Signaling, 18, 1-16.
Cao, Y., Cao, W., Qiu, Y., Zhou, Y., Guo, Q., Gao, Y., & Lu, N. (2020). Oroxylin A suppresses ACTN1 expression to inactivate cancer-associated fibroblasts and restrain breast cancer metastasis. Pharmacological Research, 104981.
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Huang L,Shen M,Wu T,Yu Y,Yu Q,Chen Y,Xie J.Mesona chinensis Benth polysaccharides protect against oxidative stress and immunosuppression in cyclophosphamide-treated mice via MAPKs signal transduction pathways.Int J Biol Macromol.2020 Jun 1;152:766-774. do
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EK0411 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELLS, DILUTION:NA
Magnolol abrogates chronic mild stress-induced depressive-like behaviors by inhibiting neuroinflammation and oxidative stress in the prefrontal cortex of mice.
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Novel therapeutic approach by nicotine in experimental model of multiple sclerosis
Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury
Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis
Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome
From the Cover: Lung-Specific Overexpression of Constitutively Active IKK2 Induces Pulmonary and Systemic Inflammations but Not Hypothalamic Inflammation and %u2026
E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury
Whey Acidic Protein/Four-Disulfide Core Domain 21 Regulate Sepsis Pathogenesis in a Mouse Model and a Macrophage Cell Line via the Stat3/Toll-Like Receptor 4 %u2026
Exclusive enteral nutrition protects against inflammatory bowel disease by inhibiting NF%u2011%u03BAB activation through regulation of the p38/MSK1 pathway
Nobiletin attenuates lipopolysaccharide/D%u2011galactosamine%u2011induced liver injury in mice by activating the Nrf2 antioxidant pathway and subsequently inhibiting NF%u2011%u03BAB %u2026
Ascorbic acid ameliorates oxidative stress and inflammation in dextran sulfate sodium-induced ulcerative colitis in mice.
Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway?in vitro
3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation
Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial
Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice
Evaluation of the chemical composition, antioxidant and anti-inflammatory activities of distillate and residue fractions of sweet basil essential oil
Involvement of receptor-interacting protein 140 in palmitate-stimulated macrophage infiltration of pancreatic beta cells
Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1
The upregulation of integrin ?D?2 (CD11d/CD18) on inflammatory macrophages promotes macrophage retention in vascular lesions and development of atherosclerosis
High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage
A novel pH?controlled hydrogen sulfide donor protects gastric mucosa from aspirin?induced injury
H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375
Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice
5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice
Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-?B Signaling Pathway
NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-?B Signaling Pathway
In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly
MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response
LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling
Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination
Mature dendritic?cells?cause Th17/Treg imbalance by secreting TGF-?1 and IL-6 in the pathogenesis of experimental autoimmune encephalomyelitis
Surfactin induces maturation of dendritic cells in vitro
Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity
Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration
Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1? signaling pathway
MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3
4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner
Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera
Protective Effect of an Antibody against Specific Extracellular Domain of TLR2 on Agonists-Driven Inflammatory and Allergic Response
PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice
Klotho Contributes to Pravastatin Effect on Suppressing IL-6 Production in Endothelial Cells
Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway
Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-?-catenin axis
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
A deleterious role for Th9/IL-9 in hepatic fibrogenesis
Immunostimulative Activity of Low Molecular Weight Chitosans in RAW264.7 Macrophages
Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells
Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase
3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis
Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine
TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells
CD36 Participates in PrP106
Effect of Gui Zhi decoction on enteric mucosal immune in mice with collagen-induced arthritis
Inhibition of collar-induced carotid atherosclerosis by recombinant apoA-I cysteine mutants in apoE-deficient mice
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Customer Reviews
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2 Reviews For Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™
0
"Great IL-6 Kit from Boster!"
Excellent
Source: Biocompare.com
"Great IL-6 Kit from Boster!"
--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician
| Application | ELISA |
|---|---|
| Sample | Mouse serum |
| Detection | -- |
"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")
Harrison Esterly
Verified customer
Submitted 2019-12-06
0
"IL-6 Estimation in Cell Culture Supernatant"
Excellent
Source: Biocompare.com
"IL-6 Estimation in Cell Culture Supernatant"
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
| Application | ELISA |
|---|---|
| Sample | Cell culture media |
| Detection | -- |
"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")
Divya Sharma
Verified customer
Submitted 2019-05-21
Customer Q&As
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12 Customer Q&As for Mouse IL-6/Interleukin-6 ELISA Kit PicoKine™
Question
Is EK0411 applicable for human testing?
Verified customer
Asked: 2021-10-09
Answer
The Mouse IL-6 ELISA Kit PicoKine™ (EK0411) has reactivity to mouse.
Boster Scientific Support
Answered: 2021-10-10
Question
Q: which procedure should I follow in order to thaw whole blood sample for IL-6 ELISA after freezing?
G. Maharaj
Verified customer
Asked: 2020-03-14
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-6 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-03-14
Question
What standard curve (Linear, 4PL or Hyperbolic) is recommended to use in finding out the coresponding concentrations in our data using EK0411 kit?
Verified customer
Asked: 2020-01-08
Answer
For Mouse IL-6 ELISA Kit PicoKine EK0411, if your sample data points are within the middle of the curve, i.e. in the near linear region of the curve, then you can use the best fitting curve out of hyperbola, 4PL and 5PL. However, if your sample data points are near the ends of the standard curve, where it becomes not linear, it is recommended that you use 4PL for fitting, as it best fits the nature of the assay. It is also recommended that you adjust your assay so that the sample data points fall into the middle linear range of the curve. For further troubleshooting you may send us your generated data.
Boster Scientific Support
Answered: 2020-01-08
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-6 using the EK0411 Mouse IL-6 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-12-20
Question
Can you confirm if the EK0411 is compatible with ELISA microplate washers?
Verified customer
Asked: 2019-05-30
Answer
Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.
Boster Scientific Support
Answered: 2019-05-30
Question
Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?
Verified customer
Asked: 2019-05-28
Answer
For the Mouse IL-6 ELISA Kit PicoKine™ (EK0411), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.
Boster Scientific Support
Answered: 2019-05-29
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-12-11
Question
Q: Can IL-6 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-12-10
Question
Dear Sir/Madam, I want to purchase EK0411 Mouse IL-6 ELISA Kit, and my sample type is the mouse brain. Have you tested using tissues? I know as I read the description of EK0411 Mouse IL-6 ELISA Kit says it works on cell culture supernates, serum and plasma. Is it possible to test mouse brain?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
The EK0411 Mouse IL-6 ELISA Kit have been tested in liver tissue lysate but we regret to say that we do not have any related information on brain lysate. But if you want to test this EK0411 Mouse IL-6 ELISA Kit on brain tissue lysate we strongly recommend diluting the samples at least 5-fold with assay diluent to minimized any effects of the lysis buffer and the samples may need to be diluted further but you have to determined it.
Boster Scientific Support
Answered: 2017-07-24
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-12-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-12-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2015-10-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-10-08
Question
Q: if the enzyme conjugated IL-6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-6 antigen?
Verified Customer
Verified customer
Asked: 2015-02-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2015-02-09
