Mouse IL-6 PicoKine™ ELISA Kit

Reviews: 1

Product Citations: 166

Datasheet

Protocols

COA

MSDS

Place Order
SKU EK0411
Size 96wells/kit, with removable strips.
Reactivity Mouse
Sample Type cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Sensitivity <1pg/ml
Assay Range 15.6pg/ml-1000pg/ml

Overview

Product Name Mouse IL-6 PicoKine™ ELISA Kit
SKU/Catalog Number EK0411
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-6. 96wells/kit, with removable strips.
Cite This Product Mouse IL-6 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0411)
Sample Type cell culture supernates, serum and plasma( heparin, EDTA, citrate).. Anticoagulant(s): heparin, EDTA or citrate
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <1pg/ml
Assay Range 15.6pg/ml-1000pg/ml
Immunogen Expression system for standard: E.coli; Immunogen sequence: F25-T211
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from rat|polyclonal antibody from goat

Assay Details

Kit Components

Catalog numberDescriptionQuantity
EK0411-CAP96-well plate precoated with anti-Mouse IL6 antibody1
EK0411-STlyophilized recombinant Mouse IL6 standard10ng/tube
EK0411-DAbiotinylated anti-Mouse IL6 antibody130ul
AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul
AR1106-1sample diluent buffer30ml
AR1106-2antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Mouse IL-6 PicoKine™ ELISA Kit

(TMB reaction incubate at 37°C for 15-25min)

Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0320.0850.1410.2370.4520.8161.4702.281

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3615943636147482
Standard deviation2.417.7934.942.917.9340.48
CV(%)6.7%4.9%7.2%8.1%5.4%8.4%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 136393934372.125.7%
Sample 21591471541701578.385.3%
Sample 349544346443445923.465.1%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id A2RTD1
Gene Name IL6
Protein Name interleukin 6
Alternative Names Interleukin 6 ;Interleukin 6, isoform CRA_b ;Il6 ;mCG_11634 ;
Molecular Weight 24384 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

*You can search these to find other products in these research areas.
Background The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Order Product (EK0411)

Promotion:

Order 4+ of the same ELISA kit and get 20% off| Order 20+ of the same ELISA kit and get 30% off .
Option Price
1 Unit $320

USD $320

Ships in 7-10 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Customer Reviews

Review by
Rating:

Source: Biocompare.com

"IL-6 Estimation in Cell Culture Supernatant"

--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student

Application ELISA
Sample Cell culture media
Detection --

"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")

(Posted on 5/20/2019)
Write a review for EK0411

Publications

Ascorbic acid ameliorates oxidative stress and inflammation in dextran sulfate sodium-induced ulcerative colitis in mice.
PubMed ID?26884937
Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway?in vitro
PubMed ID?26617802
3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation
PubMed ID29887940
COX-2 contributes to LPS-induced Stat3 activation and IL-6 production in microglial cells
PubMed ID29636886
Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein
PubMed ID29484406
miRNA-3473b contributes to neuroinflammation following cerebral ischemia
PubMed ID29317607
VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
PubMed ID29109438
Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice
PubMed ID28977826
Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome
PubMed ID28938606
Evaluation of the chemical composition, antioxidant and anti-inflammatory activities of distillate and residue fractions of sweet basil essential oil
PubMed ID28720944
Involvement of receptor-interacting protein 140 in palmitate-stimulated macrophage infiltration of pancreatic beta cells
PubMed ID28672957
Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1
PubMed ID28586010
The upregulation of integrin ?D?2 (CD11d/CD18) on inflammatory macrophages promotes macrophage retention in vascular lesions and development of atherosclerosis
PubMed ID28500072
High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage
PubMed ID28417961
A novel pH?controlled hydrogen sulfide donor protects gastric mucosa from aspirin?induced injury
PubMed ID28387464
Exosomes derived from miR?181?5p?modified adipose?derived mesenchymal stem cells prevent liver fibrosis via autophagy activation
PubMed ID28382720
H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375
PubMed ID28382020
Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice
PubMed ID28178855
Heparanase Mediates Intestinal Inflammation and Injury in a Mouse Model of Sepsis
PubMed ID28170292
5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice
PubMed ID28138699
Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-?B Signaling Pathway
PubMed ID28119924
Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice
PubMed ID28094275
NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-?B Signaling Pathway
PubMed ID27956761
In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly
PubMed ID27847405
MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response
PubMed ID27725852
LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling
PubMed ID27590510
Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination
PubMed ID27586056
Mature dendritic?cells?cause Th17/Treg imbalance by secreting TGF-?1 and IL-6 in the pathogenesis of experimental autoimmune encephalomyelitis
PubMed ID27536199
Surfactin induces maturation of dendritic cells in vitro
PubMed ID27534429
Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity
PubMed ID27463015
Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration
PubMed ID27411313
Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1? signaling pathway
PubMed ID27398311
MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3
PubMed ID27285980
4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner
PubMed ID27276712
Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera
PubMed ID27255791
Protective Effect of an Antibody against Specific Extracellular Domain of TLR2 on Agonists-Driven Inflammatory and Allergic Response
PubMed ID27213155
PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice
PubMed ID27054331
Klotho Contributes to Pravastatin Effect on Suppressing IL-6 Production in Endothelial Cells
PubMed ID27034587
Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway
PubMed ID26990441
Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-?-catenin axis
PubMed ID26910375
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
PubMed ID26793111
A deleterious role for Th9/IL-9 in hepatic fibrogenesis
PubMed ID26728971
Immunostimulative Activity of Low Molecular Weight Chitosans in RAW264.7 Macrophages
PubMed ID26437419
Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells
PubMed ID26295043
Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase
PubMed ID26191183
A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes
PubMed ID26077651
3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis
PubMed ID25874026
CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages
PubMed ID25686540
The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice
PubMed ID25674205
Minocycline treatment ameliorates interferon-alpha- induced neurogenic defects and depression-like behaviors in mice
PubMed ID25674053
Interleukin-22 ameliorates liver fibrogenesis by attenuating hepatic stellate cell activation and downregulating the levels of inflammatory cytokines
PubMed ID25663772
Epoxyeicosatrienoic Acids Regulate Macrophage Polarization and Prevent LPS-Induced Cardiac Dysfunction
PubMed ID25626689
Sinomenine Hydrochloride Protects against Polymicrobial Sepsis via Autophagy
PubMed ID25625512
Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine
PubMed ID25450719
Terazosin activated Pgk1 and Hsp90 to promote stress resistance
PubMed ID25383758
Agmatine Protects against Zymosan-Induced Acute Lung Injury in Mice by Inhibiting NF-?B-Mediated Inflammatory Response
PubMed ID25243152
Modulating the p66shc Signaling Pathway with Protocatechuic Acid Protects the Intestine from Ischemia-Reperfusion Injury and Alleviates Secondary Liver Damage
PubMed ID24757420
Anti-Inflammatory Activity of N-Butanol Extract from?Ipomoea stolonifera In Vivo?and?In Vitro
PubMed ID24752203
Comparative Pulmonary Toxicity of Two Ceria Nanoparticles with the Same Primary Size
PubMed ID24727375
Adenosine 5?-monophosphate ameliorates?D-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice
PubMed ID24407238
Immunomodulatory Activity and Protective Effects of Polysaccharide from Eupatorium adenophorum Leaf Extract on Highly Pathogenic H5N1 Influenza Infection
PubMed ID24159339
Effect of Echium amoenum Fisch. et Mey a Traditional Iranian Herbal Remedy in an Experimental Model of Acute Pancreatitis
PubMed ID23008778
CD36 Participates in PrP106?126-Induced Activation of Microglia
PubMed ID22292032
Effect of Gui Zhi decoction on enteric mucosal immune in mice with collagen-induced arthritis
PubMed ID16149149
Inhibition of collar-induced carotid atherosclerosis by recombinant apoA-I cysteine mutants in apoE-deficient mice
PubMed ID2975715
CD36 Participates in PrP106
PubMed ID22292032
Effect of Gui Zhi decoction on enteric mucosal immune in mice with collagen-induced arthritis
PubMed ID16149149
Inhibition of collar-induced carotid atherosclerosis by recombinant apoA-I cysteine mutants in apoE-deficient mice
PubMed ID2975715
Li J, Diao B, Guo S, Huang X, Yang C, Feng Z, Yan W, Ning Q, Zheng L, Chen Y, Wu Y. Nat Commun. 2017 Nov 6;8(1):1322. doi: 10.1038/s41467-017-01327-4. VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism
PubMed ID29109438
Zhu J, Li S, Zhang Y, Ding G, Zhu C, Huang S, Zhang A, Jia Z, Li M. Am J Transl Res. 2018 Mar 15;10(3):966-974. eCollection 2018. COX-2 contributes to LPS-induced Stat3 activation and IL-6 production in microglial cells
PubMed ID29636886
Chen S, He Y, Hu Z, Lu S, Yin X, Ma X, Lv C, Jin G. J Histochem Cytochem. 2017 Apr;65(4):241-249. doi: 10.1369/0022155417692536. Epub 2017 Feb 7. Heparanase Mediates Intestinal Inflammation and Injury in a Mouse Model of Sepsis
PubMed ID28170292
Zhou W, Liu X, Zhang X, Tang J, Li Z, Wang Q, Hu R. Oncotarget. 2017 Jul 22;8(35):58903-58917. doi: 10.18632/oncotarget.19440. eCollection 2017 Aug 29. Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome
PubMed ID28938606
Lu Y, Zhang XS, Zhang ZH, Zhou XM, Gao YY, Liu GJ, Wang H, Wu LY, Li W, Hang CH. J Neuroinflammation. 2018 Mar 19;15(1):87. doi: 10.1186/s12974-018-1118-4. Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarachnoid hemorrhage
PubMed ID29554978
Zhang Y, Feng J, Cui J, Yang G, Zhu X. Mol Med Rep. 2018 Apr;17(4):5805-5813. doi: 10.3892/mmr.2018.8609. Epub 2018 Feb 15. Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein
PubMed ID29484406
Wang X, Chen S, Ni J, Cheng J, Jia J, Zhen X. Cell Death Dis. 2018 Jan 9;9(1):11. doi: 10.1038/s41419-017-0014-7. miRNA-3473b contributes to neuroinflammation following cerebral ischemia
PubMed ID29317607
Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 10.1016/j.jep.2017.12.009
PubMed ID29247698
Yan, J., Han, Z., Qu, Y., Yao, C., Shen, D., Tai, G.,..., & Zhou, Y. (2017). Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea. Food Chemistry, 240, 534-543. doi: 10.1016/j.foodchem.2017.07.154
PubMed ID-
Zhang, M., Wu, X., Xu, Y., He, M., Yang, J., Li, J.,..., & Jia, J. (2017). The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia. Brain, Behavior, and Immunity. Advance online publication. doi: 10.1016/j.bbi.2017.07.156
PubMed ID28751019
Yu, Y., Shen, M., Wang, Z., Wang, Y., Xie, M., & Xie, J. (2017). Sulfated polysaccharide from Cyclocarya paliurusenhances the immunomodulatory activity of macrophages. Carbohydrate Polymers, 174, 669-676. doi: 10.1016/j.carbpol.2017.07.009
PubMed ID-
Li Y, Fan Y, Xia B, Xiao Q, Wang Q, Sun W, Zhang H, He C. Int Immunopharmacol. 2017 Apr 19;47:206-211. doi: 10.1016/j.intimp.2017.03.031. The immunosuppressive characteristics of FB1 by inhibition of maturation and function of BMDCs.
PubMed ID28432936
Wang Z, Xie J, Yang Y, Zhang F, Wang S, Wu T, Shen M, Xie M. Sci Rep. 2017 Jan 17;7:40402. doi: 10.1038/srep40402. Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice
PubMed ID28094275
Liu T, Xiao N, Liu Y, Wilson B, Li J, Wang Y, Cao X, Liu Z. Biochem Biophys Res Commun. 2012 Oct 26;427(3):518-24. Doi: 10.1016/J.Bbrc.2012.09.088. Epub 2012 Sep 26. The Anti-Inflammatory Effects Of Ethyl Acetate On Lipopolysaccharide/D-Galactosam...
PubMed ID23022187
Xu Y, Zhou Y, Lin H, Hu H, Wang Y, Xu G. Int J Mol Med. 2013 Mar;31(3):555-60. Doi: 10.3892/Ijmm.2013.1240. Epub 2013 Jan 11. Toll-Like Receptor 2 In Promoting Angiogenesis After Acute Ischemic Injury.
PubMed ID23314218
Hasan B, Li Fs, Siyit A, Tuyghun E, Luo Jh, Upur H, Ablimit A. Respir Physiol Neurobiol. 2014 Aug 15;200:40-5. Doi: 10.1016/J.Resp.2014.05.008. Epub 2014 May 29. Expression Of Aquaporins In The Lungs Of Mice With Acute Injury Caused By Lps Treatment.
PubMed ID24879973
Li X, Liu Z, Jin H, Fan X, Yang X, Tang W, Yan J, Liang H. Biomed Res Int. 2014;2014:583736. Doi: 10.1155/2014/583736. Epub 2014 Aug 27. Agmatine Protects Against Zymosan-Induced Acute Lung Injury In Mice By Inhibiting Nf-??b-Mediated Inflammatory ...
PubMed ID25243152
Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Beari...
PubMed ID23226801
Sun H, Zhang J, Chen F, Chen X, Zhou Z, Wang H. Carbohydr Polym. 2015 May 5;121:388-402. Doi: 10.1016/J.Carbpol.2014.12.023. Epub 2015 Jan 3. Activation Of Raw264.7 Macrophages By The Polysaccharide From The Roots Of Actinidia Eriantha And Its Mol...
PubMed ID25659714
Tang Jf, Lv Xh, Wang Xl, Sun J, Zhang Yb, Yang Ys, Gong Hb, Zhu Hl. Bioorg Med Chem. 2012 Jul 15;20(14):4226-36. Doi: 10.1016/J.Bmc.2012.05.055. Epub 2012 Jun 5. Design, Synthesis, Biological Evaluation And Molecular Modeling Of Novel 1,3,4-Oxadia...
PubMed ID22727369
Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, Li C, Chen J, Li T, Wang Y. Plos One. 2014 Jun 30;9(6):E101291. Doi: 10.1371/Journal.Pone.0101291. Ecollection 2014. Ginsenoside Rg1 Prevents Cognitive Impairment And Hippocampus Senescence In A Rat Model...
PubMed ID24979747
Shen Ww, Zhao Jh. Bioelectromagnetics. 2010 Feb;31(2):113-9. Doi: 10.1002/Bem.20535. Pulsed Electromagnetic Fields Stimulation Affects Bmd And Local Factor Production Of Rats With Disuse Osteoporosis.
PubMed ID19670410
Zhou Qb, Jin Yl, Jia Q, Zhang Y, Li Ly, Liu P, Liu Yt. Inflammation. 2014 Feb;37(1):107-15. Doi: 10.1007/S10753-013-9717-9. Baicalin Attenuates Brain Edema In A Rat Model Of Intracerebral Hemorrhage.
PubMed ID23974988
Asgary S, Keshvari M, Afshani Mr, Amiri M, Laher I, Javanmard Sh. Isrn Nutr. 2014 Mar 4;2014:405867. Doi: 10.1155/2014/405867. Ecollection 2014. Effect Of Fresh Orange Juice Intake On Physiological Characteristics In Healthy Volunteers.
PubMed ID24967267
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
PubMed ID25674205
Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.
PubMed ID24137221
Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...
PubMed ID22509346
Meng H, Shen Y, Shen J, Zhou F, Shen S, Das Un. Lipids Health Dis. 2013 Oct 29;12:160. Doi: 10.1186/1476-511X-12-160. Effect Of N-3 And N-6 Unsaturated Fatty Acids On Prostate Cancer (Pc-3) And Prostate Epithelial (Rwpe-1) Cells In Vitro.
PubMed ID24168453
Wang M, Zheng Z, Meng J, Wang H, He M, Zhang F, Liu Y, Hu B, He Z, Hu Q, Wang H. Biomaterials. 2015 Sep;64:78-87. Doi: 10.1016/J.Biomaterials.2015.06.030. Epub 2015 Jun 20. In Vivo Study Of Immunogenicity And Kinetic Characteristics Of A Quantum D...
PubMed ID26117660
Chen J, Xu J, Li J, Du L, Chen T, Liu P, Peng S, Wang M, Song H. Int Immunopharmacol. 2015 May;26(1):147-52. Doi: 10.1016/J.Intimp.2015.03.025. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Attenuates Lipopolysaccharide-Induced Mastitis In Rats Via...
PubMed ID25840281
Wang Y, Zhang Zz, Wu Y, Zhan J, He Xh, Wang Yl. Exp Ther Med. 2013 Jan;5(1):315-319. Epub 2012 Oct 25. Honokiol Protects Rat Hearts Against Myocardial Ischemia Reperfusion Injury By Reducing Oxidative Stress And Inflammation.
PubMed ID23251290
Zhan Y, Wang Z, Yang P, Wang T, Xia L, Zhou M, Wang Y, Wang S, Hua Z, Zhang J. Cell Death Dis. 2014 Jan 9;5:E985. Doi: 10.1038/Cddis.2013.516. Adenosine 5'-Monophosphate Ameliorates D-Galactosamine/Lipopolysaccharide-Induced Liver Injury Through A...
PubMed ID24407238
Ma L, Wang G, Chen Z, Li Z, Yao J, Zhao H, Wang S, Ma Z, Chang H, Tian X. Scientificworldjournal. 2014 Mar 16;2014:387640. Doi: 10.1155/2014/387640. Ecollection 2014. Modulating The P66Shc Signaling Pathway With Protocatechuic Acid Protects The In...
PubMed ID24757420
Ying Hz, Liu Yh, Yu B, Wang Zy, Zang Jn, Yu Ch. Food Chem Toxicol. 2013 Feb;52:53-60. Doi: 10.1016/J.Fct.2012.10.030. Epub 2012 Nov 2. Dietary Quercetin Ameliorates Nonalcoholic Steatohepatitis Induced By A High-Fat Diet In Gerbils.
PubMed ID23123425
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Transl Res. 2010 Dec;156(6):326-34. Doi: 10.1016/J.Trsl.2010.09.001. Epub 2010 Oct 1. Nicotine Suppresses Inflammatory Factors In Hbe16 Airway Epithelial Cells After Exposure To Cigarette Smoke Extract And Li...
PubMed ID21078494
Dong Gh, Gong Jp, Li Jz, Luo Yh, Li Zd, Li Pz, He K. Inflammation. 2013 Oct;36(5):1087-93. Doi: 10.1007/S10753-013-9641-Z. Association Between Gene Polymorphisms Of Irak-M And The Susceptibility Of Sepsis.
PubMed ID23588345
Li X, Peng F, Xie C, Wu W, Han X, Chen L. Int Immunopharmacol. 2013 Dec;17(4):1125-33. Doi: 10.1016/J.Intimp.2013.10.001. Epub 2013 Oct 14. (E)-3-(3,4-Dimethoxyphenyl)-1-(5-Hydroxy-2,2-Dimethyl-2H-Chromen-6-Yl)Prop-2-En-1-One Ameliorates The Colla...
PubMed ID24135236
Guo R, Wu K, Chen J, Mo L, Hua X, Zheng D, Chen P, Chen G, Xu W, Feng J. Cell Physiol Biochem. 2013;32(6):1668-80. Doi: 10.1159/000356602. Epub 2013 Dec 13. Exogenous Hydrogen Sulfide Protects Against Doxorubicin-Induced Inflammation And Cytotoxic...
PubMed ID24356372
Wang G, Chen Z, Zhang F, Jing H, Xu W, Ning S, Li Z, Liu K, Yao J, Tian X. Apoptosis. 2014 Sep;19(9):1342-53. Doi: 10.1007/S10495-014-1008-X. Blockade Of Pkc?? Protects Against Remote Organ Injury Induced By Intestinal Ischemia And Reperfusion Via ...
PubMed ID24930012
Wei Z, Yang Y, Xie C, Li C, Wang G, Ma L, Xiang M, Sun J, Wei Y, Chen L. Fitoterapia. 2014 Sep;97:172-83. Doi: 10.1016/J.Fitote.2014.06.002. Epub 2014 Jun 9. Synthesis And Biological Evaluation Of Pyranoisoflavone Derivatives As Anti-Inflammatory ...
PubMed ID24924289
Xu W, Lu Y, Yao J, Li Z, Chen Z, Wang G, Jing H, Zhang X, Li M, Peng J, Tian X. Shock. 2014 Nov;42(5):440-7. Doi: 10.1097/Shk.0000000000000225. Novel Role Of Resveratrol: Suppression Of High-Mobility Group Protein Box 1 Nucleocytoplasmic Transloca...
PubMed ID25004063
Sun Ln, Yang Zy, Lv Ss, Liu Xc, Guan Gj, Liu G. Int Immunopharmacol. 2014 Nov;23(1):236-46. Doi: 10.1016/J.Intimp.2014.08.023. Epub 2014 Sep 4. Curcumin Prevents Diabetic Nephropathy Against Inflammatory Response Via Reversing Caveolin-1 Tyr14 Pho...
PubMed ID25196431
Zhang F, Li Zl, Xu Xm, Hu Y, Yao Jh, Xu W, Jing Hr, Wang S, Ning Sl, Tian Xf. Mol Med Rep. 2015 Jan;11(1):269-76. Doi: 10.3892/Mmr.2014.2679. Epub 2014 Oct 16. Protective Effects Of Icariin-Mediated Sirt1/Foxo3 Signaling Pathway On Intestinal Isch...
PubMed ID25323330
Ni J, Wang X, Chen S, Liu H, Wang Y, Xu X, Cheng J, Jia J, Zhen X. Brain Behav Immun. 2015 Oct;49:75-85. Doi: 10.1016/J.Bbi.2015.04.014. Epub 2015 Apr 29. Microrna Let-7C-5P Protects Against Cerebral Ischemia Injury Via Mechanisms Involving The In...
PubMed ID25934573
Jin Y, Zhang Y, Wan C, Wang H, Hou L, Chang J, Fan K, Xie X. Evid Based Complement Alternat Med. 2013;2013:194976. Doi: 10.1155/2013/194976. Epub 2013 Sep 18. Immunomodulatory Activity And Protective Effects Of Polysaccharide From Eupatorium Adeno...
PubMed ID24159339
Lu Dh, Guo Xy, Qin Sy, Luo W, Huang Xl, Chen M, Wang Jx, Ma Sj, Yang Xw, Jiang Hx. World J Gastroenterol. 2015 Feb 7;21(5):1531-45. Doi: 10.3748/Wjg.V21.I5.1531. Interleukin-22 Ameliorates Liver Fibrogenesis By Attenuating Hepatic Stellate Cell Ac...
PubMed ID25663772
Xie Wl, Shi Q, Zhang J, Zhang By, Gong Hs, Guo Y, Wang Sb, Xu Y, Wang K, Chen C, Liu Y, Dong Xp. J Mol Neurosci. 2013 Nov;51(3):919-32. Doi: 10.1007/S12031-013-0002-Z. Epub 2013 Mar 24. Abnormal Activation Of Microglia Accompanied With Disrupted C...
PubMed ID23526370
Cai C, Chen Y, Zhong S, Ji B, Wang J, Bai X, Shi G. Plos One. 2014 Apr 21;9(4):E95931. Doi: 10.1371/Journal.Pone.0095931. Ecollection 2014. Anti-Inflammatory Activity Of N-Butanol Extract From Ipomoea Stolonifera In Vivo And In Vitro.
PubMed ID24752203
Fan Z, Jing H, Yao J, Li Y, Hu X, Shao H, Shen G, Pan J, Luo F, Tian X. Oxid Med Cell Longev. 2014;2014:191624. Doi: 10.1155/2014/191624. Epub 2014 Aug 20. The Protective Effects Of Curcumin On Experimental Acute Liver Lesion Induced By Intestinal...
PubMed ID25215173
Zheng Ls, Kaneko N, Sawamoto K. Front Cell Neurosci. 2015 Jan 28;9:5. Doi: 10.3389/Fncel.2015.00005. Ecollection 2015. Minocycline Treatment Ameliorates Interferon-Alpha- Induced Neurogenic Defects And Depression-Like Behaviors In Mice.
PubMed ID25674053
Kang X, Li Y, Wei J, Zhang Y, Bian C, Wang K, Wu X, Hu Y, Li J, Yang Y. Plos One. 2013 Nov 1;8(11):E77427. Doi: 10.1371/Journal.Pone.0077427. Ecollection 2013. Elevation Of Matrix Metalloproteinase-9 Level In Cerebrospinal Fluid Of Tick-Borne Ence...
PubMed ID24223711
Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regulati...
PubMed ID26113783
Xu R, Tao A, Zhang S, Zhang M. Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):726-33. Doi: 10.1093/Abbs/Gmt065. Epub 2013 Jun 19. Neutralization Of Interleukin-17 Attenuates High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease In Mice.
PubMed ID23786840
Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...
PubMed ID23022502
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.
PubMed ID24955162
Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...
PubMed ID23746956
Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.
PubMed ID24710706
Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...
PubMed ID25725585
Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.
PubMed ID25383758
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
PubMed ID26022197
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.
PubMed ID25626689
Zhang Y, Zhang S, Li H, Huang M, Xu W, Chu K, Chen L, Chen X. Mol Med Rep. 2015 Jul;12(1):988-94. Doi: 10.3892/Mmr.2015.3515. Epub 2015 Mar 19. Ameliorative Effects Of Gualou Guizhi Decoction On Inflammation In Focal Cerebral Ischemic-Reperfusion ...
PubMed ID25815894
Shi Jq, Chen J, Wang Br, Zhu Yw, Xu Y, Wang J, Xiao H, Shi Jp, Zhang Yd, Xu J. Peptides. 2011 Aug;32(8):1617-25. Doi: 10.1016/J.Peptides.2011.06.013. Epub 2011 Jun 21. Short Amyloid-Beta Immunogens Show Strong Immunogenicity And Avoid Stimulating ...
PubMed ID21722679
Yang C, Ling H, Zhang M, Yang Z, Wang X, Zeng F, Wang C, Feng J. Mol Cells. 2011 Jun;31(6):531-8. Doi: 10.1007/S10059-011-1025-3. Epub 2011 Apr 20. Oxidative Stress Mediates Chemical Hypoxia-Induced Injury And Inflammation By Activating Nf-??b-Cox-...
PubMed ID21533553
Hu Y, Chen X, Duan H, Hu Y, Mu X. Immunopharmacol Immunotoxicol. 2009;31(4):550-5. Doi: 10.3109/08923970902814129. Chinese Herbal Medicinal Ingredients Inhibit Secretion Of Il-6, Il-8, E-Selectin And Txb2 In Lps-Induced Rat Intestinal Microvascula...
PubMed ID19874221
Yang J, Yu Hm, Zhou Xd, Kolosov Vp, Perelman Jm. Mol Immunol. 2013 Jan;53(1-2):161-71. Doi: 10.1016/J.Molimm.2012.06.015. Epub 2012 Aug 30. Study On Trpv1-Mediated Mechanism For The Hypersecretion Of Mucus In Respiratory Inflammation.
PubMed ID22935106
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Cell Biochem Biophys. 2012 Apr;62(3):421-31. Doi: 10.1007/S12013-011-9324-Z. The Expression And Pharmacological Characterization Of Nicotinic Acetylcholine Receptor Subunits In Hbe16 Airway Epithelial Cells.
PubMed ID22095388
Yu J, Lu Y, Li Y, Xiao L, Xing Y, Li Y, Wu L. J Pharm Pharmacol. 2015 Sep;67(9):1240-50. Doi: 10.1111/Jphp.12415. Epub 2015 Apr 16. Role Of S100A1 In Hypoxia-Induced Inflammatory Response In Cardiomyocytes Via Tlr4/Ros/Nf-??b Pathway.
PubMed ID25880347
Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P...
PubMed ID23171368
Zhang H, Xia X, Han F, Jiang Q, Rong Y, Song D, Wang Y. Mol Pharm. 2015 May 4;12(5):1648-61. Doi: 10.1021/Acs.Molpharmaceut.5B00069. Epub 2015 Apr 2. Cathelicidin-Bf, A Novel Antimicrobial Peptide From Bungarus Fasciatus, Attenuates Disease In A D...
PubMed ID25807257
Abed A, Minaiyan M, Ghannadi A, Mahzouni P, Babavalian Mr. Isrn Gastroenterol. 2012;2012:141548. Epub 2012 Sep 13. Effect Of Echium Amoenum Fisch. Et Mey A Traditional Iranian Herbal Remedy In An Experimental Model Of Acute Pancreatitis.
PubMed ID23008778
Li P, Wang B, Sun F, Li Y, Li Q, Lang H, Zhao Z, Gao P, Zhao Y, Shang Q, Liu D, Zhu Z. Sci Rep. 2015 May 28;5:10229. Doi: 10.1038/Srep10229. Mitochondrial Respiratory Dysfunctions Of Blood Mononuclear Cells Link With Cardiac Disturbance In Patient...
PubMed ID26018291
Zhuang Xf, Yin Cq, Wang Hy, Sun Nl. J Int Med Res. 2009 Sep-Oct;37(5):1354-64. Distinctive Effects Of Carvedilol In The Non-Infarct Zone: Remodelling Of The Ligated Rat Heart Linked To Oxidative Stress.
PubMed ID19930840
Zhang X, Zhu X, Chen B. J Lipid Res. 2010 Dec;51(12):3434-42. Doi: 10.1194/Jlr.M008573. Epub 2010 Sep 3. Inhibition Of Collar-Induced Carotid Atherosclerosis By Recombinant Apoa-I Cysteine Mutants In Apoe-Deficient Mice.
PubMed ID20817832
Shirpoor A, Norouzi L, Khadem Ansari Mh, Ilkhanizadeh B, Gharaaghaji R. Int Immunopharmacol. 2013 Aug;16(4):498-504. Doi: 10.1016/J.Intimp.2013.04.024. Epub 2013 May 9. Vasoprotective Effect Of Vitamin E: Rescue Of Ethanol-Induced Atherosclerosis ...
PubMed ID23665315
Wang Y, Guo W, Li Y, Pan X, Lv W, Cui L, Li C, Wang Y, Yan S, Zhang J, Liu B. J Gastroenterol Hepatol. 2014 Apr;29(4):742-8. Doi: 10.1111/Jgh.12448. Hypothermia Induced By Adenosine 5'-Monophosphate Attenuates Injury In An L-Arginine-Induced Acute...
PubMed ID24224980
Wang Y, Song G, Wang Y, Qiu L, Qin X, Liu H, Li F, Wang X, Li F, Guo S, Zhang Y, Li Z. J Proteome Res. 2014 Feb 7;13(2):710-9. Doi: 10.1021/Pr4008255. Epub 2013 Dec 17. Elevated Serum Levels Of Circulating Immunoinflammation-Related Protein Comple...
PubMed ID24295561
Peng L, He X, Zhang P, Zhang J, Li Y, Zhang J, Ma Y, Ding Y, Wu Z, Chai Z, Zhang Z. Int J Mol Sci. 2014 Apr 10;15(4):6072-85. Doi: 10.3390/Ijms15046072. Comparative Pulmonary Toxicity Of Two Ceria Nanoparticles With The Same Primary Size.
PubMed ID24727375
Wang G, Ye Y, Zhang X, Song J. Oncol Rep. 2014 Oct;32(4):1709-14. Doi: 10.3892/Or.2014.3366. Epub 2014 Jul 30. Bradykinin Stimulates Il-6 Production And Cell Invasion In Colorectal Cancer Cells.
PubMed ID25109623
Wang Yh, Xuan Zh, Tian S, Du Gh. Evid Based Complement Alternat Med. 2015;2015:189239. Doi: 10.1155/2015/189239. Epub 2015 Feb 18. Echinacoside Protects Against 6-Hydroxydopamine-Induced Mitochondrial Dysfunction And Inflammatory Responses In Pc12...
PubMed ID25788961
Fan Z, Yao J, Li Y, Hu X, Shao H, Tian X. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3451-9. Ecollection 2015. Anti-Inflammatory And Antioxidant Effects Of Curcumin On Acute Lung Injury In A Rodent Model Of Intestinal Ischemia Reperfusion By Inhibitin...
PubMed ID26097529
Xu Ll,, Zhu Jf,, Xu Xl,, Zhu J,, Li L,, Xi My,, Jiang Zy,, Zhang My,, Liu F,, Lu Mc,, Bao Qc,, Li Q, Zhang C,, Wei Jl,, Zhang Xj,,, Zhang Ls, You Qd,,, Sun Hp,,. J Med Chem. 2015 Jul 23;58(14):5419-36. Doi: 10.1021/Acs.Jmedchem.5B00170. Epub 2015 ...
PubMed ID26111355
Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And...
PubMed ID25953665
Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...
PubMed ID24368037
Wang Xj, Deng Hz, Jiang B, Yao H. Int J Colorectal Dis. 2012 May;27(5):575-81. Doi: 10.1007/S00384-011-1352-Z. Epub 2011 Nov 15. The Natural Plant Product Sophocarpine Ameliorates Dextran Sodium Sulfate-Induced Colitis In Mice By Regulating Cytoki...
PubMed ID22083209
Huang J, Wang P, Li M, Ge J, Chen J, Chen X. Int J Mol Med. 2015 Aug;36(2):493-500. Doi: 10.3892/Ijmm.2015.2249. Epub 2015 Jun 16. Trichostatin A Reduces Cisplatin-Induced Ototoxicity Through The Stat6 Signaling Pathway.
PubMed ID26080623
Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.
PubMed ID24571896
Hu W,, Jing P, Wang L, Zhang Y, Yong J, Wang Y. Bmc Complement Altern Med. 2015 Apr 15;15:119. Doi: 10.1186/S12906-015-0642-3. The Positive Effects Of Ginsenoside Rg1 Upon The Hematopoietic Microenvironment In A D-Galactose-Induced Aged Rat Model.
PubMed ID25881060
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????
PubMed ID25428340
Li J, Huang M, Shen X. J Diabetes Complications. 2014 Sep-Oct;28(5):662-6. Doi: 10.1016/J.Jdiacomp.2014.06.008. Epub 2014 Jun 19. The Association Of Oxidative Stress And Pro-Inflammatory Cytokines In Diabetic Patients With Hyperglycemic Crisis.
PubMed ID25044235
Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo...
PubMed ID23642811
Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli...
PubMed ID24685824
Duan F, Liao J, Huang Q, Nie Y, Wu K. Clin Dev Immunol. 2012;2012:192791. Doi: 10.1155/2012/192791. Epub 2012 Apr 9. Hsv-1 Mir-H6 Inhibits Hsv-1 Replication And Il-6 Expression In Human Corneal Epithelial Cells In Vitro.
PubMed ID22550533
Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.
PubMed ID24656779
Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...
PubMed ID23219582
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induced...
PubMed ID21647942
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
PubMed ID26077651
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.
PubMed ID23382627

Customer Q&As

Q: The volumes of my samples are small, would it be possible to use 50µl/sample?
A: There are two solutions for insufficient sample volume.
1. Diluent the samples with the provided sample diluent buffer into 100ul.
2. Add 50ul of standard solution, when 50ul of sample will be added into a well.
Q: Will this kit work on tissue samples?
A: For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit.
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
Q: Is it possible to dilute 10X TBS stock that is 250mM Tris Base/Tris-Hcl, 27 mM KCl, and 1.37M NaCl to be used as the wash buffer?
A: You can dilute the 10X TBS stock to 1XTBS and test the pH value of the 1xTBS.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
Q: Would your kits work normally with samples from Wistar Rat?
A: Our kits would work normally with samples from Wistar Rat.
Q: How can I store the dissolved wash buffer for a longer period of time?
A: You can prepare a concentrated wash buffer (25X) which can be stored at 4°C for 1-3 months.
Q: Does the TMB color developing agent contain H2O2?
A: Yes, the TMB color developing agent contain H2O2.
Q: Does this ELISA kit contain any product produced in humans or primates?
Keywords: component, ingredient
A: None of the components in our ELISA kits are produced in humans or primates.
Q: What is the volume of the recombinant protein control? What is its shelf life?
Keywords: expiration, storage, temperature, size
A: The volume of the control is around 100pg. If unopened, the shelf life of the control is the same as the whole kit - "Store at 4˚C for 6 months, at -20˚C for 12 months." The reconstituted control can be stored at -20˚C for 2 days.
Q: Will it be okay to run the experiment if I accidentally used the wrong buffer (e.g. sample diluent buffer) for antibody dilution instead of the antibody diluent buffer? Keywords: dilution, replace, substitute, sample buffer
A: Using the sample diluent buffer for antibody dilution instead of the antibody diluent buffer will negatively affect the test result to some extent. The values of both standard and sample might be lower than the normal values.
Q: The protocol says to use neutral PBS and provides a recipe. Could I use neutral PBS made with KCl or KH2PO4 (common constituents for most PBS recipes) instead? Keywords: protocol, alternative buffer, applications, reagent recipe
A: Yes, it is ok to use common PBS recipes. We have tried many types of buffers with common constituents, and no significant difference was observed whatsoever.
Q: My plasma samples have been somewhat hemolyzed. Does plasma hemolysis have any effect on ELISA results? Keyword: application, protocol, troubleshooting
A: There will be some unspecific staining in hemolysis samples and make the result inaccurate. We suggest you collect plasma samples again, hemolysis samples are not recommended to use.
Q: Why are my O.D. values different than your values on the datasheet? Keyword: troubleshooting, reference
A: We detected the kit in our lab and got our values on the datasheet before delivery, but protein activity will decrease as time goes on, so you may get lower O.D. values than ours. However, it should still be in a reasonable range and the standards can be used to calculate sample values. Good linearity of curve is more important than the actual numerical value.
Q: On the ELISA kit datasheet it says "HRP substrate TMB was used to visualize HRP enzymatic reaction." What is HRP and which part of the kit contains HRP? Keyword: kit components, reagents, protocol
A: It means Avidin-Biotin-Peroxidase Complex(AR1103) and you could find it in Kit Components.
Q: Do you offer any ELISA kits that can work with whole blood samples instead of plasma or urine? Keyword: applications
A: We test serum and plasma routinely, and there is very little difference between serum, plasma and whole blood. The whole blood also contains proteins we need to test. The kit can be used to test whole blood in theory.
Q: Does silicic acid (formula SiOH4) affect the results of the ELISA assay? Keyword: protocol, reagents
A: The acid may affect the binding of antigen to antibody, it is not recommended to use. That being said, it is unlikely to affect the reaction if the solution remains an overall neutral environment.
Q: Is there lot-to-lot variation of the ELISA kits? What are Boster's general services when I have questions about your kits? Keyword: technical support, help, customer service
A: The NIBSC/WHO 1st International Standard is evaluated, we always test our kits before delivery, and customers can find the test result on the protocol. If the customer needs technical support from us to analyze their data, please contact us and we ask that you provide us the following information: the lot# and production date, and when did the customer detect the kit.
Q: The results of my standards do not look correct, what could be the problem? Keyword: troubleshoot, protocol
A: Double check the protocol for plate washing. It should be examined on three aspects: 1) Did you make the washing buffer correctly? 2) How long did you let the buffer stay in wells? 3) What was the volume of the washing buffer added to each well? In addition, pay attention when adding sample to avoid contamination. And we suggest testing the standards again. Values of standards at low concentration are more affected than that at higher concentration, so customer can still get expected values at high concentration even if there is an error. If problems persist, please contact technical support with the catalog and lot number of your product.
Q: What is the well depth of the 96 well plates for the ELISA kits? Keyword: well capacity, product size
A: The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.
Q: The kit does not include wash solution, what should I do? Keyword: wash buffer
A: Our Elisa kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder SKU: AR0030).
Q: For your ELISA kits, are the capture and detection antibodies polyclonals or monoclonals? Keyword: antibody clonality
A: This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.
Q: Do your ELISA kits come with sealants or plate covers? Keyword: storage, sequential use
A: The kit can be used within a month sequentially if it's opened and stored at 4 degree. There is no need to use sealants, the plate can be packaged with aluminum foil bag, and for other reagents keep bottle tightly closed.
Q: What is the concentration of Sodium Azide in your Elisa kits? Keyword: preservative
A: Our Elisa kits contain 0.02% Sodium Azide. All of the components except TMB colour developing reagent and stop solution contains 0.02% Sodium azide as the preservative.
Q: Are there positive and negative controls available for my ELISA kit? Keyword: positive control, negative control
A: We can provide a recombinant protein as a control. It costs $50 per control and takes 2 weeks to manufacture. We cannot provide a positive or negative control in serum.
Q: Why do I get positive results for my knockout (KO) model when used as a control?
A: The knockout (KO) model may contain truncated forms of the target protein which can be detected by the ELISA assay.
Q: How long do I soak my plate in the wash buffer?
A: The plate should soak in 0.3mL PBS or TBS wash buffer for at least 1-2 minutes in an automated wash station.
Q: Can I use Tween in my wash buffer?
A: While it is not recommended to use Tween in your wash buffer, small amounts (<0.1% concentration) may decrease background due to insufficient blocking.
Q: What plate type do I use to set up the microplate reader?
A: Our plates are made with the Corning costar plates similar to the DNA-BIND 96 -well plates.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the normal level of this protein in my sample of interest?
A: We have reviewed literature and have gathered this information for most of our ELISA kits. You can find this information on the product page or contact us if you cannot find it. However this information is only suggestive and cannot replace pilot studies in determining the optimal sample dilution ratio.
Q: Is the plate separable? Can I use only part of the kit and save the rest for later? How many samples can I run with one kit?
A: Yes the plate is separable. There are 12 strips of 8 wells each, all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit. Contact us if you have questions regarding other situations.
Q: Does the kit contain sample preparation reagents? How do I prepare my samples?
A: Since different sample types require different reagents, we do not include them in the ELISA kit. However we do have each reagent mentioned in the file below available at very reasonable prices. Be sure to check them out. For sample preparation protocols please download the file below: https://www.bosterbio.com/media/pdf/Boster_Sample_Preparation_Protocols.pdf
Q: Can this ELISA kit work on brain tissue homogenate, cell culture supernatant, saliva, urine, serum, whole blood or any other sample type?
A: In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. If you want dilution ratio suggestions on these sample types please contact our technical support. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Q: Can this ELISA kit react with the pro-form of the target protein? Can this ELISA kit react with an isoform of the protein?
A: In general, unless otherwise specified, the ELISA kit is pan-specific, meaning that it will react with all different forms of the target protein if they share the majority of the target protein's sequence. The capture and detection antibodies are reactive to the entire sequence of the standard protein. You can find the sequence information of the standard protein in the "immunogen" section. For more information about the specificity of the kit for your particular experiment, please contact our technical support.
Q: Can this ELISA kit react with human, mouse, rat or other species?
A: If the kit is reactive to another commonly used species (human, mouse, and/or rat), we would have listed it as a separate product. If you want to check cross-reactivity to a species that is not included in the 3 species listed above, please contact our technical support for more information. As a rule of thumb, if the sequences are 90%+ identical, there is a high chance of cross-reactivity for your species of interest.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mouse il 6 elisa kit, mouse il-6 elisa kit, mouse il6 elisa kit, mouse interleukin 6 elisa kit, mouse interleukin-6 elisa kit